α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC–DAD

Kumar, Deepak; Gupta, Nidhi; Ghosh, Rina; Gaonkar, Raghuvir H.; Pal, Buddhadev

doi:10.1016/j.jff.2012.10.007

Cited by 55 publications

(35 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The α-amylase inhibition method was reported by Kumar et al 29 Acarbose was used as a positive control, while phosphate buffer (0.02 M PBS, pH 6.9) was used as a negative control in place of the sample. Each sample was tested in triplicate with different concentrations.…”

Section: Alpha-amylase Inhibition Activitymentioning

confidence: 99%

<i>In Vitro</i> Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts

Ergül¹,

Eruygur²,

Ataş³

et al. 2019

tjps

View full text Add to dashboard Cite

Bu çalışmada, Ficus carica yapraklarına ait su ve metanol ekstrelerinin diyabet ve Alzheimer hastalığı ile ilişkili enzimlerin inhibisyonu üzerine etkisinin araştırılması amaçlanmıştır. Ayrıca, ekstrelere ait biyoaktif bileşenler, antikanser, antioksidan ve antimikrobiyal etkiler de araştırılmıştır. Gereç ve Yöntemler: Ekstrelerdeki biyoaktif bileşikler gaz kromatografisi-kütle spektrometresi metodu ile belirlenmiştir. Antioksidan aktivite, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6 sulphonic acid) (ABTS) radikal süpürücü, toplam fenol ve flavonoid içeriği, ferrik indirgeme gücü ve demir şelasyon yöntemleriyle değerlendirilmiştir. Antikanser, antikolinesteraz ve antimikrobiyal etkinlikler ise sırasıyla XTT yöntemi, Ellman yöntemi ve mikrodilüsyon tekniği yöntemi ile belirlenmiştir. Bulgular: Elde ettiğimiz sonuçlar su ve metanol ekstreleri arasında kimyasal bileşim açısından farklılık olduğunu ve her iki ekstrenin de güçlü antioksidan aktiviteye sahip olduğunu göstermiştir. Benzer şekilde, her iki ekstrede güçlü α-glikozidaz ve α-amilaz aktivite gösterirken, su ekstresi metanole göre daha güçlü asetilkolinesteraz ve butirilkolinesteraz inhibisyon etkiye sahiptir. F. carica metanol ekstresi MDA-MB-231 hücreleri üzerinde güçlü antikanser etki, Escherichia coli ve Staphylococcus aureus'e karşı ise orta düzeyde antimikrobiyal etki göstermiştir. Sonuç: Bulgularımız, F. carica yapraklarının kanser, diyabet ve Alzheimer hastalığında umut verici bir terapötik ajan geliştirmek için değerli bir kaynak olabileceğini düşündürmektedir.

show abstract

Section: Alpha-amylase Inhibition Activitymentioning

confidence: 99%

<i>In Vitro</i> Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts

Ergül¹,

Eruygur²,

Ataş³

et al. 2019

tjps

View full text Add to dashboard Cite

show abstract

“…α-Glucosidase inhibitory potency was measured according to a reported method (Kumar et al, 2013). Acarbose was used as positive control and the uninhibited enzyme was taken as negative control (DMSO control).…”

Section: α-Glucosidase Inhibitory Assaymentioning

confidence: 99%

Anti-diabetic potential of selected ethno-medicinal plants of north east India

Sheikh

Maibam

Biswas

et al. 2015

Journal of Ethnopharmacology

View full text Add to dashboard Cite

“…α-Amylase is in a class of enzymes that hydrolyze polysaccharides to oligosaccharides, while α-glucosidase catalyzes the final step in carbohydrate hydrolysis to release the absorbable monosaccharides. [2] Both of these carbohydrate-hydrolyzing enzymes are secreted in the small intestine, while α-amylase is also found in the saliva. [1] Thus, the inhibition of these enzymes plays an important role in the management of diabetic complications, particularly type 2 diabetes.…”

Section: Introductionmentioning

confidence: 99%

“…[1] Thus, the inhibition of these enzymes plays an important role in the management of diabetic complications, particularly type 2 diabetes. [2,3] Antidiabetic drugs such as acarbose, miglitol and voglibose act by inhibiting the activities of α-amylase and α-glucosidase. Although these drugs are effective, continuous use may lead to undesirable side effects, which include liver toxicity and adverse gastrointestinal symptoms.…”

Section: Introductionmentioning

confidence: 99%

“…A large number of traditional medicinal plants and plant-derived constituents, such as terpenes, alkaloids, curcuminoids, anthocyanins, flavonoids, quinones, phenols, phenylpropanoids, acylphenols and dimeric acylphenols, are known to exhibit α-amylase and αglucosidase inhibitory activities. [2,[5][6][7][8] However, to date, bis-labdanic diterpenes have never been investigated for their potential to inhibit these enzymes. Therefore, herein, we report the dual inhibiting potentials of pahangensin A (1; Figure 1), a bislabdanic diterpene previously isolated and characterized from the rhizomes of Alpinia pahangensis RIDL., against α-amylase and α-glucosidase.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Insight and Mode of Inhibition of α‐Glucosidase and α‐Amylase by Pahangensin A from Alpinia pahangensisRidl.

Loo

Leong

Sivasothy

et al. 2019

Chemistry & Biodiversity

View full text Add to dashboard Cite

The inhibition of carbohydrate‐hydrolyzing enzymes in human digestive organs is crucial in controlling blood sugar levels, which is important in treating type 2 diabetes. In the current study, pahangensin A (1), a bis‐labdanic diterpene characterized previously in the rhizomes of Alpinia pahangensis Ridl., was identified as an active dual inhibitor for α‐amylase (IC50=114.80 μm) and α‐glucosidase (IC50=153.87 μm). This is the first report on the dual α‐amylase and α‐glucosidase inhibitory activities of a bis‐labdanic diterpene. The Lineweaver‐Burk plots of compound 1 indicate that it is a mixed‐type inhibitor with regard to both enzymes. Based on molecular docking studies, compound 1 docked in a non‐active site of both enzymes. The dual inhibitory activity of compound 1 makes it a suitable natural alternative in the treatment of type 2 diabetes.

show abstract

α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC–DAD

Cited by 55 publications

References 46 publications

<i>In Vitro</i> Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts

<i>In Vitro</i> Evaluation of the Chemical Composition and Various Biological Activities of Ficus carica Leaf Extracts

Anti-diabetic potential of selected ethno-medicinal plants of north east India

Molecular Insight and Mode of Inhibition of α‐Glucosidase and α‐Amylase by Pahangensin A from Alpinia pahangensisRidl.

Contact Info

Product

Resources

About