α-Amylase production by Tepidimonas fonticaldi strain HB23: statistical optimization and compatibility study for use in detergent formulations

Allala, Fawzi; Bouacem, Khelifa; Boucherba, Nawel; Mechri, Sondes; Kriaa, Mouna; Arkoub-Djoudi, Warda; Azzouz, Zahra; Benallaoua, Saı̈d; Hacène, Hocine; Jaouadi, Bassem; Bouanane‐Darenfed, Amel

doi:10.1007/s11356-020-10142-2

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Therefore, it may be concluded that BBD-based RSM strategies can significantly enhance xylanase production. The effect of the optimization method is shown by the increased enzyme production and also by the decrease in enzyme production cost, which is mostly based on the use of inexpensive and easily accessible complex substrates like wheat straw and bran [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

et al. 2021

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Plant biomass constitutes the main source of renewable carbon on the planet. Its valorization has traditionally been focused on the use of cellulose, although hemicellulose is the second most abundant group of polysaccharides on Earth. The main enzymes involved in plant biomass degradation are glycosyl hydrolases, and filamentous fungi are good producers of these enzymes. In this study, a new strain of Aspergillus niger was used for hemicellulase production under solid-state fermentation using wheat straw as single-carbon source. Physicochemical parameters for the production of an endoxylanase were optimized by using a One-Factor-at-a-Time (OFAT) approach and response surface methodology (RSM). Maximum xylanase yield after RSM optimization was increased 3-fold, and 1.41- fold purification was achieved after ultrafiltration and ion-exchange chromatography, with about 6.2% yield. The highest activity of the purified xylanase was observed at 50 °C and pH 6. The enzyme displayed high thermal and pH stability, with more than 90% residual activity between pH 3.0–9.0 and between 30–40 °C, after 24 h of incubation, with half-lives of 30 min at 50 and 60 °C. The enzyme was mostly active against wheat arabinoxylan, and its kinetic parameters were analyzed (Km = 26.06 mg·mL−1 and Vmax = 5.647 U·mg−1). Wheat straw xylan hydrolysis with the purified β-1,4 endoxylanase showed that it was able to release xylooligosaccharides, making it suitable for different applications in food technology.

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Section: Resultsmentioning

confidence: 99%

Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

et al. 2021

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“…Several reports exist of successful RSM application, with methodologies like the Box-Behnken design, Plackett-Burhman factorial design, and central composite design (CCD), for process parameter optimization and attainment of enhanced enzyme production ([ [34] , [39] , [40] , [41] ]; Paul et al., 2021). RSM has been applied successfully for enhanced alpha-amylase production [ [4] , [42] , [43] , [44] , [45] , [46] ].…”

Section: Introductionmentioning

confidence: 99%

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

Ugwuoji

Nwagu

Ezeogu

2023

Biotechnology Reports

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“…These enzymes catalyze the reactions at low temperatures, reduce the release of harmful components to the environment, and enhance the ability to removing the harsh stains and improve the cloth softness, whiteness, and color (Al-ghanayem, 2020). Numerous examples can be cited from the literature indicating the high potential of alkaline, detergent-compatible α-amylases as an environmentally friendly component in stain removal and brightness of fabric (Hmidet et al, 2019;Allala et al, 2020;Kherouf et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient Computationally Derived Novel Metagenome α-Amylase With Robust Stability Under Extreme Denaturing Conditions

et al. 2021

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α-Amylases are among the very critical enzymes used for different industrial purposes. Most α-amylases cannot accomplish the requirement of industrial conditions and easily lose their activity in harsh environments. In this study, a novel α-amylase named PersiAmy1 has been identified through the multistage in silico screening pipeline from the rumen metagenomic data. The long-term storage of PersiAmy1 in low and high temperatures demonstrated 82.13 and 71.01% activities after 36 days of incubation at 4 and 50°C, respectively. The stable α-amylase retained 61.09% of its activity after 180 min of incubation at 90°C and was highly stable in a broad pH range, showing 60.48 and 86.05% activities at pH 4.0 and pH 9.0 after 180 min of incubation, respectively. Also, the enzyme could resist the high-salinity condition and demonstrated 88.81% activity in the presence of 5 M NaCl. PersiAmy1 showed more than 74% activity in the presence of various metal ions. The addition of the detergents, surfactants, and organic solvents did not affect the α-amylase activity considerably. Substrate spectrum analysis showed that PersiAmy1 could act on a wide array of substrates. PersiAmy1 showed high stability in inhibitors and superb activity in downstream conditions, thus useful in detergent and baking industries. Investigating the applicability in detergent formulation, PersiAmy1 showed more than 69% activity after incubation with commercial detergents at different temperatures (30–50°C) and retained more than 56% activity after incubation with commercial detergents for 3 h at 10°C. Furthermore, the results of the wash performance analysis exhibited a good stain removal at 10°C. The power of PersiAmy1 in the bread industry revealed soft, chewable crumbs with improved volume and porosity compared with control. This study highlights the intense power of robust novel PersiAmy1 as a functional bio-additive in many industrial applications.

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α-Amylase production by Tepidimonas fonticaldi strain HB23: statistical optimization and compatibility study for use in detergent formulations

Cited by 5 publications

References 18 publications

Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

Highly Efficient Computationally Derived Novel Metagenome α-Amylase With Robust Stability Under Extreme Denaturing Conditions

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