α-Glucosidase and α-Amylase Inhibitors from <i>Arcytophyllum thymifolium</i>

Milella, Luigi; Milazzo, Stella; Leo, Marinella De; Saltos, Mariela Beatriz Vera; Faraone, Immacolata; Tuccinardi, Tiziano; Lapillo, Margherita; Tommasi, Nunziatina De; Braca, Alessandra

doi:10.1021/acs.jnatprod.6b00484

Cited by 81 publications

(47 citation statements)

References 44 publications

(80 reference statements)

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“…Hypoglycemic activity of CE and AF was tested by evaluating their inhibitory activity against α-glucosidase and α-amylase, two of the main enzymes involved in postprandial hyperglycemia modulation [41]. AF represents 28% (g/g dry CE) and this recovery agreed with the range of data reported in literature by Lee et al [5] and Lao et al [1] for other purple corn cob varieties.…”

Section: Evaluation Of Hypoglycemic Activitysupporting

confidence: 82%

“…The α-amylase inhibition assay was performed following the method reported by Milella et al [41] with some modifications. Briefly, 500 µL of Type VI-B porcine pancreatic α-amylase (0.5 mg/mL in 100 mM sodium phosphate buffer, containing 6.7 mM sodium chloride, pH 6.7-PBS) were preincubated with 500 µL of each sample (CE or AF) in test tubes at 37 • C for 10 min.…”

Section: α-Amylase Inhibitory Activitymentioning

confidence: 99%

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A New Italian Purple Corn Variety (Moradyn) Byproduct Extract: Antiglycative and Hypoglycemic In Vitro Activities and Preliminary Bioaccessibility Studies

et al. 2020

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The reuse of byproducts from agricultural and food industries represents the key factor in a circular economy, whose interest has grown in the last two decades. Thus, the extraction of bioactives from agro-industrial byproducts is a potential source of valuable molecules. The aim of this work was to investigate the in vitro capacity of byproducts from a new Italian corn variety, named Moradyn, to inhibit the accumulation of advanced glycation end products (AGEs) involved in several chronic age-related disorders. In addition, the hypoglycemic effect of Moradyn was tested by in vitro enzymatic systems. A Moradyn phytocomplex and its purified anthocyanin fraction were able to inhibit fructosamine formation and exhibited antiglycative properties when tested using BSA-sugars and BSA-methylglyoxal assays. These properties could be attributed to the polyphenols, mainly anthocyanins and flavonols, detected by RP-HPLC-DAD-ESI-MS n . Finally, a Moradyn phytocomplex was submitted to a simulated in vitro digestion process to study its bioaccessibility. Moradyn could be considered as a promising food ingredient in the context of typical type 2 diabetes risk factors and the study will continue in the optimization of the ideal formulation to preserve its bioactivities from digestion. Molecules 2020, 25, 1958 2 of 21 pelargonidin-3,5-di-O-glucoside [3]; cyanidin-, peonidin-, and perlargonidin-3-O-di-malonylglucoside [9], cyanidin-3-O-succinylglucoside, cyanidin-3-O-malonylglucoside-5-O-glucoside [5], and cyanidin-3,5di-O-glucoside, this one identified for the first time in purple corn by Žilić et al. [10]. Moreover, several authors have detected ethyl-malonylated anthocyanins that probably originate during the extraction process, and which are known to stabilize pigments, thus prolonging shelf life [5,8].Husk and cob extracts are richer in anthocyanins than kernel extracts, ranging from 0.49% to 4.6% (dry weight) for cobs [1,5]; moreover, husks and cobs differ from kernels for the presence of perlagonidin in their phytocomplex [3].Nowadays, several food byproducts such as olive leaves and oil mill wastewater, grape skin, fruit and vegetable seeds, and artichoke bracts and stems have been deeply investigated for their important role in the inhibition of advanced glycation end products (AGEs) formation [11][12][13][14][15]. AGEs are typical adducts derived from Maillard reaction or non-enzymatic glycation process. These compounds are involved in many chronic diseases, such as cardiovascular pathologies, type 2 diabetes, and neurodegenerative disorders, and their formation increases in association with hyperglycemia and metabolic dysfunctions [16][17][18][19]. Thanks to their high polyphenol content, purple corn byproducts have been associated with several health properties such as antioxidant, anti-inflammatory, and antiglycative activity [2,10,[20][21][22][23]. Moreover, in vitro and in vivo studies demonstrate their capacity in the prevention of cardiovascular and type 2 diabetes risk factors by exerting a strong anti-hypertensi...

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Section: Evaluation Of Hypoglycemic Activitysupporting

confidence: 82%

Section: α-Amylase Inhibitory Activitymentioning

confidence: 99%

A New Italian Purple Corn Variety (Moradyn) Byproduct Extract: Antiglycative and Hypoglycemic In Vitro Activities and Preliminary Bioaccessibility Studies

et al. 2020

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“…In this study, it was also observed that the concentration of flavonoids of A. indica was significantly lower when compared with that of P. nigrescens at P<0.05. As natural antioxidants, flavonoids play an important role in scavenging free radicals and preventing degenerative diseases such as cardiovascular diseases [58,59,60]. However, they are also involved in the antiproliferation of carcinogenic cells, in cell cycle regulation and in the induction of apoptosis [61,62,63].…”

Section: Resultsmentioning

confidence: 99%

Comparative Assessment of Phytochemical Content and Antioxidant Potential of Azadirachta indica and Parquetina nigrescens Leaves

Airaodion

Olatoyinbo²,

Ogbuagu

et al. 2019

APRJ

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Aim: The aim of this study is to compare the phytochemical content and antioxidant potential of Azadirachta indica and Parquetina nigrescens leaves. Study Design: This study was made to fit a one way Analysis of Variance. Place and Duration of Study: This research was carried out in Premedical Science Department, Educational Advancement Centre, Ibadan and Pharmaceutical Laboratory of the University of Ibadan, Nigeria between January and June, 2018. Methodology: Both plants were harvested from the botanical garden, University of Ibadan. The qualitative and quantitative analyses as well as antioxidant potential of both plants were investigated. Results: The result of the qualitative analysis showed that both plants contained variety of phytochemicals. The quantitative analyses showed that these phytochemicals were present in different concentrations. The concentration of phytate and total phenolics were significantly higher in A. indica when compared with those of P. nigrescens respectively at P<0.05. It was also observed that A. indica had lower concentrations in alkaloids, saponin, flavonoids and tannin when compared with those of P. nigrescens respectively. Also tested were antioxidants (ascorbic acid, DPPH and FRAP). The concentration of ascorbic acid was significantly higher in A. indica when compared with that of P. nigrescens at P<0.05. α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging potential of A. indica and P. nigrescens was investigated respectively at different concentrations with A. indica having the higher radical scavenging potential. The scavenging potential of DPPH was found to increase with increasing concentration of the extracts. Conclusion: Result of this study showed that both plants are rich in phytochemicals and possess antioxidant potential. Hence, they might act as prophylactic and remedy for different diseases, such as cancer, atherosclerosis, obesity, etc. Parquetina nigrescens might be more potent than Azadirachta indica in acting as a remedy for different diseases.

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“…Optical rotations were measured on an Atago AP-300 digital polarimeter equipped with a sodium lamp (589 nm) and a 1-dm microcell. NMR experiments were recorded at 300 K in CD 3 OD on a Bruker DRX-600 spectrometer (Bruker BioSpin GmBH) equipped with a Bruker 5 mm TCI CryoProbe as reported previously [26]. HRESIMS were acquired in the positive ion mode on a LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific).…”

Section: General Experimental Proceduresmentioning

confidence: 99%

New Phenylethanoid Glycosides from Cistanche phelypaea and Their Activity as Inhibitors of Monoacylglycerol Lipase (MAGL)

et al. 2018

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Four new phenylethanoid glycosides (1: -4: ), 1--hydroxyphenyl-ethyl-2--acetyl-3,6-di--l-rhamnopyranosyl--d-glucopyranoside (1: ), 1---hydroxyphenyl-ethyl-3,6--di--l-rhamnopyranosyl--d-glucopyranoside (2: ), 1---hydroxyphenyl-ethyl-2--acetyl-3,6-di--l-rhamnopyranosyl-4--coumaroyl--d-glucopyranoside (3: ), and 1---hydroxyphenyl-ethyl-3,6-di--l-rhamnopyranosyl-4--coumaroyl--d-glucopyranoside (4: ), together with three known compounds, were isolated from the -butanol extract of aerial parts. The structural characterization of all compounds was performed by spectroscopic analyses, including 1D and 2D NMR, and HRESIMS experiments. The isolated compounds were assayed for their inhibitory activity on two enzymes involved in the peculiar glycolytic or lipidic metabolism of cancer cells, human lactate dehydrogenase (LDH), and monoacylglycerol lipase (MAGL), respectively. All the compounds showed negligible activity on LDH, whereas some of them displayed a certain inhibition activity on MAGL. In particular, compound 1: was the most active on MAGL, showing an IC value of 88.0 µM, and modeling studies rationalized the supposed binding mode of 1: in the MAGL active site.

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α-Glucosidase and α-Amylase Inhibitors from Arcytophyllum thymifolium

Cited by 81 publications

References 44 publications

A New Italian Purple Corn Variety (Moradyn) Byproduct Extract: Antiglycative and Hypoglycemic In Vitro Activities and Preliminary Bioaccessibility Studies

A New Italian Purple Corn Variety (Moradyn) Byproduct Extract: Antiglycative and Hypoglycemic In Vitro Activities and Preliminary Bioaccessibility Studies

Comparative Assessment of Phytochemical Content and Antioxidant Potential of Azadirachta indica and Parquetina nigrescens Leaves

New Phenylethanoid Glycosides from Cistanche phelypaea and Their Activity as Inhibitors of Monoacylglycerol Lipase (MAGL)

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