α-4-O-methyl-d-glucuronidase component of xylanolytic complexes

A Chromogenic Substrate for a β-Xylosidase-Coupled Assay of α-Glucuronidase

“…There have been several successful attempts to synthesize 4-nitrophenyl ␣-D-glucuronide as a potential chromogenic substrate of ␣-glucuronidase (7,25,26). However, none of the tested microbial ␣-glucuronidases was found to be capable of hydrolyzing this substrate.…”

“…A nonmodified procedure for determining reducing sugars was used in the case of aldouronic acids reduced with NaBH 4 (9). Alternative methods include HPLC determination of free MeGlcA (19,20) or the equivalent amount of xylooligosaccharide (12,21), or GLC of its trimethylsilyl oxim derivative (7,(22)(23)(24).…”

Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

“…The products of the xylan hydrolysis were analyzed by thin-layer chromatography on silica gel (Silica Gel 60 AL TLC Merck), using solvent system composed of ethyl acetate, acetic acid, formic acid and water (9:3:1:4 v/v), according to Fontana et al, 1988. The hydrolysis products were detected with 0.2% of orcinol (w/v) in sulfuric acid and methanol (10:90 v/v).…”

Partial characterization of a novel amylase activity isolated from TunisianFicus caricalatex

“…The spots were visualized by spraying TLC plates with H 2 SO 4 /methanol (5:95, v/v) followed by heating at 120°C for 10 min (Fontana et al, 1988).…”