Zur Ätiologie einer unbekannten, von Affen ausgegangenen menschlichen Infektionskrankheit

Siegert, R.; Shu, Hsin-Lu; Slenczka, Werner; Peters, D.; Müller, G.

doi:10.1055/s-0028-1106144

Cited by 190 publications

(83 citation statements)

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“…Next we expanded the analysis of filoviral GP 2 subunits to Marburg virus (MARV) and Lloviu cuevavirus (LLOV), the only two remaining genera of Filoviridae 47,48 . Comparisons of the TMDs of the respective GP 2 subunits of these viruses ( Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol

et al. 2015

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The high pathogenicity of the Ebola virus reflects multiple concurrent processes on infection. Among other important determinants, Ebola fusogenic glycoprotein (GP) has been associated with the detachment of infected cells and eventually leads to vascular leakage and haemorrhagic fever. Here we report that the membrane-anchored GP is sufficient to induce the detachment of adherent cells. The results show that the detachment induced through either full-length GP 1,2 or the subunit GP 2 depends on cholesterol and the structure of the transmembrane domain. These data reveal a novel molecular mechanism in which GP regulates Ebola virus assembly and suggest that cholesterol-reducing agents could be useful as therapeutics to counteract GP-mediated cell detachment.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol

et al. 2015

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“…Infection of humans or monkeys with MBGV resulted in a systemic infection affecting, among others, several organs with polarized cells like the liver, kidney, and lung (33,39). The role of vectorial budding from these polarized cells in the course of MBGV disease is poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…Several attempts have been made to detect the natural reservoir of filoviruses with no success (5,19). The prototype of Filoviridae, MBGV, was isolated in 1967, when several laboratory workers were infected after contact with imported monkeys (39).…”

Section: Marburg Virus (Mbgv) and The Closely Related Ebola Virusmentioning

confidence: 99%

Sorting of Marburg Virus Surface Protein and Virus Release Take Place at Opposite Surfaces of Infected Polarized Epithelial Cells

Sänger

Mühlberger

Ryabchikova

et al. 2001

J Virol

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Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP permanently (MDCK-GP). The functional integrity of GP expressed in these cells was analyzed using vesicular stomatitis virus pseudotypes. Further experiments revealed that GP is transported in MDCK-GP cells mainly to the apical membrane and is released exclusively into the culture medium facing the apical membrane. When MDCKII cells were infected with Marburg virus, the majority of GP was also transported to the apical membrane, suggesting that the protein contains an autonomous apical transport signal. Release of infectious progeny virions, however, took place exclusively at the basolateral membrane of the cells. Thus, vectorial budding of Marburg virus is presumably determined by factors other than the surface protein.

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“…All experiments were conducted using the E6 strain of Vero cells (McCormick et al, 1982). MBG virus strains designated by location and year of isolation and original reference were: RYC and Voege (VOG) (Germany/Uganda, 1967) (Siegert et al, 1967), OZO and Hogan (HGN) (South Africa, 1975) (Gear et al, 1975) and Musoke (MUS) (Kenya, 1980) (Morbidity and Mortality Weekly Report, 1980). EBO and MBG viruses were isolated from clinical materials in Vero cells.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical Properties of Marburg Virus: Evidence for Three Distinct Virus Strains and Their Relationship to Ebola Virus

Kiley

Cox

Elliott

et al. 1988

Journal of General Virology

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SUMMARYThe physicochemical and antigenic properties of three groups of Marburg (MBG) virus isolates, separated temporally and geographically, were compared to each other and to another member of the same family, Ebola (EBO) virus. Each MBG isolate contained seven virion proteins, one of which was a glycosylated surface protein.Peptide mapping of glycoproteins, nucleoproteins (NP) and viral structural protein (VP40) demonstrated extensive sequence conservation in the proteins of viruses isolated over a 13-year period, but homology was not evident in VP24. Some homology between the NPs of MBG and EBO was observed. A close antigenic relationship between MBG strains was found by radioimmunoassay but no evidence was found of antigenic cross-reactivity with EBO viruses. MBG virion proteins are produced from virus-specific monocistronic mRNA species. Five of the seven viral proteins were produced by in vitro translation of these RNAs. MBG virions contained one RNA species with an Mr of 4.2 x 106 and virions had a density of 1.14 g/ml in potassium tartrate. Virus isolates from different outbreaks had distinct TI oligonucleotide maps, but had approximately 9570 homology in base sequence. No two geographically distinct virus pairs were more closely related to each other than to a third virus isolate. MBG viruses are thus similar to EBO viruses in morphology and other physicochemical properties and are very similar to each other in RNA and protein composition. Each of the three geographically and temporally distinct MBG virus outbreaks appears to have been due to a genetically distinguishable, but antigenically closely related virus strain. In addition, these studies confirm the belief that MBG and EBO viruses are members of the new virus family, the Filoviridae.

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Zur Ätiologie einer unbekannten, von Affen ausgegangenen menschlichen Infektionskrankheit

Cited by 190 publications

References 0 publications

Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol

Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol

Sorting of Marburg Virus Surface Protein and Virus Release Take Place at Opposite Surfaces of Infected Polarized Epithelial Cells

Physicochemical Properties of Marburg Virus: Evidence for Three Distinct Virus Strains and Their Relationship to Ebola Virus

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