Zwitterionic molecularly imprinted polymer‐based solid‐phase micro‐extraction coupled with molecularly imprinted polymer sensor for ultra‐trace sensing of L‐histidine

Abstract

The proposed L-histidine sensing system composed of a molecularly imprinted solid-phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non-specific sorption and false-positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid-phase microextraction material. The hyphenated approach was able to detect L-histidine quantitatively with a limit of detection as low as 0.0435 ng/mL (RSD = 0.2%, S/N = 3).

Advancement in Analytical and Bioanalytical Techniques as a Boon to Medical Sciences

“…Although routine clinical methods indicate normal/ abnormal levels of bioanalytes in urine/blood, still they often lack specificity due to severe complex biological sample interferences. In this regard, sample cleanup and highly sensitive techniques have proven to be helpful for early-stage disease diagnosis [52,53] and detecting medical abnormalities [54]. To date, an array of hyphenated techniques plays an important role in the determination of bioanalytes, with improved selectivity and sensitivity.…”

Flexible Electrochemical Sensors and Their Applications in Noninvasive Medical Detection★

“…电化学生物传感器是一类基于生物亲和识别的新 型传感器, 其结构通常包含用于特异性识别目标分子的 生物分子敏感元件, 以及将识别事件转化为电学信号的 信号转换元件, 通过关联电学信号与目标分子浓度来实 现检测 [8] . 生物敏感元件的种类多样, 包括抗体 [107][108] 、 核酸适配体 [109][110] 、分子印迹聚合物(molecularly imprinted polymer, MIP) [111][112] 和细菌 [113] 等. 与前述的目标 识别机制(离子选择性识别、酶促反应和直接氧化等)相 比, 基于生物敏感元件的传感器具有高度的特异性和灵 敏性, 适用于定量检测生物流体中与疾病相关的超低浓 度(nmol•L −1 或更低)生物标志物, 如激素和蛋白质等.…”

分子印迹样品前处理技术的研究进展

“…固 相 微 萃 取 (Solid phase microextraction, SPME) [51,52] [54,56,57] 、血浆 [55] 、脑 脊髓液 [56] 、 医药品 [54,56] 等复杂样品中目标分子的分离 富集, 但是没能实现在线检测. Li等 [58] 制备了多溴联 苯醚BDE-209 为模板分子的有机修饰硅纤维固相微 萃取涂层, 对BDE-209 的富集能力远优于商用聚丙 烯酸酯和聚二甲基硅氧烷涂层.…”