Zur Bestimmung der Phosphomonoesterasen in Rohmilch

Influence of storage on enzymes in rice grains

“…Determination of Peroxidase. Peroxidase was determined by measuring the oxidation of p-phenylenediamine as a substrate at 452 nm (Kiermeier and Kayser, 1960; Kiermeier and Meinl, 1961). The incubation mixture contained 0.1 mL of 3 X 10~3 M p-phenylenediamine, 0.1 mL of 6 X -2 M H202, 2.3 mL of 0.2 M Tris buffer (pH 7.5), and 0.5 mL of enzymic rice flour extract.…”