Zum Mechanismus der Proteinbiosynthese I. Die Bindung von Matrizen‐RNS und Aminoacyl‐RNS an Ribosomen

Matthaei, Heinrich; Amelunxen, F.; Eckert, Karin; Heller, G

doi:10.1002/bbpc.19640680811

Cited by 51 publications

(2 citation statements)

References 14 publications

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“…The 30S subunit is responsible for mRNAdependent binding of aa-tRNA ( 102,103). Since all effects of tRNA on EF-G GTPase activity are mRNA-dependent (83), presence of the 30S subunit is always required for tRNA to have an effect (69).…”

Section: B Action Of Mrna and Trnamentioning

confidence: 99%

Properties and regulation of the GTPase activities of elongation factors Tu and G, and of initiation factor 2

1981

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During protein synthesis the interaction with ribosomes of elongation factors Tu (EF-Tu), G (EF-G) and initiation factor 2 (IF-2) is associated with the hydrolysis of GTP which is directly related to the functions of the factors. In this article we review systematically the properties of these GTPase activities in the presence and absence of protein synthesis, and by examining the characteristics of the different minimal systems for the expression of these activities we point to the role of the various effectors and to the enzymological aspects of the systems. For EF-Tu, it has been possible to eliminate any requirement for macromolecular effectors, showing that the factor itself is a GTPase. For EF-G, the presence of at least the 50S ribosomal subunit has remained a requirement, whereas IF-2 needs both the 50S and 30S subunits to exhibit GTPase activity. Between the GTPase activities of the three factors there are some striking similarities, but important differences prevail as a consequence of the specificity of the different functions. This can also be seen by examining the respective ribosomal regions implicated in these reactions. When coupled with protein synthesis, the three GTPase activities reveal characteristics differing from those observed in partial systems.

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Section: B Action Of Mrna and Trnamentioning

confidence: 99%

Properties and regulation of the GTPase activities of elongation factors Tu and G, and of initiation factor 2

1981

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“…an isolated subunit. Isolated 30S subunits can bind messenger RNA (146) and aminoacyl tRNA (92,125). This latter step can be inhibited by tetracycline (92).…”

Section: Inhibition Of a Discrete Finction Associated Withmentioning

confidence: 99%