Zucchini Yellow Mosaic Virus in Three States of Australia.

Greber, R. S.; McLean, G. D.; Grice, Matt

doi:10.1071/app9870019

Cited by 25 publications

(11 citation statements)

References 9 publications

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“…Different working groups also reported opposing results. LECOQ and WIPF-SCHEIBEL (unpublished in LECOQ et al 2003) could not confirm any virus contamination in 70,000 seedlings from ZYMV-infected Cucurbitaceae of various cultivars (of different species) and ZYMV transmission was neither found in C. pepo (NAMETH et al 1985), Cucumis melo L. (GLEASON and PROVVI-DENTI 1990;WONG et al 1994), C. sativus, C. moschata (ROBIN-SON et al 1993) nor in C. maxima (GREBER et al 1987) seed. In contrast, DAVIS and MIZUKI (1986) found ZYMV seed transmission in seedlings of C. pepo, at a maximum rate of 80%.…”

Section: Discussionmentioning

confidence: 99%

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

et al. 2006

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In a survey in field-grown cucumber (Cucumis sativus L.) of the Spreewald cultivation area, Germany, from 2001 to 2004, infection with zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), cucumber mosaic virus (CMV) and cucumber green mottle mosaic virus (CGMMV) were recorded. ZYMV was of most relevance, owing to its damaging effects and frequency. Pumpkins (Cucurbita pepo L. and Cucurbita maxima Duchesne) were also found to be infected.In glasshouse experiments with 27 fresh and pickle cucumber cultivars frequently cultivated in the area, all cultivars proved (after mechanical inoculation) to be susceptible to ZYMV. ZYMV-infected cucumbers showed leaf chlorosis and mosaic, as well as deformation of leaves and fruits. Mechanical inoculation with ZYMV, in the early developmental stages of cucumber plants, reduced yields by up to 70%. Mixed infection with ZYMV and CMV resulted in synergistic effects, increasing the severity of damage. Fruit and leaf deformation also occurred in cucumber cultivars exhibiting a documented tolerance to CMV.The widespread weeds fat hen (Chenopodium album L.) and hoary alison (Berteroa incana (L.) DC.) were identified as possible sources of natural ZYMV infection in the Spreewald region. ZYMV transmission through seed seems to be of no importance, and possible only if fresh seeds from diseased cucumbers or pumpkins are used (commercially available seed is not contaminated). Uptake of ZYMV through contaminated soil is not a major mode of natural virus transmission, even though it proved possible in baiting experiments. Epidemiologic and economic implications of ZYMV infections of cucumber and pumpkin plants are discussed.In Untersuchungen zum Auftreten von Viren im Freilandanbau von Gurken im Spreewald wurden in den Jahren 2001 bis 2004 Zucchinigelbmosaikvirus (ZYMV), Wassermelonenmosaikvirus (WMV), Gurkenmosaikvirus (CMV) und das Grünscheckungsmosaikvirus der Gurke (CGMMV) nachgewiesen. Von besonderer Bedeutung erwies sich ZYMV im Untersuchungszeitraum durch sein häufiges Auftreten und die großen Schäden. Das Virus war sowohl in Gurken (Cucumis sativus L.) als auch in Kürbissen (Cucurbita pepo L. und Cucurbita maxima Duchesne) nachzuweisen.Versuche mittels mechanischer Inokulation ergaben für alle 27 geprüften Gurkensorten eine Anfälligkeit gegenüber ZYMV. Die Blätter ZYMV-infizierter Pflanzen zeigten Chlorosen und Mosaik, die Früchte waren deformiert. Pflanzen, die in frühen Entwicklungsstadien durch mechanische Inokulation infiziert wurden, reagierten mit Ertragsdepressionen von bis zu 70 Prozent. Bemerkenswert ist, dass eine Mischinfektion von ZYMV und CMV sichtbare Schäden an Sorten verursacht, welche als tolerant gegenüber CMV beschrieben werden.Auf der Suche nach möglichen Infektionsquellen von ZYMV in der Region Spreewald wurden 23 verschiedene Unkrautarten getestet. Natürliche Infektionen mit dem Virus wurden in Weißem Gänsefuß (Chenopodium album L.) und Graukresse (Berteroa incana (L.) DC) gefunden. Eine Übertragung von ZYMV durch kontaminiertes Saatgut wurde bis...

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Section: Discussionmentioning

confidence: 99%

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

et al. 2006

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“…Prieto, A. Bruna, P. Hinrichsen, and C. Muñoz, Biotechnology Laboratory, Instituto de Investigaciones Agropecuarias (INIA) -Centro Regional de Investigación La Platina, Santiago, Chile Zucchini yellow mosaic virus (ZYMV) was described in northern Italy (18). Soon thereafter, it was identified throughout the world in cucurbit-cultivated areas, including Mediterranean countries, China, Australia, Hawaii, and the continental United States (3,4,9,12,20,22).…”

Section: Isolation and Molecular Characterization Of A Chilean Isolatmentioning

confidence: 98%

Isolation and Molecular Characterization of a Chilean Isolate of Zucchini yellow mosaic virus

et al. 2001

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Zucchini yellow mosaic virus (ZYMV) was described in 1981 affecting squash, melon, and other cultivated cucurbits with severe stunting and yellowing symptoms. It was reported to be present in most countries where cucurbits are grown, and in Chile since 1995, from surveys using enzyme-linked immunosorbent assay (ELISA) but without further characterization. A potyvirus was isolated from ELISA-positive symptomatic plants. The results indicate that this virus is ZYMV based on symptoms on herbaceous indicators, immunospecific electron microscopy of the purified particle, and sequencing of 395 bases of the 3′ end of the coat protein gene. The virus was detected in melon, watermelon, and squash plants. In agreement with previous descriptions for ZYMV, the Chilean isolate is a flexuous filamentous particle 740 nm long with one main protein of approximately 36 kDa. Nucleotide sequence comparisons of the 3′ portion of the coat protein gene revealed a high similarity to the Connecticut and California strains.

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“…Detection of ZYMV was also possible using Ouchterlouny gel double-diffusion tests in a medium containing 0.8% agar, 1% sodium azide and 0.5% sodium dodecyl sulphate (SDS-ID) as described by Purcifull & Batchelor (1977). This method contributed to the rapid and practical detection of ZYMV in several countries Greber et al, 1987). SDS-ID is also useful to detect other mosaicinducing viruses in cucurbits (Purcifull et al, 1988) and is therefore very convenient for establishing diagnosis in a limited number of samples.…”

Section: Serological Techniquesmentioning

confidence: 99%

“…Using antisera against virions and SDS-immunodiffusion, no serological cross-reaction was observed between ZYMV and PRSV, WMVMorocco, bean yellow mosaic (BYMV), ZYFV, clover yellow vein (ClYVV), lettuce mosaic (LMV), and wisteria vein mosaic (WVMV) potyviruses (Lisa et al, 1981;Lisa & Lecoq, 1984) but some cross-reactions were consistently detected between ZYMV and WMV2 antisera Lisa et al, 1981;Davis, 1986;Greber et al, 1987;Somowiyarjo et al, 1989). This crossreactivity depends on the antiserum used for the tests.…”

Section: Serological Relationships With Other Virusesmentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

223

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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Zucchini Yellow Mosaic Virus in Three States of Australia.

Cited by 25 publications

References 9 publications

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

Zucchini yellow mosaic virus — incidence and sources of virus infection in field-grown cucumbers and pumpkins in the Spreewald, Germany

Isolation and Molecular Characterization of a Chilean Isolate of Zucchini yellow mosaic virus

Zucchini yellow mosaic virus

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