2014
DOI: 10.1099/mic.0.073171-0
|View full text |Cite
|
Sign up to set email alerts
|

α-fur, an antisense RNA gene to fur in the extreme acidophile Acidithiobacillus ferrooxidans

Abstract: A large non-coding RNA, termed a-Fur, of~1000 nt has been detected in the extreme acidophile Acidithiobacillus ferrooxidans encoded on the antisense strand to the iron-responsive master regulator fur (ferric uptake regulator) gene. A promoter for a-fur was predicted bioinformatically and validated using gene fusion experiments. The promoter is situated within the coding region and in the same sense as proB, potentially encoding a glutamate 5-kinase. The 39 termination site of the a-fur transcript was determine… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

0
3
0

Year Published

2016
2016
2020
2020

Publication Types

Select...
3

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(3 citation statements)
references
References 74 publications
0
3
0
Order By: Relevance
“…Under oxidative stress, mimicked by hydrogen peroxide or methyl viologen, a high expression of α-fur, which was associated with an activation of fur transcription, lead to a global increase of total Fur protein in M. aeruginosa PCC 7806 [36] in order to prevent hydroxyl radical formation. An antisense to a fur mRNA was also found in the extreme acidophile Acidithiobacillus ferrooxidans [37], an organism which lives in iron-rich conditions and which uses iron as an energy source. During iron depletion and accumulation of sulfur, both the sense and the antisense of fur were induced, possibly due to opening of a secondary structure on the RBS of the fur transcript, which allows efficient translation.…”
Section: Iron Homeostasismentioning
confidence: 97%
“…Under oxidative stress, mimicked by hydrogen peroxide or methyl viologen, a high expression of α-fur, which was associated with an activation of fur transcription, lead to a global increase of total Fur protein in M. aeruginosa PCC 7806 [36] in order to prevent hydroxyl radical formation. An antisense to a fur mRNA was also found in the extreme acidophile Acidithiobacillus ferrooxidans [37], an organism which lives in iron-rich conditions and which uses iron as an energy source. During iron depletion and accumulation of sulfur, both the sense and the antisense of fur were induced, possibly due to opening of a secondary structure on the RBS of the fur transcript, which allows efficient translation.…”
Section: Iron Homeostasismentioning
confidence: 97%
“…PCC 7120, Synechocystis sp. PCC 6803, Microcystis aeruginosa and in the proteobacteria Acidithiobacillus ferrooxidans [33][34][35][36][37]. This asRNA named α-fur is encoded in the 3′ untranslated region (UTR) of the fur gene and is involved in the repression of fur expression by an unknown mechanism.…”
Section: Metal Ion Homeostasismentioning
confidence: 99%
“…Strikingly, the α-fur promoters, located downstream of their respective fur genes, are not conserved and are likely to have emerged independently. Therefore, this suggests a strong evolutionary pressure toward asRNA-mediated regulation of Fur expression; possibly due to the ability of asRNAs to modulate their function according to intracellular Fe 2+ concentrations in iron-limited environments such as ocean for cyanobacteria and V. anguillarum or saturated in iron in the case of A. ferrooxidans, which is found in acidic water outflow from metal mines [37].…”
Section: Metal Ion Homeostasismentioning
confidence: 99%