Zum Nachweis von Aminen im 10−10-Mol-Maßstab. Trennung von 1-Dimethylamino-naphthalin-5-sulfonsäure-amiden auf Dünnschichtchromatogrammen

Seiler, Nikolaus; Wiechmann, Manfred

doi:10.1007/bf02141885

Cited by 64 publications

(4 citation statements)

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“…These studies involved n-butanol extraction, thin-layer chromatography, and visualization by ninhydrin (28). Later (6), a more sensitive procedure was used: dansyl chloride complexes of polyamines in cell extracts were chromatographed and visualized under UV light (20,21,60). Spermine again could not be detected in bloodstream T. 6 .…”

Section: Polyamine Content Of Trypanosomatidsmentioning

confidence: 99%

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1^,2

Bacchi

1981

The Journal of Protozoology

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Polyamines are multiply amine-substituted straight-chain aliphatics; their content in different tissues may vary widely, and their functions are many. Their main routes of biosynthesis originate from ornithine and methionine. Polyamine content and biosynthesis in tryposomatid flagellates are reviewed concluding with emphasis on their possible role as critical drug targets in these parasitic protozoa so pathogenic for man in large areas of the world.

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Section: Polyamine Content Of Trypanosomatidsmentioning

confidence: 99%

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1^,2

Bacchi

1981

The Journal of Protozoology

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“…Analysis of Polyamines. Polyamines were analyzed by the procedure described by Seiler and Wiechmann (22). Orange peel discs were extracted with 4% HC104, dansylated, and separated by TLC, using the solvent system ethylacetate:cyclohexane (2:3, v/v).…”

mentioning

confidence: 99%

Inhibition of Ethylene Biosynthesis by Aminoethoxyvinylglycine and by Polyamines Shunts Label from 3,4-[¹⁴C]Methionine into Spermidine in Aged Orange Peel Discs

Even‐Chen¹,

Mattoo²,

Gören³

1982

Plant Physiol.

162

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The flux of radioactivity from 3,4-114Clmethionine into S-adenosyl_L-methionine (SAM), 1-aminocyclopropane-l-carboxylic acid (ACC), sperm- (11), and by wounded tissue of citrus fruit (12, 29) has been previously reported. The induction of ethylene formation by ABA and the inhibitory effect of AVG on its production in citrus bud culture and leaf explants were also studied (10, 21). In the present investigation, we have used orange peel tissue to study relative incorporation of radioactivity from 3,4-[14CJmethionine into SAM, ACC, spermine, and spermidine while inhibiting biosynthesis of ethylene from ACC by cobalt and phosphate ions. By specifically inhibiting the terminal step of ethylene biosynthesis (Fig. 1)

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“…Acid extraction followed by deproteinization provides a suitable starting material. Very early methods involved separation by thin layer chromatography of either the amines followed by detection with reagents such as ninhydrin (248) or of fluorescent derivatives formed by dansylation (249, 250) or use of an amino acid analyzer with modified elution to release the more basic polyamines (251). These have largely been replaced by HPLC separation, which can be carried out with either postcolumn derivatization using o -phthalaldehyde (252) or by precolumn derivatization to form dansyl or benzoyl- or other readily quantifiable derivatives (253, 254).…”

Section: Chemical Properties and Analysis Of Polyaminesmentioning

confidence: 99%

Current Status of the Polyamine Research Field

Pegg

Casero

2011

Methods in Molecular Biology

202

175

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This chapter provides an overview of the polyamine field and introduces the 32 other chapters that make up this volume. These chapters provide a wide range of methods, advice, and background relevant to studies of the function of polyamines, the regulation of their content, their role in disease, and the therapeutic potential of drugs targeting polyamine content and function. The methodology provided in this new volume will enable laboratories already working in this area to expand their experimental techniques and facilitate the entry of additional workers into this rapidly expanding field.

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Zum Nachweis von Aminen im 10−10-Mol-Maßstab. Trennung von 1-Dimethylamino-naphthalin-5-sulfonsäure-amiden auf Dünnschichtchromatogrammen

Cited by 64 publications

References 3 publications

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1^,2

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1^,2

Inhibition of Ethylene Biosynthesis by Aminoethoxyvinylglycine and by Polyamines Shunts Label from 3,4-[¹⁴C]Methionine into Spermidine in Aged Orange Peel Discs

Current Status of the Polyamine Research Field

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Zum Nachweis von Aminen im 10−10-Mol-Maßstab. Trennung von 1-Dimethylamino-naphthalin-5-sulfonsäure-amiden auf Dünnschichtchromatogrammen

Cited by 64 publications

References 3 publications

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1,2

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1,2

Inhibition of Ethylene Biosynthesis by Aminoethoxyvinylglycine and by Polyamines Shunts Label from 3,4-[14C]Methionine into Spermidine in Aged Orange Peel Discs

Current Status of the Polyamine Research Field

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Product

Resources

About

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1^,2

Content, Synthesis, and Function of Polyamines in Trypanosomatids: Relationship to Chemotherapy1^,2

Inhibition of Ethylene Biosynthesis by Aminoethoxyvinylglycine and by Polyamines Shunts Label from 3,4-[¹⁴C]Methionine into Spermidine in Aged Orange Peel Discs