α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway

Zhang, Siwen; Wang, Junfu; Chen, Ting; Wang, Jiancheng; Wang, Ye; Zhu, Yu; Zhao, Kun; Zheng, Kaitian; Chen, Yeyang; Wang, Zhen; Li, Bopei; Wang, Congjun; Huang, Weining; Zhao, Fen; Chen, Junqiang

doi:10.1080/21655979.2021.1967713

Cited by 21 publications

(13 citation statements)

References 62 publications

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“…MYOF is expressed in both cardiac and skeletal muscle and plays a crucial role in muscle development by regulating the canonical Wnt signaling pathway 50 , 51 . Meanwhile, ACTN1 and ACTN4 are involved in actin filament bundling, cell adhesion and cell migration in non-muscle cells 52 , 53 . Apart from the AKV’s gene families, prominent NWV-specific gene families have been linked to the sense of smell, including more than 10 olfactory receptor gene families.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive genome assembly reveals genetic diversity and carcass consumption insights in critically endangered Asian king vultures

Buthasane,

Shotelersuk,

Chetruengchai

et al. 2024

Sci Rep

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The Asian king vulture (AKV), a vital forest scavenger, is facing globally critical endangerment. This study aimed to construct a reference genome to unveil the mechanisms underlying its scavenger abilities and to assess the genetic relatedness of the captive population in Thailand. A reference genome of a female AKV was assembled from sequencing reads obtained from both PacBio long-read and MGI short-read sequencing platforms. Comparative genomics with New World vultures (NWVs) and other birds in the Family Accipitridae revealed unique gene families in AKV associated with retroviral genome integration and feather keratin, contrasting with NWVs’ genes related to olfactory reception. Expanded gene families in AKV were linked to inflammatory response, iron regulation and spermatogenesis. Positively selected genes included those associated with anti-apoptosis, immune response and muscle cell development, shedding light on adaptations for carcass consumption and high-altitude soaring. Using restriction site-associated DNA sequencing (RADseq)-based genome-wide single nucleotide polymorphisms (SNPs), genetic relatedness and inbreeding status of five captive AKVs were determined, revealing high genomic inbreeding in two females. In conclusion, the AKV reference genome was established, providing insights into its unique characteristics. Additionally, the potential of RADseq-based genome-wide SNPs for selecting AKV breeders was demonstrated.

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Section: Discussionmentioning

confidence: 99%

Comprehensive genome assembly reveals genetic diversity and carcass consumption insights in critically endangered Asian king vultures

Buthasane,

Shotelersuk,

Chetruengchai

et al. 2024

Sci Rep

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“…13 Elevated ACTN1 expression has been documented in a spectrum of cancers, including but not limited to gastric cancer, hepatocellular carcinoma, and oral squamous cell carcinoma. [14][15][16] For instance, Zhang et al demonstrated that ACTN1 over-expression in gastric cancer disrupts the cell cycle and attenuates apoptosis by modulating the AKT/GSK3b/b-catenin signaling axis. 14 In hepatocellular carcinoma, ACTN1 up-regulation has been linked to the suppression of the Hippo signaling pathway through its interaction with MOB1.…”

Section: Discussionmentioning

confidence: 99%

ACTN1 promotes malignant progression in Cutaneous Squamous Cell Carcinoma by reducing the p53 signaling pathway

Yuan,

Lu,

Jiang

et al. 2023

Preprint

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Background: Cutaneous Squamous Cell Carcinoma (CSCC) presents a complex pathological landscape. While extensive research has been conducted to elucidate the molecular underpinnings of CSCC tumorigenesis, the role of ACTN1 remains ambiguous. ACTN1 has been implicated in various malignancies, but its relationship with CSCC is not yet clearly defined. Objectives: This study aims to investigate the functional role and underlying molecular mechanisms of ACTN1 in the progression of CSCC. Methods: A total of 10 normal skin tissues, 30 Bowen's disease tissues and 80 CSCC tissues between January 2019 and June 2021 in the Department of Pathology and Dermatology in our hospital were enrolled. The ACTN1 expression in 120 tissues samples were conducted by immunohistochemistry staining methods. The 3 patients with CSCC were enrolled to further verified the ACTN1 protein expression level difference between CSCC tissues and paracancerous tissues. The functions and potential molecular mechanisms of ACTN1 in CSCC is still unclear. Firstly, the ACTN1 protein and mRNA expression between CSCC cell lines (Colon-16, SCL-1 and A431) and a control cell line (hacat cell) were investigated. Besides, the ability of proliferation, migration and invasion after siRNA-mediated ACTN1 knockdown in SCL-1 and A431 cell lines were performed via cell counting kit-8 (CCK-8), wound healing and transwell assays, respectively. The RNA-Seq analysis of the si-con and si-ACTN1 in SCL-1 cells was performed to further investigate the molecular mechanisms underlying ACTN1-mediated malignant biological behavior in CSCC. Finally, the apoptosis-promoting and apoptosis-inhibiting proteins, cell cycle proteins and p53 protein and mRNA levels were investigated. Results: ACTN1 expression levels was significantly elevated in CSCC compared to Bowen's disease (P<0.001). The protein was predominantly localized in the cytoplasm, manifesting as brownish-yellow and tan granules. Elevated ACTN1 levels was correlated with worsen cell differentiation and increased depth of skin invasion (P<0.05). The ACTN1 protein expression levels in CSCC tissues were significantly higher than that in paired paracancerous tissues. Besides, when compared with the control group (hacat cell), higher expression of ACTN1 protein and mRNA in A431, SCL-1 and Colon-16 line cells were also verified. After interfering with the expression of ACTN1, the ability of proliferation, migration and invasion ability in the SCL-1 and A431 cells were significantly decreased (P<0.05). A total of 294 gens were differential expressed between the si-con and si-ACTN1 in SCL-1 cells line, with 165 up-regulated and 129 down-regulated. The KEGG enrichment analysis demonstrated that the top five significantly enriched related signaling pathways were complement and coagulation cascades, p53 signaling pathway, IL-17 signaling pathway, PPAR signaling pathway and staphylococcus aureus infection. Furthermore, all differential expressed genes were enrolled to explore the hub genes according to degree algorithm via Cytoscape software. Two clusters were formed and theses two clusters mainly involved in the p53 signaling pathways. The qRT-PCR and Western blot results showed that after si-ACTN1, the mRNA and protein expression levels of p53 and pro-apoptotic factor Bax were significantly up-regulated, while the apoptosis inhibitor BCL2 and Cyclin D were down-regulated. After constructed the siRNA targeting p53 and ACTN1 in A431 and SCL-1 cell lines, the qRT-PCR and Western blot results showed that the mRNA and protein expression levels of p53, Bax, BCL2 and Cyclin D were not significantly changed. Conclusions: ACTN1 serves as a pivotal regulator in CSCC progression, primarily through its modulation of the p53 signaling pathway. Our findings offer new avenues for targeted therapeutic strategies in CSCC management.

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“…Previous study indicated that the ACTN1 regulates the epithelial-mesenchymal transition and tumorigenesis of gastric cancer via the AKT/GSK3 β/β-catenin pathway. 9 Besides, the ACTN1 is also over-expressed in hepatocellular carcinoma tissues by suppressing Hippo signaling via physical interaction with MOB1. 10 On the other hand, higher ACTN1 protein levels were significantly associated with poor prognosis in Oral Squamous Cell Carcinoma.…”

Section: Cutaneous Squamous Cell Carcinoma (Cscc) (Nmentioning

confidence: 99%

The value of ACTN1 in the diagnosis of cutaneous squamous cell carcinoma: A continuation study

Yuan

Xiao‐hong

et al. 2023

Skin Research and Technology

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α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway

Cited by 21 publications

References 62 publications

Comprehensive genome assembly reveals genetic diversity and carcass consumption insights in critically endangered Asian king vultures

Comprehensive genome assembly reveals genetic diversity and carcass consumption insights in critically endangered Asian king vultures

ACTN1 promotes malignant progression in Cutaneous Squamous Cell Carcinoma by reducing the p53 signaling pathway

The value of ACTN1 in the diagnosis of cutaneous squamous cell carcinoma: A continuation study

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