α-Human atrial natriuretic polypeptide is released from the heart and circulates in the body

Sugawara, Akira; Nakao, Kazuwa; Morii, Narito; Sakamoto, Makoto; Suda, Michio; Shimokura, Masanori; Kiso, Yoshiaki; Kihara, Masahiro; Yamori, Yukio; Nishimura, Kazunobu; Soneda, J; Ban, Toshihiko; Imura, Hiroo

doi:10.1016/0006-291x(85)90170-6

Cited by 224 publications

(77 citation statements)

References 16 publications

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“…It not only exhibited the best displacement curve of antibody bound to 125 1 CDN 1-16 by unlabelled CDN [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] but also demonstrated the highest cross-reactivity with endogenous pro-ANP in both human and rat. As the complete endogenous molecule (pro-ANP was not available synthetically formal cross-reactivity studies could not be performed, but this antiserum could detect the highest concentrations of cardiodilatin-IR and exhibited the best correlation with ANP concentrations, determined by the ANP radioimmunoassay, in the same human atrial extracts.…”

Section: Antiseramentioning

confidence: 99%

“…As the complete endogenous molecule (pro-ANP was not available synthetically formal cross-reactivity studies could not be performed, but this antiserum could detect the highest concentrations of cardiodilatin-IR and exhibited the best correlation with ANP concentrations, determined by the ANP radioimmunoassay, in the same human atrial extracts. The cross-reactivity of the antiserum with human synthetic CDN [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] was 100% and with CDN 1-67 44%. The antiserum showed no cross-reactivity with alpha-human-ANP.…”

Section: Antiseramentioning

confidence: 99%

“…The iodinated CDN [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] was eluted from the HPLC column by a concentration of 30% (v j v) acetonitrile in water containing 0.05% (v j v) trifluoroacetic acid, in a single radioactivity peak (specific activity of 60-70 Bqj fmol). In the antibody-free tube, non-specific binding was < 3%, zero standard binding was 45-50% and excess antibody binding was 85-90%.…”

Section: Iodinationmentioning

confidence: 99%

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The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Meleagros

Ghatei

Anderson

et al. 1988

Clinica Chimica Acta

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A sensitive and specific radioimmunoassay has been developed for cardiodilatin; the N-terrninal peptide sequence of the atrial natriuretic peptide (ANP) prohorrnone. Cardiodilatin-immunoreactivity (-IR) concentrations in the human right atrial appendage we re found to correlate with ANP-IR concentrations, deterrnined by an established radioimmunoassay, (cardiodilatin-IR = 13.2 ± 1.2 nmoljg, ANP-IR = 19.8 ± 2.0 nmoljg, r = 0.80, p < 0.001). Characterisation of the cardiodilatin-IR in the human and rat right atrium by gel permeation and fast protein liquid chromatography revealed only two cardiodilatin-IR molecular forms. The larger more hydrophobic form, the majority of the cardiodÜatin-IR, contained in addition ANP-IR and therefore represents the prohorrnone. The smaller, less hydrophobic form, lacked ANP-IR and thus represents the cleaved N-terrninal peptide sequence of the prohorrnone. These findings indicate that the prohorrnone is the major molecular form in the human and rat atrium. Furthermore, they demonstrl:lte that a single large N-terrninal peptide, cardiodilatin, derived from the prohorrnone, may exist as a distinct molecular form in the atrium of these species.

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Section: Antiseramentioning

confidence: 99%

Section: Antiseramentioning

confidence: 99%

Section: Iodinationmentioning

confidence: 99%

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The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Meleagros

Ghatei

Anderson

et al. 1988

Clinica Chimica Acta

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“…human ANF-(99-126), 7>8 we previously demonstrated that human ANF-(99-126) of 28 amino acids is released from the heart and circulates in the body, 9 l0 that the bolus injection of synthetic human ANF-(99-126) into humans results in a rapid increase of natriuresis and diuresis and a rapid fall in blood pressure accompanied by an increase in heart rate, 6 and that the infusion of human ANF-(99-126) in patients with congestive heart failure improves cardiac performance." We also demonstrated the wide distribution of ANF in the brain by RIA and immunohistochemistry 8 ' n -n and its prevalence in the anteroventral third ventricular (AV3V) region, and we showed that brain ANF is involved in central cardiovascular control alone or in combination with the brain renin-angiotensin system.""…”

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confidence: 99%

Atrial natriuretic factor in essential hypertension and adrenal disorders.

et al. 1988

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AKIRA SUGAWARA, KAZUWA NAKAO, TSUYOSHI KONO, NARTTO MORD, TAKAYUKI YAMADA, HIROSHI ITOH, SHOZO SHIONO, YOSHIHIKO SATTO, MASASHI MUKOYAMA, HIROSHI ARAI, AND Hmoo IMURA SUMMARY Patients with untreated essential hypertension had significantly higher plasma atrial natriuretic factor (ANF) levels (92.9 ± 12.9 pg/ml, mean ± SE) than those of age-matched controls (37.8 ± 6.0 pg/ml; p<0.01). Plasma ANF levels in essential hypertensive patients showed a significant positive correlation with mean arterial pressure (MAP; r = 0.46, p<0.05) and an inverse correlation with plasma renin activity (PRA; r = -0.43, p<0.05). Plasma ANF levels after medication showed significant correlation with the decrease hi MAP (r = 0.565, p<0.05). Patients with primary aldosteronism had significantly higher plasma ANF levels (122.4 ± 30.2 pg/ml, a = 8) than those of controls (p<0.05). The levels returned to normal after extirpation of adrenal tumors. The response of plasma ANF levels in patients with primary aldosteronism to volume expansion with infusion of 2 L of physiological saline in 2 hours was greater than hi controls. Such exaggerated response disappeared after surgical treatment. Infusion of angiotensin II (Ang II; 20 ng/kg/min) or norepinephrine (200 ng/kg/min) for 30 minutes to normal volunteers (n = 5) resulted hi a rise hi MAP (24.9 ± 3.3 and 15.8 ± 4.4 mm Hg, respectively) and a twofold increase in plasma ANF level. Infusion of the Ang II antagonist [Sar l ,De r |Ang II (600 ng/kg/min) for 30 minutes, resulted hi a rise hi MAP (18.8 ± 2 . 1 mm Hg) and more than a twofold increase ui plasma ANF level in patients with essential hypertension (n = 6). The increase in the plasma ANF level by the agonistic pressor action of the Ang II antagonist significantly correlated with the increase in MAP (r = 0.760, p<0.05). These results suggest that the plasma ANF level is one of the important clinical parameters for evaluating the hemodynamic state hi essential hypertension, primary aldosteronism, and other adrenal disorders. (Hypertension 11 [Suppl I]: I-212-I-216, 1988)

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“…Atrial natriuretic polypeptide (ANP)l is a cardiac hormone secreted through the coronary sinus from the heart and is involved in fluid, electrolyte, and vascular homeostasis (1)(2)(3)(4)(5)(6)(7). The plasma ANP concentration is increased in patients with congestive heart failure (CHF) in relation to its severity (8)(9)(10), and this elevation of the plasma ANP concentration is mainly due to increased secretion of ANP from the heart (7, 1 1).…”

mentioning

confidence: 99%

Augmented expression of atrial natriuretic polypeptide gene in ventricle of human failing heart.

Saito¹,

Nakao²,

Arai³

et al. 1989

J. Clin. Invest.

232

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IntroductionTo elucidate the expression of the atrial natriuretic polypeptide (ANP) gene in the ventricle of the human failing heart, we have measured ANP and ANP messenger RNA (ANPmRNA) levels in left ventricular aneurysm obtained at operation, biopsy specimens of left ventricles from dilated cardiomyopathy (DCM) and autopsy samples of old myocardial infarction (OMI) and DCM hearts, and compared the levels with those in the normal ventricle. The ANP level (mean±SE) was 17.5±6.9 ng/g in the normal ventricle, and increased to 6603±122.2 ng/g in the left ventricular aneurysm tissues and to 3,138.6±1,642.1 ng/g in the biopsy specimens of the DCM ventricle. These levels were 40 and 200 times higher than in the normal ventricle. The increase of ANP levels was observed in both infarcted and noninfarcted regions of the OMI heart, and in the entire ventricle of the DCM heart. A significant positive correlation was found between the ANP level in aneurysm tissues and pulmonary capillary wedge pressure (r = 0.85). The ANPmRNA level in the left ventricular aneurysm showed about a 10-fold increase compared with that in the normal heart and reached 23% of that in the atrium of the same heart. A similar increase in the ANPmRNA level was observed in the entire ventricle of DCM. These data clearly indicate that the expression of the ANP gene in the ventricle is augmented in the failing heart in accordance with the severity of heart failure. In the atrium of the failing heart, ANP and ANPmRNA levels were only two times higher than those in the normal atrium. Thus, the augmentation in the expression of the ANP gene was more prominent in the ventricle than in the atrium. Taking tissue weight into account, the total content of ANPmRNA in the ventricle of the failing heart is much the same as that in the normal atrium. The ratio of the ANP level to the ANPmRNA level in the ventricle is much smaller than that in the atrium. These results suggest more rapid secretion of ANP after synthesis in the ventricle. These findings demonstrate that the expression of the ANP gene is augmented in the human ventricle of the failing heart and suggest that the ventricle becomes a substantial source of circulating ANP in congestive heart failure.Address reprint requests to Dr.

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α-Human atrial natriuretic polypeptide is released from the heart and circulates in the body

Cited by 224 publications

References 16 publications

The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Atrial natriuretic factor in essential hypertension and adrenal disorders.

Augmented expression of atrial natriuretic polypeptide gene in ventricle of human failing heart.

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