α-2-L-Fucosyltransferase Activity in Sera of Individuals with H-Deficient Red Cells and Normal H Antigen in Secretions

Abstract

α-2-L-Fucosyltransferase activity was found in the sera of 4 H-deficient secretor individuals (H(z)). This activity represented about 5-10% of the activity present in the serum of normal H phenotypes.

Detection of Combined ABH and Lewis Glycosphingolipids in Sera of H‐Deficient Donors

“…The OfLeb donor completely lacked the H and A antigens on red cells and the H enzyme in serum, but was known to be ofA/-genotype because of a normal A glycosyltransferase in serum [31]. The 0, Leb serum was obtained from a genotypically 0/0 donor who was H-deficient on red cells and H-deficient in serum, as measured by his a-2-L-fucosyltransferase activity [32], but who had normal H antigen in saliva. The A,Leb and the 0 Leh donors served as the corresponding H-normal, Lewis-positive, salivary secretor controls (red cell phenotype Le(a-b+) and salivary phenotype Leh).…”

A rapid molecular method (polymerase chain reaction with sequence‐specific primers) to genotype for ABO blood group and secretor status and its potential for organ transplants

“…Expression of A and B antigens in the kidney is regulated by the H (FUTI) and Se (FUTII) genes, as well as by A and B transferase genes. The H and Se genes code for distinct a( 1,2)fucosyltransferases (16)(17)(18); these both catalyze the synthesis of the H antigen but have distinct tissue-specificity and differential preference for certain precursor type chains (5,19,20). From the literature we can predict that a non-secretor (homozygous for the null se gene) would have significantly reduced expression of H antigen in certain tissues, including the distal convoluted and collecting tubules of the kidney and thus reduced expression of A and/or B antigens.…”

ABO, H, and Lewis Systems

“…O h -secretor red cells are not usually agglutinated by anti -A or -B, but some O h A -secretor cells behave like A x cells and are agglutinated by anti -A,B and very potent anti -A[439,442] . A similar variation exists with B antigen strength in O h B -secretors[80,440,442,443] .Like those of most secretors, O h -secretor red cells are usually Le(a -b + ), but may be Le(a -b -). A similar variation exists with B antigen strength in O h B -secretors[80,440,442,443] .Like those of most secretors, O h -secretor red cells are usually Le(a -b + ), but may be Le(a -b -).…”

“…Most H -defi cient secretors have been found in Eastern Asia -Chinese [408,412,441,446] and Japanese [406,409,440] -but also very rarely in other ethnic groups including people of European origin [80,411,443,447] , from the Middle East [80,411] , and a Native American [448] . Some estimated frequencies: one in 5000 Thais [442] , one in 8000 Taiwanese [449] , and one in 15 620 Hong Kong Chinese [441] .…”

“…A secretor of B and H with H -defi cient red cells was subsequently found [440] . [80,440] . [80,440] .…”