Zuogui Wan alleviated maternal kidney-yin deficiency-induced thymic epithelial cell dysfunction in newborn rats through Wnt/β-catenin signaling pathway

Chen, Longyun; Ren, Man; Cao, Jigang; Sang, Hongling; Chen, Huimin; Xu, Anlong; Zhao, Min

doi:10.1016/j.jep.2021.114337

Cited by 3 publications

(3 citation statements)

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“…DN with yin and yang deficiency is an important subject of pathogenesis throughout the course of disease ( Lu et al, 2004 ). Kidney-yin deficiency results in the dysregulation of lipid and glucose metabolism ( Chen et al, 2021 ). ZGW, a classic traditional medicine to treat kidney-yin deficiency, has been described in Jingyue Quanshu by Jingyue Zhang (1563–1640 A.D.).…”

Section: Discussionmentioning

confidence: 99%

“…ZGW is also indicated in the Chinese Pharmacopoeia for treating kidney-yin deficiency and associated with glycemic control in gestational diabetes ( Xu et al, 2019 ). Meanwhile, the medicine alleviated maternal kidney-yin deficiency-induced thymic epithelial cell dysfunction ( Chen et al, 2021 ). Protecting podocytes against hyperglycemia can prevent diabetes-associated albuminuria without the need of restoring normal levels of glucose.…”

Section: Discussionmentioning

confidence: 99%

“…(B) Necrotic cell death was assessed by the release of LDH after 24 h treatment with Zuogui pill. (C) Podocytes were preincubated with Zuogui pill by 100, 300, and 1,000 μg/ml for 1 h then exposed to high glucose ( 30 Frontiers in Pharmacology frontiersin.org deficiency results in the dysregulation of lipid and glucose metabolism (Chen et al, 2021). ZGW, a classic traditional medicine to treat kidney-yin deficiency, has been described in Jingyue Quanshu by Jingyue Zhang (1563-1640 A.D.).…”

Section: Figurementioning

confidence: 99%

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Zuogui Wan ameliorates high glucose-induced podocyte apoptosis and improves diabetic nephropathy in db/db mice

Zhu

Ji²,

Ju³

et al. 2022

Front. Pharmacol.

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Zuogui Wan (ZGW), a well-known traditional Chinese medicine (TCM), has been used to nourish “Kidney-Yin” for a long time in China, implying a protective effect on the kidney. The aim of the present study is to investigate the effect of ZGW on high glucose-induced podocyte apoptosis and diabetic nephropathy (DN) in db/db mice. ZGW (1 g/kg−1/day−1) was administered intragastrically to db/db mice for 8 weeks. HPLC was used for identifying the components of ZGW, biochemical and histopathological approaches were used for evaluating its therapeutic effects, and cultured mouse podocytes were used for further exploring its underlying mechanism in vitro. ZGW improved renal function and podocyte loss and also normalized kidney reactive oxygen species production in db/db mice. The cytotoxicity of ZGW on mouse podocytes was assessed by the LDH assay. The effect of ZGW on podocyte viability and apoptosis was determined with CCK-8 and Annexin-V/PI staining by treatment with high glucose. ZGW attenuated podocyte apoptosis, and oxidative stress was detected by the peroxide-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) staining in a dose-dependent manner. Furthermore, ZGW decreased the expression of caspase-3 and phospho-p38 in both the kidney cortex and high glucose-treated podocytes. Thus, our data from in vivo and in vitro studies demonstrated that ZGW improved renal injury in diabetes by inhibiting oxidative stress and podocyte apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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