α-Amylase Inhibitors from Cowpea Seeds

Melo, Francislete Rodrigues; Sales, Maurı́cio P.; Pereira, Lucilene S.; Bloch, Carlos; Franco, Octávio Luiz; Ary, Maria B.

doi:10.2174/092986650606221117144709

Cited by 32 publications

(3 citation statements)

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“…Le Berre-Anton et al [41] identified an α-AI extracted from the seeds of Phaseolus vulgaris L. called αAl-1. Furthermore, a case study of enzyme inhibitors of the seed extract from Vigna unguiculata L. revealed up to a 50% decrease in amylolytic activity in larvae of Callosobruchus maculatus F [30]. The application of bean proteinaceous…”

Section: Discussionmentioning

confidence: 99%

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Potential Biopesticides from Seed Extracts: A Sustainable Way to Protect Cotton Crops from Bollworm Damage

Chamani,

Askari,

Pourabad

et al. 2023

Sustainability

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In the current study, the inhibitory effect of extracts from Gramineae (wheat, barley, and corn) and Leguminosae (sophora, bean, and pea) seeds was studied on the digestive alpha-amylase activity in cotton bollworm (Helicoverpa armigera Hubner). The insect was bred on artificial food based on Vigna unguiculata in the greenhouse condition (26 ± 2 °C, 60 ± 10% (Relative Humidity), R.H., 16:8 (Light: Darkness), L: D). The extracts of wheat (95.2%), barley (84.6%), corn (73.8%), sophora (77%), Vigna unguiculata (52%), and pea (56.7%) significantly inhibited the alpha-amylase activity in H. armigera. Studying the impact of different fractions (obtained via deposition at various concentrations of ammonium sulfate salt) on the alpha-amylase enzyme activity demonstrated that in 0–30% fractions, wheat, barley, and sophora have the highest effect (95.26%, 94.65%, and 94.73%, respectively) compared to the other fractions. The inhibitory activities of 0–30% fractions of corn, bean, and pea were 83.3, 56.94, and 50.92%, respectively. In 30–50% fractions, the most effective ones were those of wheat and barley with the inhibitory activity of 79.7% and 82.9%, respectively. In addition, bean and pea fractions inhibited 25.2% and 27.5%, in that order. No significant inhibitory impact was detected in 50–70% or higher fractions. The investigation of the impact of pH values (i.e., 2, 4, 8, and 10) on the inhibition of the alpha-amylase enzyme activity introduced 8–10 as the optimum pH in H. armigera. Nanotechnology offers several ways to enhance plant-based pesticides, which are a solution for making plant extract usage more efficient. The exploration of plant-based pesticides, in conjunction with the incorporation of nanotechnology and other scientific fields, offers a wide range of prospects for further investigation.

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Section: Discussionmentioning

confidence: 99%

“…The obtained mixture was mixed slowly on a magnetic stirrer for 90 min at room temperature, then centrifuged (10,000 rpm, 4 • C, 30 min). The resulting liquid was transferred into microtubes and kept in the freezer (80 • C) as the inhibitor extract to conduct studies [29,30].…”

Section: Preparation Of Various Plant Extracts and Fractionsmentioning

confidence: 99%

Potential Biopesticides from Seed Extracts: A Sustainable Way to Protect Cotton Crops from Bollworm Damage

Chamani,

Askari,

Pourabad

et al. 2023

Sustainability

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“…Commercial medications for α-glucosidase inhibition in the treatment of diabetic illness include acarbose, miglitol, and voglibose [ 5 ]. Chronic inhibition of α-amylase and α-glucosidase may be beneficial in the treatment of type 2 diabetes and obesity [ 6 , 7 ]. As reversible inhibitors of α-glucosidase and α-amylase, the medications that are currently used to treat diabetic patients have adverse effects such as gastrointestinal disorders, meteorism, gas, bloating, and possibly even diarrhea [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring Edible Mushrooms for Diabetes: Unveiling Their Role in Prevention and Treatment

et al. 2023

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Diabetes mellitus is a complex illness in which the body does not create enough insulin to control blood glucose levels. Worldwide, this disease is life-threatening and requires low-cost, side-effect-free medicine. Due to adverse effects, many synthetic hypoglycemic medications for diabetes fail. Mushrooms are known to contain natural bioactive components that may be anti-diabetic; thus, scientists are now targeting them. Mushroom extracts, which improve immune function and fight cancer, are becoming more popular. Mushroom-derived functional foods and dietary supplements can delay the onset of potentially fatal diseases and help treat pre-existing conditions, which leads to the successful prevention and treatment of type 2 diabetes, which is restricted to the breakdown of complex polysaccharides by pancreatic-amylase and the suppression of intestinal-glucosidase. Many mushroom species are particularly helpful in lowering blood glucose levels and alleviating diabetes symptoms. Hypoglycaemic effects have been observed in investigations on Agaricussu brufescens, Agaricus bisporus, Cordyceps sinensis, Inonotus obliqus, Coprinus comatus, Ganoderma lucidum, Phellinus linteus, Pleurotus spp., Poria cocos, and Sparassis crispa. For diabetics, edible mushrooms are high in protein, vitamins, and minerals and low in fat and cholesterol. The study found that bioactive metabolites isolated from mushrooms, such as polysaccharides, proteins, dietary fibers, and many pharmacologically active compounds, as well as solvent extracts of mushrooms with unknown metabolites, have anti-diabetic potential in vivo and in vitro, though few are in clinical trials.

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