Zuogui Pills inhibit mitochondria-dependent apoptosis of follicles in a rat model of premature ovarian failure

Peng, Huijuan; Zeng, Lihua; Zhu, Ling; Luo, Songping; Xu, Liping; Zeng, Lingyu; Li, Jing; Liang, Qunying; Geng, Hongling

doi:10.1016/j.jep.2019.111855

Cited by 32 publications

(20 citation statements)

References 26 publications

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“…As previously reported, CTX was shown to be detrimental to follicle development by inducing apoptosis of granulosa cells, disturbing the indispensable environment for oocyte maturation, and finally causing prolonged and severe damage to ovaries (Song et al, 2016;Pascuali et al, 2018;Peng et al, 2019). Consistent with these studies, we found that CTX seriously affected ovarian structure and function by promoting excessive activation and subsequent deletion of oocytes with decreased primary or secondary follicles and disrupted estrous cycles (Fu et al, 2017;Hou et al, 2018).…”

Section: Discussionsupporting

confidence: 90%

Study on the Reparative Effect of PEGylated Growth Hormone on Ovarian Parameters and Mitochondrial Function of Oocytes From Rats With Premature Ovarian Insufficiency

Feng

Xie

Liu

et al. 2021

Front. Cell Dev. Biol.

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Premature ovarian insufficiency (POI) is a heterogeneous disorder and lacks effective interventions in clinical applications. This research aimed to elucidate the potential effects of recombinant human PEGylated growth hormone (rhGH) on follicular development and mitochondrial function in oocytes as well as ovarian parameters in POI rats induced by the chemotherapeutic agent. The impacts of rhGH on ovarian function before superovulation on follicles, estrous cycle, and sex hormones were evaluated. Oocytes were retrieved to determine oocyte quality and oxidative stress parameters. Single-cell sequencing was applied to investigate the latent regulatory network. This study provides new evidence that a high dosage of rhGH increased the number of retrieved oocytes even though it did not completely restore the disturbed estrous cycle and sex hormones. rhGH attenuated the apoptosis of granulosa cells and oxidative stress response caused by reactive oxygen species (ROS) and mitochondrial superoxide. Additionally, rhGH modulated the energy metabolism of oocytes concerning the mitochondrial membrane potential and ATP content but not mtDNA copy numbers. Based on single-cell transcriptomic analysis, we found that rhGH directly or indirectly promoted the balance of oxidative stress and cellular oxidant detoxification. Four hub genes, Pxmp4, Ehbp1, Mt-cyb, and Enpp6, were identified to be closely related to the repair process in oocytes as potential targets for POI treatment.

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Section: Discussionsupporting

confidence: 90%

Study on the Reparative Effect of PEGylated Growth Hormone on Ovarian Parameters and Mitochondrial Function of Oocytes From Rats With Premature Ovarian Insufficiency

Feng

Xie

Liu

et al. 2021

Front. Cell Dev. Biol.

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“…Cytochrome c and adenosine triphosphate form a complex and activate caspase-3. Peng et al [43] demonstrated the upregulation of Bax and cytochrome c in CP-induced POF rats. In this study, we found that RA promoted CP-induced apoptosis, which was accompanied by the downregulation of cytochrome c and caspase-3.…”

Section: Discussionmentioning

confidence: 99%

Protective roles and mechanisms of rosmarinic acid in cyclophosphamide‐induced premature ovarian failure

Yao

Wang

2020

J Biochem & Molecular Tox

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The aim of this study was to investigate the protective effect of rosmarinic acid (RA) in a premature ovarian failure (POF) mouse model and the potential mechanisms. The POF model was induced by a single intraperitoneal injection of 120 mg/kg cyclophosphamide (CP). Additionally, 40 mg/kg RA was administered for 7 days before CP injection. The concentration of sex hormones was determined by fluorescence immunohistochemistry. Histological analysis was performed after ovarian tissue sections were stained with hematoxylin and eosin. The expression of the NLRP3 inflammasome was examined by western blot analysis and polymerase chain reaction. The expression of apoptosis markers of cytochrome c and caspase-3 was also detected by western blot analysis and immunohistochemistry. The results showed that RA not only decreased the ovarian index in POF mice but also improved the abnormal secretion of reproductive hormones associated with POF. Treatment with RA suppressed the ovarian expression of the NLRP3 inflammasome and regulated the ovarian expression of apoptosis-related proteins. The results suggested that RA exhibited a protective effect against CP-induced POF potentially by suppressing apoptosis and the NLRP3 inflammasome.

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“…ZGP resulted in a significant decrease in serum follicle-stimulating hormone (FSH) concentration and an increase in estradiol (E2). In addition, after administration of ZGP, a significant downregulation of Bax and cytochrome c (Cyt-c) and an upregulation of B-cell lymphoma/leukemia 2 (Bcl-2) were observed at both the gene and protein levels; thus, it is assumed that the effect may be achieved by inhibiting mitochondrial-dependent apoptosis in follicles [35]. Moreover, Bushen Shugan decoction, Yulinzhu, Guilu Erxian decoction, Bushen Tiaojing decoction, and Bazhen decoction have been also proven to have therapeutic effects on POF (Tables 1-3) [36][37][38][39][40][41][42][43][44].…”

Section: Compound Chinese Medicinementioning

confidence: 99%

The Treatment of Complementary and Alternative Medicine on Premature Ovarian Failure

Lin

Jia

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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It has been confirmed by growing evidence that common hormone replacement therapy is associated with an increasing risk of causing cardiovascular disease and cancer, while complementary and alternative medicine (CAM) is gaining popularity and application in more and more patients with premature ovarian failure (POF). Although there is little data concerning the clinical safety and efficacy of CAM, the literature includes application studies on the phytoestrogen-rich herbal, acupuncture treatment and intervention therapy. This article reviews recent literature on CAM therapy for POF, aiming to provide theoretical support for clinical application.

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Zuogui Pills inhibit mitochondria-dependent apoptosis of follicles in a rat model of premature ovarian failure

Cited by 32 publications

References 26 publications

Study on the Reparative Effect of PEGylated Growth Hormone on Ovarian Parameters and Mitochondrial Function of Oocytes From Rats With Premature Ovarian Insufficiency

Study on the Reparative Effect of PEGylated Growth Hormone on Ovarian Parameters and Mitochondrial Function of Oocytes From Rats With Premature Ovarian Insufficiency

Protective roles and mechanisms of rosmarinic acid in cyclophosphamide‐induced premature ovarian failure

The Treatment of Complementary and Alternative Medicine on Premature Ovarian Failure

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