Zur Serostruktur der O-Antigene von Pseudomonas aeruginosa

Müller, Hans‐Peter; Kleinmaier, H

doi:10.1007/bf02150043

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…Agar diffusion test by the Ouchterlony plate method. Pure agar (1.5%) (Behringwerke, Marburg, Federal Republic of Germany) with a supplement of 1:10,000 merthiolate served as the diffusion medium (41).…”

Section: Methodsmentioning

confidence: 99%

Variation and adaptation of Pseudomonas aeruginosa toxicity to HeLa cells and fibroblasts

Müller¹,

Kettelhack²,

Kettelhack³

et al. 1986

J Clin Microbiol

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The toxic components of supernatants from Pseudomonas aeruginosa cultures directed against HeLa cells and Staphylococcus aureus were evaluated with the aim of discovering interactions. Supernatants of eight different strains of P. aeruginosa were assayed for cytotoxic activity. All were active against HeLa cells; seven were toxic for S. aureus. On repeated suspension of P. aeruginosa in 0.9% sodium chloride solution, a shift from HeLa cell toxicity to staphylococcal lytic activity occurred along with a change of toxic activity from a high (50,000 + 5,000) to a low (8,000 + 400) molecular weight (MW) range on gel filtration. Addition of protein to the minimal medium of cultures producing material toxic only for S. aureus reactivated the generation of HeLa cell-toxic material. Cultivation of P. aeruginosa in the presence of HeLa cells and a chloramphenicol supplement produced suppression of the generation of material toxic for S. aureus but facilitated that of HeLa-toxic material of high MW. Adaptation of toxicity against fibroblasts developed only on cocultivation of P. aeruginosa together with S. aureus and in the presence of fibroblasts. Under these conditions a strong lytic activity for S. aureus appeared, even in the presence of chloramphenicol. Chloramphenicol caused the material toxic for fibroblasts to elute at a low MW well separated from that toxic for HeLa cells. In contrast to the high-MW toxic substances, the low-MW material did not induce antibodies after injection into rabbits. This may explain failures of vaccination against P. aeruginosa infection and of serum therapy of homologous sepsis in humans.

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Section: Methodsmentioning

confidence: 99%

Variation and adaptation of Pseudomonas aeruginosa toxicity to HeLa cells and fibroblasts

Müller¹,

Kettelhack²,

Kettelhack³

et al. 1986

J Clin Microbiol

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Chapter IV Serological Characterization of Pseudomonas aeruginosa

Lányi

Bergan

1978

Methods in Microbiology

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A Serological Approach to the Genus Pseudomonas

Hobbs

Cann

Gowland

et al. 1964

Journal of Applied Bacteriology

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Summary: Serological methods are described by which organisms of the genus Pseudomonas and related genera can be compared. More specific relationships are demonstrated by agglutination techniques or agar diffusion with trichloroacetic acid extracts; less specific relationships are shown by agar diffusion with disintegrated cell preparations. Up to three strain specific antigens may be found and at least six antigens are widely distributed amongst strains of the genera Pseudomonas, Achromobacter, Vibrio and Aeromonas. The results obtained so far indicate little or no agreement with any accepted taxo‐nomic divisions. Evidence is presented which suggests that most of the antigens detectable by agar diffusion techniques are polysaccharide in nature rather than protein.

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Zur Serostruktur der O-Antigene von Pseudomonas aeruginosa

Cited by 4 publications

References 8 publications

Variation and adaptation of Pseudomonas aeruginosa toxicity to HeLa cells and fibroblasts

Variation and adaptation of Pseudomonas aeruginosa toxicity to HeLa cells and fibroblasts

Chapter IV Serological Characterization of Pseudomonas aeruginosa

A Serological Approach to the Genus Pseudomonas

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