Zwitterionic polymer-coated immunobeads for blood-based cancer diagnostics

Kim, Hyung-Seok; Yong, Yeryoung; Kang, Hyun Ju; Park, Kyunghee; Kim, Kyung Sik; Lee, Myoyong; Huh, Nam

doi:10.1016/j.biomaterials.2013.09.101

Cited by 16 publications

(16 citation statements)

References 28 publications

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“…A study by Kim et al [85] prepared modified zwitterionic sulfobetaine-conjugated immunobeads using CD83 as a candidate protein marker for breast cancer derived microvesicles. The zwitterionic immunobeads are very efficient at isolating tumor derived-mirovesicles TMVs from clinical plasma samples by suppressing nonspecific protein binding than conventional immunobeads.…”

Section: Circulating Micrornas Reviewmentioning

confidence: 99%

Circulating microRNAs: potential biomarkers for common malignancies

Khoury

Tran

2015

Biomark. Med.

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MicroRNAs (miRNAs) belong to a class of small noncoding RNAs (ncRNAs), which regulate gene expression at the post-transcriptional level. They are approximately 22 nucleotide sequences in length and have been predicted to control expression of up to 30-60% of all protein-coding genes in mammals. Considering this wide involvement in gene control, aberrant miRNA expression has a strong association with the presence and progression of a disease, hence generating much anticipation in using miRNAs as biomarkers for the diagnosis and prognosis of human cancers. The majority of these miRNAs are intracellular, but recently they have been discovered in bodily fluids. This review will provide an insight into these circulatory miRNA molecules and discuss their potential as cancer biomarkers.

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Section: Circulating Micrornas Reviewmentioning

confidence: 99%

Circulating microRNAs: potential biomarkers for common malignancies

Khoury

Tran

2015

Biomark. Med.

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“…Tumor derived microvesicles have been proposed as biomarkers for blood based cancers (Kim, Yong et al 2014). Zwitterionic sulfobetaine-conjugated immunobeads are readied utilizing cluster of separation 83 (CD83) as a hopeful protein marker for breast tumor derived microvesicles.…”

Section: Other Methodsmentioning

confidence: 99%

How far we have come in detecting Circulating Tumor Cells?

Ghosh¹

2014

IOSRJPBS

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Fluid biopsies dependent upon recognition and profiling of circulating tumor cells (CTCs) offer a non-obtrusive instrument, encouraging unanticipated disease analysis and treatment monitoring. CTCs attack the remote organs by means of the circulatory framework. It has been assessed that primary tumor tosses a million cells day by day into dissemination. Notwithstanding, the majority of these cells bite the dust in the circulatory framework and just the fittest achieve the ordained area to cause secondary tumors. The identification of CTCs in the blood and their characterization can furnish knowledge into the metastatic potential of the primary tumor and the phase of the metastasis. This review aims to provide the reader a glimpse of CTCs, their importance in tumor recognition, different systems for distinguishing and separation of CTCs from blood, their characterization and the challenges ahead to make CTCs a dependable and effective diagnostic device. Such advancement will possibly exchange traditional biopsy presenting new period of customised cancer management.

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“…58 As a result, materials derived from SB or CB are considered as biomimetic. 1 Their biomimetic nature along with their excellent antifouling characteristics make them interesting for a wide range of applications, 13,59 such as biosensing, [60][61] drug delivery, 12,62 filtration, 51,[63][64] as well as for antibacterial 65 and marine coatings. 15,66 A fundamental difference between SB and CB is that CB-based materials can be directly functionalized (mostly via EDC/NHS chemistry to attach antibodies, see below), and are therefore often used for biosensing applications.…”

Section: Phosphocholinementioning

confidence: 99%

“…77,90 As a result, SI-ATRP has been established as an excellent technique to grow polymer brushes, which can be used in the biomedical field with applications in separation, antibacterial and/or antifouling materials, controlled cell adhesion, and biosensing. 10,15,59 SI-ATRP has been used throughout the research chapters of this thesis.…”

Section: Surface-initiated Atom Transfer Radical Polymerization (Si-amentioning

confidence: 99%

“…There are various studies that show improved performances of the microbeads by coating them with PEG, [57][58] and several examples that incorporate zwitterionic moieties have also been reported. [59][60] However, coatings using zwitterionic polymer brushes installed via the grafting-from approach are scarce. The beads developed in chapter 3 can be readily applied in existing protocols due to their compatibility for flow cytometry measurements.…”

Section: Applications Of Antifouling Beadsmentioning

confidence: 99%

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Romantic surfaces : Zwitterionic polymer brushes for biomedical applications

Andel¹

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In order to enhance the sensitivity and selectivity of surface-based (bio)sensors, it is of crucial importance to diminish background signals that arise from the non-specific binding of biomolecules, so-called biofouling. Zwitterionic polymer brushes have been shown to be excellent antifouling materials. However, for sensing purposes, antifouling does no suffice, but needs to be combined with the possibility to efficiently modify the brush with recognition units. So far this has only been achieved at the expense of either antifouling properties or binding capacity. Herein we present a conceptually new approach by integrating both characteristics into a single, tailor-made monomer: a novel sulfobetainebased zwitterionic monomer equipped with a clickable azide moiety. Copolymerization of this monomer with a well-established standard sulfobetaine monomer results in highly antifouling surface coatings with a high, yet tunable amount of clickable groups present throughout the entire brush. Subsequent functionalization of the azido-brushes, via e.g. widely used strain-promoted alkyne azide click reactions yields fully zwitterionic 3D-functionalized coatings with a recognition unit of choice that can be tailored for any specific application. Here we show a proof of principle with biotin-functionalized brushes on Si 3 N 4 that combine excellent antifouling properties with specific avidin binding from a protein mixture. The signal-to-noise ratio is significantly improved over traditional chain end modification of sulfobetaine polymer brushes, even if the azide content is lowered to 1%. This therefore offers a viable approach for the development of biosensors with greatly enhanced performance. Non-specific adsorption of biomolecules to solid surfaces, a process called biofouling, is a major concern in many biomedical applications. Great effort has been made in the development of antifouling polymer coatings that are capable of repelling the non-specific adsorption of proteins, cells and microorganisms. In this respect, we herein contribute to the understanding of which polymer brush results in the best antifouling coating. To this end, we compared five different monomers: two sulfobetaines, a carboxybetaine, a phosphocholine and a hydroxyl acrylamide. The antifouling coatings were analyzed using the in chapter 3 described bead-based method with flow cytometry as read-out system. This method allows for the quick and automated analysis of thousands of beads per second, enabling fast analysis and good statistics. We report the first direct comparison made between a sulfobetaine with opposite charges separated by two and three methylene groups and a carboxybetaine bearing two separating methylene groups. It was concluded that both the distance between opposite charges and the nature of the anionic groups have a distinct effect on the antifouling performance. Phosphocholines and simple hydroxyl acrylamides are not often compared with the betaines. However, here we found that they perform equally well or even better, with as ove...

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Zwitterionic polymer-coated immunobeads for blood-based cancer diagnostics

Cited by 16 publications

References 28 publications

Circulating microRNAs: potential biomarkers for common malignancies

Circulating microRNAs: potential biomarkers for common malignancies

How far we have come in detecting Circulating Tumor Cells?

Romantic surfaces : Zwitterionic polymer brushes for biomedical applications

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