Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from 'Kombucha tea'

Kurtzman, C. P.

doi:10.1016/s1567-1356(01)00021-6

Cited by 23 publications

(30 citation statements)

References 8 publications

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“…The second group includes other species currently assigned to Zygosaccharomyces that are not part of the Z. rouxii clade, on the basis of rDNA analysis, viz. Z. cidri, Z. fermentati, Z. florentinus, Z. microellipsoides and Z. mrakii (Kurtzman et al, 2001). Based on multi-gene sequence analysis, Kurtzman recently reviewed the genus, splitting the 11 Zygosaccharomyces species into three phylogenetically circumscribed genera, viz.…”

Section: Solieri S Cassanelli and P Giudicimentioning

confidence: 99%

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A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

Solieri

Cassanelli

Giudici

2007

Yeast

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The taxonomic status and species number of the genus Zygosaccharomyces have rapidly changed in the last years. In this study, two new osmotolerant Zygosaccharomyces strains isolated from traditional balsamic vinegar, viz. ABT301 and ABT601, were investigated to elucidate their taxonomic relationships with Zygosaccharomyces rouxii species. A multi-gene sequence approach was employed, including regions of the rDNA repeat [5.8S, two internal transcribed spacers (ITS) and the 26S D1/D2 domain], COX2 mitochondrial gene and two nuclear genes (SOD2 and HIS3 ). Cloning and sequence analysis of 5.8S-ITS rDNA revealed that these strains bear an unusual polymorphism for this region. Three highly divergent 5.8S-ITS sequences were detected, one identical to Z. rouxii, the other two showing some relatedness to Z. mellis. Sequence and gene number polymorphism was also observed for the protein-encoding nuclear genes SOD2 and HIS3, as two copies for each gene different from those found in Z. rouxii were detected. Analysis of the D1/D2 26S domain showed that ABT301 and ABT601 have only one type of D1/D2 sequence statistically different from that of Z. rouxii. The findings obtained in this work suggest that the genomic background of strains ABT301 and ABT601 is different from the other Zygosaccharomyces species. We speculated that they could belong to a new putative species related to Z. rouxii.

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Section: Solieri S Cassanelli and P Giudicimentioning

confidence: 99%

“…Nine species are currently accepted in the genus (Kurtzman, 1998) and recently two new species, Z. lentus (Steels et al, 1999) and Z. kombuchaensis, have been proposed (Kurtzman et al, 2001;Steels et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

Solieri

Cassanelli

Giudici

2007

Yeast

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“…Accordingly, the genus Kodamaea Yamada, Suzuki, Matsuda et Mikata was proposed for that species (Yamada et al 1995b). In this study, Kodamaea ohmeri also found in A. florea, showed a strong (Kurtzman and Robnett 1998;Kurtzman et al 2001;Rosa and Lachance 2005;Steels et al 1999). Bootstrap values were calculated from 1,000 replicates, and values below 50% were omitted.…”

Section: Resultsmentioning

confidence: 67%

A novel ascosporogenous yeast species, Zygosaccharomyces siamensis, and the sugar tolerant yeasts associated with raw honey collected in Thailand

et al. 2011

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Diversity of yeasts in association with bees and their food sources has been explored during the last decade. In Thailand, there has been no study of yeast identification in honey and bees. Hence, a total of 186 yeast strains were isolated from 37 honey samples of 12 different bee species. On the basis of morphological and physiological characteristics, 55 representative strains were chosen and identified by sequence analysis of the 26S rDNA D1/D2 domain and the ITS region. The data were compared with the published sequences and the results showed the occurrence of 19 ascomycetous and 1 basidiomycetous yeast species. Six strains of the new species were isolated. Phylogenetic analysis of the 26S rDNA D1/D2 sequence revealed that they were conspecific and most closely related to Zygosaccharomyces mellis. Based on the ITS sequence, the new species was clustered with the type " and clearly distinguished from the type !. Sequence analysis of combined ITS-26S rDNA D1/D2 showed similar results. The occurrence of these two types, with a divergence of more than 1% in their sequences, and low DNA relatedness among them suggested that members of the type β can be regarded as separate species. An analysis of the morphological and physiological characteristics was performed. Ascospore formation was observed on acetate agar and Gorodkowa agar. The new Zygosaccharomyces species differed physiologically from Z. mellis in 4 assimilation tests. This data supports the hypothesis that the new species, Zygosaccharomyces siamensis, is a novel ascosporogenous yeast. The type strain is JCM 16825 T (=CBS 12273 T ) and a description is given here.

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“…Identification of yeast other than Candida was performed by ethanol tolerance test (Guimarães et al, 2006). Acetic acid tolerance test was also performed using acidified media (Kurtzman et al, 2001). …”

Section: Biochemical Characterization Of Yeastmentioning

confidence: 99%

Isolation and Characterization of Bacteria and Yeast from Kombucha Tea

Mukadam¹,

Punjabi²,

Deshpande³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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Zygosaccharomyces kombuchaensis, a new ascosporogenous yeast from 'Kombucha tea'

Cited by 23 publications

References 8 publications

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

A novel ascosporogenous yeast species, Zygosaccharomyces siamensis, and the sugar tolerant yeasts associated with raw honey collected in Thailand

Isolation and Characterization of Bacteria and Yeast from Kombucha Tea

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