Zur Frage der Natur der extremit�teninduzierenden Wirkung

Balinsky, B. I.

doi:10.1007/bf00576701

Cited by 11 publications

(1 citation statement)

References 29 publications

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“…On another front, it has long been reported that limb induction occurs when ear vesicles (Balinsky, 1925) or nose placodes (Balinsky, 1937(Balinsky, , 1956 are transplanted into newt embryo flank regions, suggesting the presence of a limbinducing substance common to such heterotopic inducers. An experiment with chick embryos has shown that the fibroblast growth factors (FGFs) members might be potential inducers of limb bud initiation; when FGF-soaked beads were implanted into the flank regions of chicken embryos, extra limbs developed (Cohn et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Formation of a New Limb Bud at the Boundary Between a Transplanted Limb Bud and the Tail Surface ofXenopusTadpoles

Adaniya

Nishikawa

2015

Zoological Science

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Through transplantation experiments with Xenopus laevis tadpoles, we found a new morphogenetic phenomenon consisting of limb bud formation at the boundary between transplanted whole limb buds and the tail surface. This phenomenon occurs without limb-limb stump interaction and has a number of unique features: (1) Only one extra limb bud was formed per transplant and the new limb and the transplanted limb were bilaterally symmetrical, forming a pair of limb girdles. (2) Extra new limb bud formation occurred not only at the tail but also at other non-limb regions, including abdominal and head surfaces. (3) Successful limb formation required the presence of basal 1/4 region (presumptive limb girdle) of a limb bud explant. (4) New limb formation was host-stage-dependent: before metamorphosis, limb bud formation ratio was high (> 90%), but as the host tadpole entered metamorphosis, this potential declined and morphological abnormalities of new limbs increased. (5) Cell lineage analysis showed that epidermis of the new limb bud always contained many (about 60%) host-derived cells, while new limb cartilage cells were completely graft-derived. These results suggest that heterotopic new limb formation occurs through interaction between graft mesenchyme and host epidermis. Thus, the present study has clarified the two important aspects of limb ontogeny: the importance of presumptive limb girdle for the limb bud initiation and the relationship between limb bud formation potential and metamorphic tissue remodeling. The present experimental system may help to improve our understanding of epithelial-mesenchymal interaction during limb bud initiation and subsequent limb cell differentiation during metamorphosis.

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Section: Introductionmentioning

confidence: 99%

Formation of a New Limb Bud at the Boundary Between a Transplanted Limb Bud and the Tail Surface ofXenopusTadpoles

Adaniya

Nishikawa

2015

Zoological Science

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New experiments on the mode of action of the limb inductor

1957

J. Exp. Zool.

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Supernumerary limb induction in the anura

Balinsky

1974

J. Exp. Zool.

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Supernumerary Limb Induction in t h e A n u r a B. 1. BALINSKY D e p u r t m e n t of Zoology, Llniucrsity of the Witzutrtcrsrtr 1 1~1 , Johrlizn rsburg ABSTRACTInduction of supcrnumcrary limbs in Bufo tadpoles was achieved by transplanting the transverse rieural fold with adjoining tissue €rom neurulae into tail bud stage embryos. The graft differentiatcd into brain, eyes, noses and mouthparts. Induced limb rudiments were associatcd with either noses or mouthparts. l'he author rejects the suggestion made by Perri that the induction is due to release of products of cytoolysis froin the graft and suggests that limb induction is due to a disorganization or reduction of the subepidermal basement imcmbrane (layer of collagen fibres) by certain grafts, and that as a result the potentially limb forming flank mesenchyme is allowed to interact with the epidermis -a precondition for limb development. In support of this view the author prvscnts clectronmicrographs in which it is shown that at the edge of the nostril, arid on the mouthparts (lips, oral papillae), the basal membrane is attenuated and disorganised, allowing processes of mesenchyme cells to penetrate through the layer of collagen fibres and reach the inner surface 01 the epidermis.

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Zur Frage der Natur der extremit�teninduzierenden Wirkung

Cited by 11 publications

References 29 publications

Formation of a New Limb Bud at the Boundary Between a Transplanted Limb Bud and the Tail Surface ofXenopusTadpoles

Formation of a New Limb Bud at the Boundary Between a Transplanted Limb Bud and the Tail Surface ofXenopusTadpoles

New experiments on the mode of action of the limb inductor

Supernumerary limb induction in the anura

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