α-amylase, glycogen synthetase and phosphorylase in the human endometrium: Influence of the cycle and of the Cu-T device

Robles, F.; Osa, E.López de la; Lerner, U; Johannisson, E.; Brenner, Paul H.; Hagenfeldt, Kerstin; Diczfalusy, E.

doi:10.1016/0010-7824(72)90038-8

Cited by 21 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mid-secretory endometrium also has prominent peaks in the lipid region (1735 cm −1 ), Amide I (1666, 1627, 1597 cm −1 ), Amide II (1516 cm −1 ), other protein regions (1481, 1435 cm −1 ), ν as PO 2 − (1215 cm −1 ), and symmetric phosphate stretching vibrations ( ν s PO 2 − (1095 cm −1 )) regions. The largest peak is at 1026 cm −1 ; this corresponds with glycogen, a biochemical substrate known to be present at high levels in mid-secretory endometrium (Robles et al , 1972). …”

Section: Resultsmentioning

confidence: 99%

Infrared spectroscopy with multivariate analysis to interrogate endometrial tissue: a novel and objective diagnostic approach

et al. 2011

View full text Add to dashboard Cite

Background:Endometrial cancer is the most common gynaecological malignancy in the United Kingdom. Diagnosis currently involves subjective expert interpretation of highly processed tissue, primarily using microscopy. Previous work has shown that infrared (IR) spectroscopy can be used to distinguish between benign and malignant cells in a variety of tissue types.Methods:Tissue was obtained from 76 patients undergoing hysterectomy, 36 had endometrial cancer. Slivers of endometrial tissue (tumour and tumour-adjacent tissue if present) were dissected and placed in fixative solution. Before analysis, tissues were thinly sliced, washed, mounted on low-E slides and desiccated; 10 IR spectra were obtained per slice by attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy. Derived data was subjected to principal component analysis followed by linear discriminant analysis. Post-spectroscopy analyses, tissue sections were haematoxylin and eosin-stained to provide histological verification.Results:Using this approach, it is possible to distinguish benign from malignant endometrial tissue, and various subtypes of both. Cluster vector plots of benign (verified post-spectroscopy to be free of identifiable pathology) vs malignant tissue indicate the importance of the lipid and secondary protein structure (Amide I and Amide II) regions of the spectrum.Conclusion:These findings point towards the possibility of a simple objective test for endometrial cancer using ATR-FTIR spectroscopy. This would facilitate earlier diagnosis and so reduce the morbidity and mortality associated with this disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Infrared spectroscopy with multivariate analysis to interrogate endometrial tissue: a novel and objective diagnostic approach

et al. 2011

View full text Add to dashboard Cite

show abstract

Immunohistochemical demonstration of pancreatic α-amylase and trypsin in intrahepatic bile ducts and peribiliary glands

Terada

Nakanuma

1991

Hepatology

View full text Add to dashboard Cite

Epithelia of intrahepatic bile ducts and peribiliary glands were immunohistochemically examined for pancreatic alpha-amylase and trypsin in 54 normal autopsied livers. alpha-Amylase was evaluated with a polyclonal antibody, and trypsin was assayed with both polyclonal and monoclonal antibodies. alpha-Amylase was observed in large ducts, septal ducts and peribiliary glands in most livers and was seen in interlobular ducts in seven (13%) livers. Trypsin immunoreactivity with the polyclonal antibody was observed in peribiliary glands in 21 (39%) livers; it was absent in intrahepatic bile ducts in all but one liver. Trypsin immunoreactivity with the monoclonal antibody was present in large ducts, septal ducts and peribiliary glands in about 70% of the livers and was seen in interlobular ducts in two (4%) livers. Bile ductules were always negative for the two antigens. Some epithelia of peribiliary glands positive for both alpha-amylase and trypsin histologically resembled pancreatic acinar cells. alpha-Amylase and trypsin immunoreactivities of intrahepatic biliary epithelia and pancreatic aninar cells were eliminated by absorption of primary antibodies by alpha-amylase or trypsin, suggesting the specificities of the immunoreactivities. These data suggest that epithelia of intrahepatic large ducts, septal ducts and peribiliary glands contain pancreatic alpha-amylase in most livers and that they contain trypsin in about 70% of livers. alpha-Amylase and trypsin may be secreted into intrahepatic bile duct lumens, thereby exerting important effects on the physiology of the intrahepatic biliary tree and hepatic bile.

show abstract

The Human α-Amylases

Merritt

Karn

1977

Advances in Human Genetics 8

View full text Add to dashboard Cite

α-amylase, glycogen synthetase and phosphorylase in the human endometrium: Influence of the cycle and of the Cu-T device

Cited by 21 publications

References 12 publications

Infrared spectroscopy with multivariate analysis to interrogate endometrial tissue: a novel and objective diagnostic approach

Infrared spectroscopy with multivariate analysis to interrogate endometrial tissue: a novel and objective diagnostic approach

Immunohistochemical demonstration of pancreatic α-amylase and trypsin in intrahepatic bile ducts and peribiliary glands

The Human α-Amylases

Contact Info

Product

Resources

About