α‐FETOPROTEIN (AFP) AND α<sub>2</sub>‐MACROGLOBULIN (α<sub>2</sub>‐M) AS THE MARKERS OF DISTINCT RESPONSES OF HEPATOCYTES TO CARCINOGENS IN THE RAT: CARCINOGENESIS

Dolezalová, V; Stratil, Pavel; Šimíčková, Marta; Kocent, A; Němeček, Radim

doi:10.1111/j.1749-6632.1983.tb32873.x

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Additionally, interleukin-1 and tumor necrosis factor-␣ mediate hepatocyte DNA synthesis and suppression of apoptosis induced by peroxisome proliferators, 38 suggesting that ␣ 2 M can also exert an influence by up-regulating some growth factors and cytokines such as interleukin-1 and tumor necrosis factor-␣. Several investigators 32,39 reported no elevations of serum ␣ 2 M level during rat hepatocarcinogenesis induced by NDEN, acetylaminofluorene, and 3Ј-methyl-4-dimethyl aminoazobenzene except at the late stage. We consider that this is probably because most of all lesions induced by these agents were GST-P-positive lesions.…”

Section: Discussionmentioning

confidence: 97%

α2-Macroglobulin: A Novel Cytochemical Marker Characterizing Preneoplastic and Neoplastic Rat Liver Lesions Negative for Hitherto Established Cytochemical Markers

Sukata

Uwagawa

Ozaki

et al. 2004

The American Journal of Pathology

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We tried to identify a novel marker characteristic for rat hepatocellular preneoplastic and neoplastic lesions, undetectable by well established cytochemical markers. Glutathione S-transferase placental (GST-P)-negative hepatocellular altered foci (HAF), hepatocellular adenoma (HCA), and hepatocellular carcinoma (HCC) were generated by two initiation-promotion models with N-nitrosodiethylamine (NDEN) and peroxisome proliferators, Wy-14,643 and clofibrate. Total RNAs isolated from laser-microdissected GST-P-negative HAF (amphophilic cell foci) and adjacent normal tissues were applied to microarray analysis. As a result, five up-regulated genes were identified, and further detailed examinations of the gene demonstrating most fluctuation, ie, that for alpha(2)-macroglobulin (alpha(2)M) were performed. In reverse transcriptase-polymerase chain reaction, alpha(2)M mRNA was overexpressed not only in amphophilic GST-P-negative HAF but also in amphophilic GST-P-negative HCA and HCC. In situ hybridization showed accumulation of alpha(2)M mRNA to be evenly distributed within GST-P-negative HAF (predominantly amphophilic cell foci). Distinctive immunohistochemical staining for alpha(2)M could be consistently demonstrated in GST-P-negative HAF, HCA, and HCC induced not only by peroxisome proliferators but also N-nitrosodiethylamine alone. Thus our findings suggest that alpha(2)M is an important novel cytochemical marker to identify hepatocellular preneoplastic and neoplastic lesions, particularly amphophilic cell foci, undetectable by established cytochemical markers and is tightly linked to rat hepatocarcinogenesis.

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Section: Discussionmentioning

confidence: 97%

α2-Macroglobulin: A Novel Cytochemical Marker Characterizing Preneoplastic and Neoplastic Rat Liver Lesions Negative for Hitherto Established Cytochemical Markers

Sukata

Uwagawa

Ozaki

et al. 2004

The American Journal of Pathology

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“…In contrast, although α-2-macroglobulin was more significantly different protein in this study, it had been reported already as a novel cytochemical not serological marker to identify rat hepatocellular preneoplastic and neoplastic lesions3233. So we chose MAL-associated Gal-3BP for further analysis on serum samples from HCC patients and healthy controls.…”

Section: Discussionmentioning

confidence: 71%

Discovering potential serological biomarker for chronic Hepatitis B Virus-related hepatocellular carcinoma in Chinese population by MAL-associated serum glycoproteomics analysis

Liu

et al. 2017

Sci Rep

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The accuracy of current biomarkers for the diagnosis of hepatocellular carcinoma (HCC), especially chronic Hepatitis B Virus (HBV)-related HCC, is limited. Recent progress in glycoproteomics has provided a novel platform for screening novel serological biomarkers of HCC. In this study, lectin affinity chromatography by Maackia amurensis lectin (MAL) and iTRAQ combined with mass spectrometric analysis were performed to enrich and identify the glycoprotein fractions in serum samples from HBV-related HCC patients and from healthy controls. Seventeen differential MAL-associated glycoproteins were identified. Among them, Galectin 3 binding protein (Gal-3BP) was selected for further evaluated by ELISA analysis and showed a high diagnostic potential of HBV-related HCC, with the AUC of 0.898 and a sensitivity, specificity and accuracy of 80.00%, 93.75% and 86.88%, respectively. Moreover, we constructed a predictive model through the combined use of serum Gal-3BP and Alpha Fetoprotein (AFP), which improved the sensitivity (from 87.5% to 95%), specificity (from 93.75% to 95%) and accuracy (from 90.63% to 95%) of diagnosing early HCC. These data suggested serum Gal-3BP level is a promising biomarker to identify HBV-related HCC and the combined use of serum Gal-3BP and AFP improves the diagnostic potential of HBV-HCC compared with AFP alone in current clinical practice.

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α‐FETOPROTEIN (AFP) AND α₂‐MACROGLOBULIN (α₂‐M) AS THE MARKERS OF DISTINCT RESPONSES OF HEPATOCYTES TO CARCINOGENS IN THE RAT: CARCINOGENESIS

Cited by 2 publications

References 7 publications

α2-Macroglobulin: A Novel Cytochemical Marker Characterizing Preneoplastic and Neoplastic Rat Liver Lesions Negative for Hitherto Established Cytochemical Markers

α2-Macroglobulin: A Novel Cytochemical Marker Characterizing Preneoplastic and Neoplastic Rat Liver Lesions Negative for Hitherto Established Cytochemical Markers

Discovering potential serological biomarker for chronic Hepatitis B Virus-related hepatocellular carcinoma in Chinese population by MAL-associated serum glycoproteomics analysis

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α‐FETOPROTEIN (AFP) AND α2‐MACROGLOBULIN (α2‐M) AS THE MARKERS OF DISTINCT RESPONSES OF HEPATOCYTES TO CARCINOGENS IN THE RAT: CARCINOGENESIS

Cited by 2 publications

References 7 publications

α2-Macroglobulin: A Novel Cytochemical Marker Characterizing Preneoplastic and Neoplastic Rat Liver Lesions Negative for Hitherto Established Cytochemical Markers

α2-Macroglobulin: A Novel Cytochemical Marker Characterizing Preneoplastic and Neoplastic Rat Liver Lesions Negative for Hitherto Established Cytochemical Markers

Discovering potential serological biomarker for chronic Hepatitis B Virus-related hepatocellular carcinoma in Chinese population by MAL-associated serum glycoproteomics analysis

Contact Info

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α‐FETOPROTEIN (AFP) AND α₂‐MACROGLOBULIN (α₂‐M) AS THE MARKERS OF DISTINCT RESPONSES OF HEPATOCYTES TO CARCINOGENS IN THE RAT: CARCINOGENESIS