Zwint is highly expressed in ovarian cancer and promotes cell proliferation, migration and invasion  

Abstract

Objective: To investigate the expression level of ZWINT in ovarian cancer and its impact on the proliferation, migration and invasion of ovarian cancer cells. Methods: The differentially expressed genes in ovarian cancer tissues were identified from both the TCGA and GEO databases, followed by an analysis of their expression levels and investigation into their impact on patients' survival and prognosis; two ovarian cell models were established to interfere with gene expression, and the expression and interference effects were evaluated by RT-PCR and Western blot; cell proliferation was detected by plate cloning and Edu assay. Result: ZWINT was identified as a differentially expressed gene in ovarian cancer through database analysis, and further comparison of gene expression profiles revealed its high expression levels in this malignancy; Kaplan-Meier analysis revealed a correlation between higher expression of ZWINT and worse survival prognosis in patients. The success of interfering with the ZWINT cell line was verified through RT-PCR and Western blot experiments. Plate cloning assay demonstrated a significant reduction in the number of cell colonies after ZWINT interference, while Edu assay indicated a decrease in the percentage of Edu-positive cells. Transwell assay suggested that cell migration and invasion were also affected by ZWINT interference. Conclusion: ZWINT was highly expressed in ovarian cancer tissues, and could promote the proliferation, migration and invasion of cancer cells.