Zur Histochemie des Zinks

Timm, F.

doi:10.1007/bf00664118

Cited by 49 publications

(12 citation statements)

References 3 publications

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“…The autometallographic method used in this study is based on exposure of the tissue to sulphide ions (Timm, 1958;Danscher, 1996). Loosely bound or free zinc ions will then be chemically bound in situ as nanometer-sized zinc sulphide crystals.…”

Section: Discussionmentioning

confidence: 99%

Histochemical tracing of zinc ions in the rat testis

Sörensen

Stoltenberg²,

Henriksén³

et al. 1998

Molecular Human Reproduction

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To detect free zinc ions in the rat testes four rats were transcardially perfused with Na 2 S, and the seminiferous tubules from two other rats were incubated in Na 2 S. Sections from the two sources were autometallographically (AMG) developed, whereby zinc sulphide crystal lattices created in the tissue by the sulphide treatment were silver enhanced. Light microscopical analysis showed zinc ions in primary spermatogonia until the zygotene primary spermatocytes (stage I), in late pachytene spermatocytes (stages XII and XIII), and in late spermatids from step 15 to step 19 (stages I-VIII). The highest intensity of AMG grains was detected in the residual bodies and tails of step 19 spermatids. Grains were occasionally found in the cytoplasm of Leydig cells. Sections from animals treated with the chelator diethyldithiocarbamate prior to sulphide treatment showed a complete lack of AMG staining. At ultrastructural levels the AMG grains were found in smooth-surfaced endoplasmic reticulum of all spermatogonial stages, and in the acrosome, midpiece, and tail of late spermatids. The presence of zinc ions in preleptotene spermatocytes and cytoplasmic lobes of late spermatids suggests a specific role of free zinc at the onset of meiosis and at spermiation.

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Section: Discussionmentioning

confidence: 99%

Histochemical tracing of zinc ions in the rat testis

Sörensen

Stoltenberg²,

Henriksén³

et al. 1998

Molecular Human Reproduction

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“…silver-amplified metal sulphide aggregations, in sections from blocks of brain tissue that had been exposed to a fixative containing sulphide ion (Timm, 1958). The silver grains were produced by developing the sections with Liesegang's developer (Liesegang, 1911).…”

Section: Introductionmentioning

confidence: 99%

The autometallographic zinc-sulphide method. A new approach involvingin vivo creation of nanometer-sized zinc sulphide crystal lattices in zinc-enriched synaptic and secretory vesicles

Danscher

1996

Histochem J

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A new version of Timm's sulphide silver method involving in vivo binding of zinc ions in zinc enriched terminals is presented. By injecting sodium sulphide into the vena cava of deeply anaesthetized animals, it is possible to bind chemically the vesicular zinc, i.e. chelatable zinc (zinc ions), in secretory and synaptic vesicles, in the form of zinc sulphide crystal lattices. Four minutes after the intravenous injection the animal is perfused transcardially with a phosphate-buffered solution of glutaraldehyde, glutaraldehyde and formaldehyde, or with a saline solution. The nanometer-sized catalytic crystals can then be silver-amplified in cryostat and vibratome sections by exposure to an autometallographic developer. It is demonstrated that contemporaneously with silver enhancement, the zinc sulphide crystals are transformed to the corresponding silver sulphide crystals. For ultrastructural studies, autometallographic development of vibratome sections is recommended. From these sections tissue blocks are cut from the areas of interest, blockstained with osmium tetroxide and embedded in Epon. This approach results in a zinc-specific autometallographic staining of the sections of a hitherto unseen, high technical quality.

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“…1959] and J. A.Shevtchuk [1963,1964], in which the histochemical method of zinc determination (silver-sulphide method of Timm) (Timm [1958]) has been used for revealing the islet cells. In view of the close connection of zinc with the insulin mole cule the use of this method for the study of the islets of Langerhans and their cells offers particular interest.…”

Section: I F a U Nmentioning

confidence: 99%

The development and cytodifferentiation of the islets of Langerhans in human embryos and foetuses

Falin¹

1967

Cells Tissues Organs

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Zur Histochemie des Zinks

Cited by 49 publications

References 3 publications

Histochemical tracing of zinc ions in the rat testis

Histochemical tracing of zinc ions in the rat testis

The autometallographic zinc-sulphide method. A new approach involvingin vivo creation of nanometer-sized zinc sulphide crystal lattices in zinc-enriched synaptic and secretory vesicles

The development and cytodifferentiation of the islets of Langerhans in human embryos and foetuses

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