α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata)

Wang, Shanshan; Saito, Takanori; Ohkawa, Katsuya; Ohara, Hitoshi; Shishido, Masahiro; Ikeura, Hiromi; Takagi, Kazuteru; Ogawa, Satoru; Yokoyama, Mineyuki; Kondo, Satoru

doi:10.1016/j.jplph.2016.01.009

Cited by 13 publications

(3 citation statements)

References 46 publications

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“…While this study provided strong evidence for the potential relevance of 9,10-KODA to SAR induction, it is unknown if 9,10-KODA acts independently of SA on the induction of SAR marker genes. A further report supported the defensive role of 9,10-KODA as an exogenous application of 9,10-KODA to grape berries suppressed the disease progression by Glomerella cingulata Stonem [41]. Recent genetic and pharmacological evidence obtained by the analysis of maize knockout mutants identified 9,10-KODA as a major xylem-mobile long-distance signal required for the activation of induced systemic resistance (ISR) against leaf pathogens triggered by root colonization with the beneficial fungal symbiont Trichoderma virens [42], suggesting a signaling role for this molecule.…”

Section: Ketols Serve Signaling Roles In Plant-pathogen Interactionsmentioning

confidence: 60%

Ketols Emerge as Potent Oxylipin Signals Regulating Diverse Physiological Processes in Plants

Berg-Falloure

Kolomiets

2023

Plants

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Plants produce an array of oxylipins implicated in defense responses against various stresses, with about 600 oxylipins identified in plants to date. Most known oxylipins are the products of lipoxygenase (LOX)-mediated oxygenation of polyunsaturated fatty acids. One of the most well-characterized oxylipins produced by plants is the hormone jasmonic acid (JA); however, the function of the vast majority of oxylipins remains a mystery. One of the lesser-studied groups of oxylipins is comprised of ketols produced by the sequential action of LOX, allene oxide synthase (AOS), followed by non-enzymatic hydrolysis. For decades, ketols were mostly considered mere by-products of JA biosynthesis. Recent accumulating evidence suggests that ketols exhibit hormone-like signaling activities in the regulation of diverse physiological processes, including flowering, germination, plant–symbiont interactions, and defense against biotic and abiotic stresses. To complement multiple reviews on jasmonate and overall oxylipin biology, this review focuses specifically on advancing our understanding of ketol biosynthesis, occurrence, and proposed functions in diverse physiological processes.

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Section: Ketols Serve Signaling Roles In Plant-pathogen Interactionsmentioning

confidence: 60%

Ketols Emerge as Potent Oxylipin Signals Regulating Diverse Physiological Processes in Plants

Berg-Falloure

Kolomiets

2023

Plants

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“…The formation of the biologically active jasmonoyl-isoleucine (JA-Ile) is catalyzed by Jasmonate Resistant 1 (JAR1) in the cytoplasm [ 29 ]. In addition to JA, AOS produces ketols, a group of C18-oxylipin compounds with hormone-like signaling activities that were recently shown to play essential roles in defense against fungal pathogens and chewing insects [ 30 , 31 , 32 , 33 , 34 , 35 ]. Some ketols, including 9-hydroxy-10-oxo-12( Z ), 15( Z )-octadecadienoic acid (9,10-KODA), 9-hydroxy-12-oxo-10( E ), 15( Z )-octadecadienoic acid (9-12-KODA), and 9-hydroxy-12-oxo-10( E )-octadecenoic acid (9-12-KOMA), have been shown to prime plants for defense against pathogens [ 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Maize OPR2 and LOX10 Mediate Defense against Fall Armyworm and Western Corn Rootworm by Tissue-Specific Regulation of Jasmonic Acid and Ketol Metabolism

Huang,

Grunseich,

Berg-Falloure

et al. 2023

Genes

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Foliage-feeding fall armyworm (FAW; Spodoptera frugiperda) and root-feeding western corn rootworm (WCR; Diabrotica virgifera virgifera) are maize (Zea mays L.) pests that cause significant yield losses. Jasmonic acid (JA) plays a pivotal defense role against insects. 12-oxo-phytodienoic acid (12-OPDA) is converted into JA by peroxisome-localized OPDA reductases (OPR). However, little is known about the physiological functions of cytoplasmic OPRs. Here, we show that disruption of ZmOPR2 reduced wound-induced JA production and defense against FAW while accumulating more JA catabolites. Overexpression of ZmOPR2 in Arabidopsis enhanced JA production and defense against beet armyworm (BAW; Spodoptera exigua). In addition, lox10opr2 double mutants were more susceptible than either single mutant, suggesting that ZmOPR2 and ZmLOX10 uniquely and additively contributed to defense. In contrast to the defensive roles of ZmOPR2 and ZmLOX10 in leaves, single mutants did not display any alteration in root herbivory defense against WCR. Feeding on lox10opr2 double mutants resulted in increased WCR mortality associated with greater herbivory-induced production of insecticidal death acids and ketols. Thus, ZmOPR2 and ZmLOX10 cooperatively inhibit the synthesis of these metabolites during herbivory by WCR. We conclude that ZmOPR2 and ZmLOX10 regulate JA-mediated resistance in leaves against FAW while suppressing insecticidal oxylipin synthesis in roots during WCR infestation.

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“…The application of KODA was initially shown to promote flowering in various plants such as Lemna paucicostata ( Yokoyama et al , 2000 ), Malus pamila ( Kittikorn et al , 2011 , 2013 ), Citrus unshiu ( Nakajima et al , 2016 ), and Pharbitis nil ( Ono et al , 2013 ). KODA also promotes rooting ( Kawakami et al , 2015 ), re-shooting after dormancy ( Sakamoto et al , 2010 , 2012 ; Nakajima et al , 2016 ), and it also increases wheat yield ( Haque et al , 2016 ) and enhances the resistance of certain herbaceous plants to pathogens ( Endo et al , 2013 ; Wang et al , 2016 , 2020 ). Although these findings suggest the agricultural usefulness of KODA, technical innovation is still needed to decrease the manufacturing cost, thereby ensuring a sufficient supply for practical uses of KODA in agriculture.…”

Section: Introductionmentioning

confidence: 99%

Developing a platform for production of the oxylipin KODA in plants

Ihara

Wakamatsu

Yokoyama

et al. 2021

Journal of Experimental Botany

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KODA (9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid) is a plant oxylipin involved in recovery from stress. As an agrichemical, KODA helps maintain crop production under various environmental stresses. In plants, KODA is synthesized from α-linolenic acids via 9-lipoxygenase (9-LOX) and allene oxide synthase (AOS), although the amount is usually low except in the free-floating aquatic plant Lemna paucicostata. To improve KODA biosynthetic yield in other plants such as Nicotiana benthamiana and Arabidopsis thaliana, we developed a system to overproduce KODA in vivo via ectopic expression of L. paucicostata 9-LOX and AOS. The transient expression in N. benthamiana showed that the expression of these two genes is sufficient to produce KODA in leaves. However, stable expression of 9-LOX and AOS (with consequent KODA production) in Arabidopsis plants succeeded only when the two proteins were localized in plastids or the endoplasmic reticulum/lipid droplets. Although only small amounts of KODA could be detected in leaf extracts of transgenic Nicotiana or Arabidopsis plants, subsequent incubation of the extracts increased KODA abundance over time. Therefore, KODA production in transgenic plants stably expressing 9-LOX and AOS requires specific subcellular localization of these two enzymes and incubation of leaf crude extracts, which liberates α-linolenic acid via breakdown of endogenous lipids.

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α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata)

Cited by 13 publications

References 46 publications

Ketols Emerge as Potent Oxylipin Signals Regulating Diverse Physiological Processes in Plants

Ketols Emerge as Potent Oxylipin Signals Regulating Diverse Physiological Processes in Plants

Maize OPR2 and LOX10 Mediate Defense against Fall Armyworm and Western Corn Rootworm by Tissue-Specific Regulation of Jasmonic Acid and Ketol Metabolism

Developing a platform for production of the oxylipin KODA in plants

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