Zum Nachweis der Adenosintriphosphatase (ATPase) an in vitro gez�chteten Carcinomzellen

Gropp, A.; Hupe, K; Hellweg, H. R.

doi:10.1007/bf00678531

Cited by 15 publications

(2 citation statements)

References 5 publications

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“…Our observations on distribution of ATPase in all chick liver culture elements and on variability of the reaction for this enzyme, especially in the epithelial cells, appear to be in general agreement with those reported by different workers in reference to other types of cell cultures (2,23,28,42). The greater activity of the enzyme in the epithelial cells and macrophages than in the fibroblasts, noted by US, was also indicated by previous investigators.…”

Section: F Pheffects Discussionsupporting

confidence: 92%

Cytochemical Observations on Proteins, Alkaline and Acid Phosphatases, Adenosine Triphosphatase, and 5′‐Nucleotidase in Chick Liver Cell Cultures Infected with Trichomonas vaginalis*, †

Sharma

Honigberg

1967

The Journal of Protozoology

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SYNOPSIS. By means of the ninhydrin‐Schiff method for proteins a diffuse reaction as well as one localized in granular inclusions can be shown in the cytoplasm of fibroblasts, epithelial cells, and macrophages in trypsin‐dispersed chick liver cell cultures. Nuclei and nucleoli also take the specific stain. A progressive loss of cytoplasmic and nuclear staining occurs in the fibroblasts in cultures infected with a relatively pathogenic strain of T. vaginalis. A loss occurs in epithelial cells in advanced stages of degeneration, but in less damaged cells, while the diffuse reaction disappears, the number and staining intensity of the cytoplasmic inclusions remain unchanged or possibly may increase somewhat. The intensity of the diffuse reaction and the number and size of the characteristic inclusions increase in the active, parasite‐free, experimental macrophages, but phagocytes with trichomonads closely applied to their external surfaces and those containing the flagellates within their cytoplasm typically retain only a few weak‐staining inclusions. Similar distribution of alkaline and acid phosphatases occurs in preparations treated according to Gomori's and Burstone's methods, except that no nuclear staining is obtained with the latter. Activity of both enzymes is localized primarily in inclusions which are dispersed thruout the cytoplasm of fibroblasts and epithelial cells and tend to accumulate along the cell membranes and around the nuclei. In the course of infection with T. vaginalis there is a progressive loss of alkaline phosphatase from both cell types; however, the acid phosphatase activity increases. In the control macrophages both enzymes are localized in mostly rather large, rounded cytoplasmic inclusions. The number of such inclusions increases in the parasite‐free experimental macrophages, but only a few weak‐staining granules remainin phagocytes with engulfed trichomonads and in those whose external surfaces are in direct contact with the parasites. The loss of the inclusions is less apparent in macrophages containing degenerated flagellates than in the ones with healthy trichomonads, but regardless of the condition of the parasites, the highest enzymatic activity is found around them. ATPase and 5′‐nucleotidase are localized in small granules dispersed thruout the cytoplasm of fibroblasts and epithelial cells. The granules tend to accumulate along the periphery of the cells and around the nuclei. A diffuse cytoplasmic reaction is present in preparations processed for 5′‐nucleotidase. Nuclei and nucleoli give positive reactions for both enzymes. In the course of infection with trichomonads, activity of the 2 enzymes declines in both culture cell types. Control macrophages have diffuse cytoplasmic reaction for ATPase and 5′‐nucleotidase and these enzymes are localized also in rounded cytoplasmic inclusions. Activity of both enzymes increases in the parasite‐free experimental phagocytes, but little if any diffuse staining and only a few characteristic inclusions are left in macrophages with engulfed...

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Section: F Pheffects Discussionsupporting

confidence: 92%

Cytochemical Observations on Proteins, Alkaline and Acid Phosphatases, Adenosine Triphosphatase, and 5′‐Nucleotidase in Chick Liver Cell Cultures Infected with Trichomonas vaginalis*, †

Sharma

Honigberg

1967

The Journal of Protozoology

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“…The incorporation of cysteine into the incubation solution permitted the demonstration in HeLa cells of an ATPase other than non-specific alkaline phosphatase, which is inhibited by --SH groups (2). Gropp et al (3) showed that an --SH inbibitable surface ATPase and an --SH dependent cytoplasmic ATPase could be demonstrated in HeLa cells. In these experiments, cysteine hydrochloride (monohydrate) was used as the source of --SH groups at a concentration of 1.25 X 10 -3 M. Control experiments for HeLa cells were conducted by following each of the procedures as outlined above, with the exception that ATP or cysteine was omitted from the incubation solution.…”

Section: Inhibitorsmentioning

confidence: 99%

Cytochemical Localization of Adenosine Triphosphatase in the Mitotic Apparatus of Hela and Sarcoma 180 Tissue Culture Cells

Hartmann¹

1964

The Journal of Cell Biology

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ATPase was localized in distinct regions of the mitotic apparatus of HeLa and Sarcoma 180 tissue culture cells. ATPase was demonstrated in the metaphase spindle of HeLa and Sarcoma 180 cells fixed in cold buffered 2 per cent formalin (pH 6.5 to 6.8) containing 2 X l0 -a CaCl2. A high concentration of ATPase was frequently observed at the poles of the spindle. ATPase was also demonstrated in the interzonal region of both cell types during anaphase. The narrowing of the band of ATPase activity localized in the interzonal region during telophase indicates that ATPase activity is associated with the central spindle. In polar views of Sarcoma 180 cells fixed in cold, unbuffered, 2 per cent formalin, ATPasc was frequently localized in granules in the region of the inner circumference of the ring of chromosomes formed at metaphase. ATPase in the mitotic apparatus of HeLa and Sarcoma 180 cells was shown not to be due to non-specific alkaline phosphatase. Mitotic apparatus ATPase in Sarcoma 180 cells was suppressed by an --SH inhibitor.

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Der Einflu� verschiedener Aldehyde auf die Strukturerhaltung gez�chteter Zellen und auf die Darstellbarkeit von vier Phosphatasen

Hündgen

1968

Histochemie

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Zum Nachweis der Adenosintriphosphatase (ATPase) an in vitro gez�chteten Carcinomzellen

Cited by 15 publications

References 5 publications

Cytochemical Observations on Proteins, Alkaline and Acid Phosphatases, Adenosine Triphosphatase, and 5′‐Nucleotidase in Chick Liver Cell Cultures Infected with Trichomonas vaginalis*, †

Cytochemical Observations on Proteins, Alkaline and Acid Phosphatases, Adenosine Triphosphatase, and 5′‐Nucleotidase in Chick Liver Cell Cultures Infected with Trichomonas vaginalis*, †

Cytochemical Localization of Adenosine Triphosphatase in the Mitotic Apparatus of Hela and Sarcoma 180 Tissue Culture Cells

Der Einflu� verschiedener Aldehyde auf die Strukturerhaltung gez�chteter Zellen und auf die Darstellbarkeit von vier Phosphatasen

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