α-Glucosidase Inhibitory and Antioxidant Activities of DifferentIpomoea aquaticaCultivars and LC-MS/MS Profiling of the Active Cultivar

Abstract

The present study was designed to investigate the effect of Ipomoea aquatica extracted using water and methanol at various concentrations on the total phenolics, antioxidant capacity, and α‐glucosidase inhibitory activities. Three I. aquatica cultivars were used in this study including the upland type with narrow leaves (K‐11), low‐land aquatic types with broader shaped leaves (K‐25), and bamboo‐shaped leaves (K‐88). The results revealed that 70% methanol extract of K‐11 showed higher total phenolic content and α‐glucosidase inhibitory and antioxidant activities than the other two cultivars. The phytochemical constituents in the active extract K‐11 were analyzed by means of liquid chromatography coupled with diode array detection and electrospray tandem mass spectroscopy. Eighteen compounds were detected of which 13 were tentatively identified as quercetin‐3‐O‐sophoroside, quercetin‐3‐O‐glucoside, quercetin‐3,7‐di‐O‐glucoside, nomilinic acid glucoside, 4,5‐di‐O‐caffeoylquinic acid, 3,5‐di‐O‐caffeoylquinic acid, luteolin‐7‐glucoside and 3,4,5‐tricaffeolquinic acid, fatty acid together with quercetin, and tricaffeoylquinic acid derivatives. Practical Applications Antioxidants protect the human body against infections and degenerative diseases by inhibiting and scavenging free radicals. The present study showed that all methanol extracts of I. aquatica are rich in polyphenols in which 70% methanol extract showed highest in vitro antioxidant and α‐glucosidase inhibitory activities. Knowledge about the antioxidant and α‐glucosidase inhibitory activities of I. aquatica will promote its usage as a functional food, and it can be utilized as an antioxidant sources in food industry.

Identification of α-glucosidase inhibitors from Clinacanthus nutans leaf extract using liquid chromatography-mass spectrometry-based metabolomics and protein-ligand interaction with molecular docking

“…All fractions were prepared in six replications and were recovered and stored at − 80 °C prior to analysis [11], [12]. All fractions were tested using an α-glucosidase inhibitory activity assay and analyzed using the Q-TOF LC-MS instrument [10], [13].…”

“…LC-MS was used to detect the metabolites present in the leaf fractions from C. nutans , as described by Lawal et al [13] with slight modifications. The samples were analyzed using the Q-TOF LC-MS system, Agilent 1290 Infinity, and 6550 iFunnel Q-TOF LC/MS (Agilent Technologies, Santa Clara, California) equipped with an electrospray (ESI) interface comprising both positive and negative ion modes.…”

UHPLC-ESI-Orbitrap-MS Analysis of Biologically Active Extracts from Gynura procumbens (Lour.) Merr. and Cleome gynandra L. Leaves

“…Among the treatments available for diabetes, the inhibition of α-glucosidase is one of them, which is responsible for glucose breakdown in the intestinal wall [17]. Plants' phenolic compounds have been reported to have the ability in inhibiting this enzyme, yet more often, modern drugs can be prescribed to combat the occurrence of inflammation-related diseases and diabetes [18]. However, prolonged use of these drugs stimulates unwanted side effects towards the liver, kidney, and other organs [19].…”

Identification and Functional Characterization of Genes Involved in the Biosynthesis of Caffeoylquinic Acids in Sunflower (Helianthus annuus L.)

“…scolymus (globe artichoke; Moglia et al, 2016), and Helianthus annuus L. (sunflower; Sun et al, 2012) in the family Asteraceae; Solanum lycopersicum L. (tomato) and Nicotiana tabacum L. (tobacco) in the family Solanaceae (Niggeweg et al, 2004); Coffea spp. (coffee; Lallemand et al, 2012) in the family Rubiaceae; and Ipomoea batatas L. (sweet potato; Kojima and Kondo, 1985) and Ipomoea aquatica (water spinach; Lawal et al, 2016) in the family Convolvulaceae. The antioxidant, anti-inflammatory, anti-hypertension, and antimicrobial properties of CQAs are well documented by previous studies.…”