α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.)

Bhandari, Megh Raj; Jong-Anurakkun, Nilubon; Gao, Hong; Kawabata, Jun

doi:10.1016/j.foodchem.2007.05.077

Cited by 403 publications

(255 citation statements)

References 26 publications

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“…One therapeutic approach for treating diabetes is decreasing post-prandial hyperglycemia by delaying glucose absorption through carbohydrate-hydrolyzing enzymes inhibition, α-glucosidase and α-amylase in the digestive tract. Inhibitors of these enzyme delay carbohydrate digestion and prolong overall carbohydrate digestion time, causing a reduction in the rate of glucose absorption and consequently blunting the postprandial plasma glucose rise (Rhabasa-Lhoret and Chiasson, 2004;Bhandari et al, 2008;Ban et al, 2009). Recently, considerable interest has been generated by a novel class of antihyperglycemic agents that act at distinct levels of the incretin pathway.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Elya¹,

Handayani²,

Sauriasari³

et al. 2015

Pakistan J. of Biological Sciences

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A B S T R A C T Active compounds of plants have potency as antidiabetic that can be used in diabetes mellitus treatment. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase and preventing the hydrolysis of Glucagon Like Peptide-1 (GLP-1) through inhibition of Dipeptidyl peptidase IV. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of α-glucosidase, α-amylase and Dipeptidyl peptidase IV from 42 ethanolic extracts of selected Indonesian plants. Inhibitory activity was measured using spectrophotometric method. α-amylase activity was measured at λ = 540 nm, α-glucosidase activity and the DPP-IV activity were measured at λ = 405 nm. Eight extracts inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. These extracts are Camelia sinensis (white, green, fermented), Pometia pinnata, Syzygium polyanthum, Artocarpus heteropyllus, Lagerstroemia speciosa and Persea Americana. The ethanolic extracts have DPP-IV inhibitory activity between 10-20%, lower than Diprotin as positive control, except Camelia sinensis (white) which gave the inhibition more than 30% at 62.5 µg mLG 1 . This study showed us that some extracts have inhibition activity of α-glucosidase, α-amylase and DPP-IV.

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Section: Introductionmentioning

confidence: 99%

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Elya¹,

Handayani²,

Sauriasari³

et al. 2015

Pakistan J. of Biological Sciences

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“…Therefore, many studies have been conducted to find effective inhibitors of α-glucosidase and α-amylase from natural materials and to develop physiological functional food for treating DM [7] [25]. Our results show that inhibition ability of hot water extract (HWGT) for α-glucosidase from yeast were better than that of rat small intestine (p = 0.013).…”

Section: Discussionmentioning

confidence: 81%

Hypoglycemic and Antioxidative Effects of <i>Glossogyne tenuifolia</i> on Streptozotocin-Nicotinamide-Induced Diabetic Rats

Wang¹,

Huang²,

Hsu³

et al. 2017

AJPS

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Glossogyne tenuifolia (GT) is the traditional herbal tea in Penghu Island, Taiwan. Recent research has shown that the active components in GT are potential inhibitors of α-glucosidase. The present study investigated that whether or not GT could improve the status of type 2 diabetes mellitus. Male Wistar rats aged eight weeks were induced to be hyperglycemic by subcutaneous injection of streptozotocin-nicotinamide (STZ-NA) and combination of high-fat diet (HFD). The animals were given GT extracts at the low dose or high dose, or the anti-diabetic drug (acarbose), in drinking water for 4 weeks. The results showed that hot water extracts from GT resulted in significantly decreases in fasting blood glucose at the 1 st and 2 nd weeks, fasting insulin levels at the 2 nd week, 1 hour postprandial blood glucose after the starch tolerance test on Day 23 and blood glucose levels after oral glucose tolerance test (OGTT) at the 60 th minute on Day 25. In addition, diabetic rats treated with GT extracts from hot water for 4 weeks displayed significantly decreased thiobarbituric acid reactive substances (TBARS) in the serum, liver and kidney, serum total cholesterol, fasting insulin levels and homeostasis model assessment for insulin resistance (HOMA-IR). Overall, these results demonstrate that the hot water extracts of GT might improve the progression of diabetes and decrease oxidative stress in STZ-NA-induced diabetic rats.

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“…In the present study the TCME shown significantα-glucosidase and α-amylase inhibitory activity but it is unclear whether the mechanism of inhibition of α-amylase and α-glucosidase by TCME is due to the competitive and noncompetitive method. The fact that α-amylase and α-glucosidase showed different inhibition kinetics seemed to be due to structural differences related to the origins of the enzymes [35]. However, the inhibition rate for α-glucosidase was close to that of acarbose, but the inhibition rate for α-amylase was obviously lower than that of nojirimycine.…”

Section: Discussionmentioning

confidence: 96%

“…Skeletal muscle represents 30-40% of the total body weight and seems to be one of the most important target tissues for the action of insulin and for the uptake of glucose at the peripheral level [37]. It is a well-known fact that insulin and antidiabetic drugs promote glucose uptake by peripheral cells and tissues [35]. Another important finding of this work is that TCME possesses considerable insulin-like properties, as evidenced by the enhancement of glucose uptake in the diaphragm, which represents muscle cells that are a major site of insulin-mediated glucose disposal.…”

Section: Discussionmentioning

confidence: 99%

In Vivo and in Vitro Evaluation of Tephrosia Calophylla for Anti-Diabetic Properties

A²

2018

Int J Pharm Pharm Sci

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Objective:The objective the present work was to investigate in vivo and in vitro anti-diabetic potentials of methanol extract of Tephrosia calophylla against alloxan-induced diabetes in albino rats. Methods:For in vivo evaluation, diabetes was induced in albino rats by administering a single dose of alloxan. The study was designed to test the acute effect of methanol extract of Tephrosia calophylla (TCME) to reduce blood glucose in OGTT. The chronic study of 21 d was performed against diabetic rats and blood glucose was determined at 1 st , 7 th , 14 th and 21 st day. In chronic in vivo study, serum concentrations of insulin, urea, creatinine, total cholesterol, triglycerides, ALT and AST were also estimated at 21 st day. The in vitro α-glucisidase inhibitory activity and α-amylase inhibitory activity were performed and IC50Results: In OGTT, standard glibenclamide and TCME at 400 mg/kg treated animals have shown significant reduction in blood glucose at 90 min but at 120 min, blood glucose level (BGL) was significantly reduced in glibenclamide and TCME at 200 mg/kg and 400 mg/kg treated animals compared to diabetic control group. In chronic modelthe methanol extract effectively reduced blood glucose levels (P<0.001) at 14 th and 21 values of the extract was determined. The glucose uptake by rat hemidiaphragm model was also used test potentials of the extract to increase utilization of the glucose by tissues.st day of study in therapeutic groups and effect was comparable to that of standard. The extract could also significantly (P<0.001) reduce concentrations of SGOT, triglycerides (TGs), Total cholesterol (TC) and urea in serum and significantly (P<0.001) increased the insulin level in blood which proves beneficial effects of the extract in diabetes. The change in concentrations of SGPT and urea were less significant (P>0.01). In vitro studies, against both α −glucosidae and α − amyalase inhibitory activities, extract has shown significant IC50 Conclusion:The results obtained from the present study suggest that, the methanol extract of Tephrosia calophylla possess significant in vivo antidiabetic properties against alloxan induced diabetes in rats. The results also suggests that, TCME also possess the significant in vitro anti-dabetic potentials.values and it also enhanced glucose utilization by rat hemidaphragm.

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α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.)

Cited by 403 publications

References 26 publications

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Hypoglycemic and Antioxidative Effects of <i>Glossogyne tenuifolia</i> on Streptozotocin-Nicotinamide-Induced Diabetic Rats

In Vivo and in Vitro Evaluation of Tephrosia Calophylla for Anti-Diabetic Properties

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α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.)

Cited by 403 publications

References 26 publications

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Hypoglycemic and Antioxidative Effects of &lt;i&gt;Glossogyne tenuifolia&lt;/i&gt; on Streptozotocin-Nicotinamide-Induced Diabetic Rats

In Vivo and in Vitro Evaluation of Tephrosia Calophylla for Anti-Diabetic Properties

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Hypoglycemic and Antioxidative Effects of <i>Glossogyne tenuifolia</i> on Streptozotocin-Nicotinamide-Induced Diabetic Rats