Zur Isolierung und Kultur insektenpathogener Entomophthoraceen

Müller-Kögler, E.

doi:10.1007/bf02374882

Cited by 39 publications

(6 citation statements)

References 13 publications

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“…Few EFOPSZ fungi can be cultivated on typical fungal nutritional media such as malt extract or potato dextrose agar or in the corresponding liquid media [ 168 ]. In the past, to ensure the fungal growth of entomopathogens, special media containing animal protein from additives such as liver, extracts of fresh or dried insects, blood serum, or egg yolk were used [ 23 , 169 ], providing the pathogens with specific nutrients absent in the usual laboratory media. Sometimes rare and exotic media components such as fly fat bodies are used to stimulate spore germination or hyphal growth.…”

Section: Resultsmentioning

confidence: 99%

Potential for Use of Species in the Subfamily Erynioideae for Biological Control and Biotechnology

Gryganskyi,

Hajek,

Voloshchuk

et al. 2024

Microorganisms

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The fungal order Entomophthorales in the Zoopagomycota includes many fungal pathogens of arthropods. This review explores six genera in the subfamily Erynioideae within the family Entomophthoraceae, namely, Erynia, Furia, Orthomyces, Pandora, Strongwellsea, and Zoophthora. This is the largest subfamily in the Entomophthorales, including 126 described species. The species diversity, global distribution, and host range of this subfamily are summarized. Relatively few taxa are geographically widespread, and few have broad host ranges, which contrasts with many species with single reports from one location and one host species. The insect orders infected by the greatest numbers of species are the Diptera and Hemiptera. Across the subfamily, relatively few species have been cultivated in vitro, and those that have require more specialized media than many other fungi. Given their potential to attack arthropods and their position in the fungal evolutionary tree, we discuss which species might be adopted for biological control purposes or biotechnological innovations. Current challenges in the implementation of these species in biotechnology include the limited ability or difficulty in culturing many in vitro, a correlated paucity of genomic resources, and considerations regarding the host ranges of different species.

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Section: Resultsmentioning

confidence: 99%

Potential for Use of Species in the Subfamily Erynioideae for Biological Control and Biotechnology

Gryganskyi,

Hajek,

Voloshchuk

et al. 2024

Microorganisms

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“…Culture tubes were prepared by placing 10 ml aliquots of heated Sabouraud's maltose and dextrose agars into 15x150 mm tubes, which were then stoppered with eotton plugs, sterilized 15 minutes und er 10 pounds pressure at 115 "C, and cooled into slants. The egg yolk was prepared according to Müller-Kögler (1959).…”

Section: Methodsmentioning

confidence: 99%

Four new species of Zeytinus Doğanlar, 2011 (Hymenoptera: Eulophidae: Tetrastichinae) from Turkey and a redescription of the type species, Zeytinus hatayensis Doğanlar, 2011

Doğanlar¹,

Sakin

Laz

2020

Türkiye Biyolojik Mücadele Dergisi

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Türkiye'den Zeytinus Doğanlar, 2011 (Hymenoptera: Eulophidae: Tetrastichinae) Cinsine giren dört yeni türün tanılanmaları ve cinsin tip türü, Zeytinus hatayensis Doğanlar, 2011'in yeniden tanılanması Öz: Bu makale Zeytinus hatayensis Doğanlar, 2011'in yeniden tanılanması ile 4 yeni türün ve bu türlerin bazı biyolojik özelliklerinin açıklamasını içermektedir. Türkiye için, Zeytinus Doğanlar cinsine bağlı 4 yeni tür olan; Z. edremitensis Doğanlar and Sakin, n.sp., Z. balikesirensis Doğanlar and Sakin, n. sp., Z. marmarae Doğanlar and Sakin, n. sp. ve Z. alyssumus Doğanlar and Laz (Hym.: Eulophidae) tanımlanması yapılmıştır. Bu türlerden ilk üçü Samandağ (Hatay) ve Edremit (Balıkesir) ilçelerinde, zeytin ağacı (Olea europea L.) yapraklarında zararlı olan Zeytin Gal sineği, Dasineura oleae (Loew) (Dipt.: Cecidomyiidae)'nin larva/pupa parazitoitleri olarak elde edilmiştir. Sonuncu tür ise Kahramanmaraş ilinde bulunan Alyssum pateri ssp. pateri Nyár (Brassicaceae) bitkisinin çiçekleri üzerinde beslenen bir Dasineura türünün gallerinden üretilmiştir.

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“…Culture of microorganism: The strain Zooplzfhora (Erynia) ouispora, used in these studies, was maintained on medium recommended by MULLER- KOGLER (1959): BACTO peptone 15 g, yeast extract 3 g, 12 egg yolks, bacto agar 30 g in 1 1 of distilled water at pH = 7.0. The liquid medium, used in the larger growth scale, had the following composition: glucose 20 g, bacto peptone 10 g, yeast extract 2 g, caseine hydrolyzate 2 g, NaCl6 g in 1 1 of distilled water at pH = 7.0.…”

Section: Methodsmentioning

confidence: 99%

Neutral lipids of the fungus Zoophthora (Erynia) ovispora (NOWAKOWSKI) BATKO

Urbańczyk

Zabźza

Cichacz

et al. 1992

J. Basic Microbiol.

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The main components of the neutral lipid fractions, isolated from Zoophthora (Erjmia) ovispora, were determined. Triglycerides constitute 56% of total lipids. among them dilaurinostearate glyceride constitutes 43.3%. The major fatty acid -lauric acid (32.3%) is related mainly to tri-and diglycerides. The level of stearic acid is slightly lower (29.8%) but distinctly predominates in free acid fraction. Diglycerides and steroids were present in smaller amounts -1.2% and 4.8%, respectively. Cholesterol exceeds considerably ergosterol.Small differences in morphology and specificity of fungi belonging to the Zoophthera genus cause many problems in their taxonomy (BALAZY 1986). In previous publication we described our attempts to utilize an enzymatic activity of selected fungi of the Z. Zoophthora, Z. Erjwia and Z. Neoparidora subgenus as criteria in their taxonomy. However, observed lipolytic, chitinolytic, proteolytic and amylolytic activities were not enough specific to be crucial argument in their classification (URBANCZYK et al. 1992). Therefore, we decided to perform analysis of the neutral lipid fraction isolated from one fungus species of Zoophthora genus and to compare it with lipids isolated from other fungi of the Entomophthoraceae family. The compositions of fatty acids were often utilized in the chemotaxonomy of microorganisms by many authors (TYRREL 1967, LATGE and DE BIEVRE 1980, MUMMA and In this paper we describe the components of the neutral lipid fractions isolated from the Zoophthora (Erynia) ouisporu (NOWAKOWSKI) BATKO. This microorganism has been deposited (under no 1854) in the mycological collection of the Research Institute of Agricultural and Forest Environment of the Polish Academy of Sciences in Poznan. The Z. (Erynia) ovispora (NOWAKOWSKI) BATKO shows the ability to infect nomatocerous, brachycerous and calyptrate flies (hitherto found on insects belonging to families : Culicidae, Linioniidae, Psychodidae, Tendipedidue, Tipulidae, Syrphidae, Sarcophagidue and Calliphoridae). Therefore, this fungus should be considered as a widely oligophagous species. Because its laboratory cultivation is not too difficult (URBANCZYK et a/. 1992). This microorganism could be considered as a potential biolgical insecticide.Contribution of particular components in the lipid fraction depends on time of microorganism cultivation. In separate experiments we found that the greater increase of mycelium mass was observed between 6 and 7 day (Fig. 1). On the other hand, the greatest amounts of the neutral lipid fractions were extracted from the cultures of 10 days old. We have also found that at this time the composition of lipids was also the most diversified (Fig. 2). Therefore, we decided to take to our studies the lipid fractions produced by this microorganism in 10th day of culturing.

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Zur Isolierung und Kultur insektenpathogener Entomophthoraceen

Cited by 39 publications

References 13 publications

Potential for Use of Species in the Subfamily Erynioideae for Biological Control and Biotechnology

Potential for Use of Species in the Subfamily Erynioideae for Biological Control and Biotechnology

Four new species of Zeytinus Doğanlar, 2011 (Hymenoptera: Eulophidae: Tetrastichinae) from Turkey and a redescription of the type species, Zeytinus hatayensis Doğanlar, 2011

Neutral lipids of the fungus Zoophthora (Erynia) ovispora (NOWAKOWSKI) BATKO

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