ZSCAN16-AS1 expedites hepatocellular carcinoma progression via modulating the miR-181c-5p/SPAG9 axis to activate the JNK pathway

Liu, Jianwen; Liu, Ruiqing; Liu, Yuyan; Li, Lupeng; Cao, Huicun; Liu, Jian; Cao, Guangshao

doi:10.1080/15384101.2021.1919828

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…Other lncRNAs also play roles in important biological pathways. For example, by regulating mRNA expression through miRNA, AC145207.5 participates in cellular immunity, MIR34AHG is an ERS-related lncRNA ( Chen et al, 2022a ), SPAG9 activates the JNK signaling pathway ( Liu et al, 2021a ), and nucleolar RNA host genes all play roles in cardiac-related diseases. These regulated pathways are very similar to the biological processes that are dysregulated in DCM.…”

Section: Discussionmentioning

confidence: 99%

Endoplasmic reticulum stress-related gene expression causes the progression of dilated cardiomyopathy by inducing apoptosis

Chen,

Yang,

et al. 2024

Front. Genet.

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Background: Previous studies have shown that endoplasmic reticulum stress (ERS) -induced apoptosis is involved in the pathogenesis of dilated cardiomyopathy (DCM). However, the molecular mechanism involved has not been fully characterized.Results: In total, eight genes were obtained at the intersection of 1,068 differentially expressed genes (DEGs) from differential expression analysis between DCM and healthy control (HC) samples, 320 module genes from weighted gene co-expression network analysis (WGCNA), and 2,009 endoplasmic reticulum stress (ERGs). These eight genes were found to be associated with immunity and angiogenesis. Four of these genes were related to apoptosis. The upregulation of MX1 may represent an autocompensatory response to DCM caused by a virus that inhibits viral RNA and DNA synthesis, while acting as an autoimmune antigen and inducing apoptosis. The upregulation of TESPA1 would lead to the dysfunction of calcium release from the endoplasmic reticulum. The upregulation of THBS4 would affect macrophage differentiation and apoptosis, consistent with inflammation and fibrosis of cardiomyocytes in DCM. The downregulation of MYH6 would lead to dysfunction of the sarcomere, further explaining cardiac remodeling in DCM. Moreover, the expression of genes affecting the immune micro-environment was significantly altered, including TGF-β family member. Analysis of the co-expression and competitive endogenous RNA (ceRNA) network identified XIST, which competitively binds seven target microRNAs (miRNAs) and regulates MX1 and THBS4 expression. Finally, bisphenol A and valproic acid were found to target MX1, MYH6, and THBS4.Conclusion: We have identified four ERS-related genes (MX1, MYH6, TESPA1, and THBS4) that are dysregulated in DCM and related to apoptosis. This finding should help deepen understanding of the role of endoplasmic reticulum stress-induced apoptosis in the development of DCM.

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Section: Discussionmentioning

confidence: 99%

Endoplasmic reticulum stress-related gene expression causes the progression of dilated cardiomyopathy by inducing apoptosis

Chen,

Yang,

et al. 2024

Front. Genet.

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“…28,29 ZSCAN16-AS1, a newly recognized lncRNA, is over-regulated in liver cancer and promotes the malignant properties of hepatocellular carcinoma. 13,14 It has been reported that it is also highly expressed in malignant melanoma. 15 However, no one has studied the specific function and biological mechanism of ZSCAN16-AS1 in melanoma.…”

Section: Dovepressmentioning

confidence: 99%

“…32 Similarly, ZSCAN16-AS1 has been demonstrated to expedite the malignant progression of hepatocellular carcinoma by modulating the miR-181c-5p/SPAG9/JNK and miR-451a/ATF2 pathways. 13,14 Here, we used bioinformatics software and the TCGA database to identify the ceRNA network of ZSCAN16-AS1 in melanoma, and found that ZSCAN16-AS1, miR-503-5p and ARL2 have potential ceRNA correlations. By using luciferase reporter and RNA pull-down assays, we proved that ARL2 is a direct target gene of miR-503-5p and that miR-503-5p and ZSCAN16-AS1 have a direct binding relationship in melanoma.…”

Section: Dovepressmentioning

confidence: 99%

“…11,12 LncRNA zinc finger and SCAN domain containing 16 antisense RNA 1 (ZSCAN16-AS1), a brand new lncRNA, is highly expressed in hepatocellular carcinoma and expedites hepatocellular carcinoma progression. 13,14 It was reported that ZSCAN16-AS1 is overexpressed in melanoma tissues, 15 but the function and molecular mechanism of ZSCAN16-AS1 in melanoma are not very clear.…”

Section: Introductionmentioning

confidence: 99%

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Long Non-Coding RNA ZSCAN16-AS1 Promotes the Malignant Progression of Melanoma Through Regulating the miR-503-5p/ARL2 Axis

Zhao¹,

Zhang²,

Wang³

et al. 2023

CCID

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Background: LncRNA zinc finger and SCAN domain containing 16 antisense RNA 1 (ZSCAN16-AS1), a newly identified lncRNA, has been proven to accelerate hepatocellular carcinoma progression. However, the function and molecular mechanism of ZSCAN16-AS1 in melanoma are still unknown. Methods: The level of ZSCAN16-AS1 in melanoma tissues was detected and reported in The Cancer Genome Atlas (TCGA) and GEO#GSE15605. CCK-8, Transwell and flow cytometry assays were used to explore the role of ZSCAN16-AS1 in melanoma cells. Luciferase reporter assays and RNA pull-down assays were used to verify the molecular mechanism of ZSCAN16-AS1. Results: Here, we found that ZSCAN16-AS1 expression was increased in melanoma. We confirmed that ZSCAN16-AS1 promotes the growth and metastasis of melanoma. ZSCAN16-AS1 exerts its pro-tumour role through sponging of miR-503-5p to liberate ADPribosylation factor-like protein 2 (ARL2) mRNA transcripts. Conclusion: These results demonstrated the role and molecular mechanism of ZSCAN16-AS1 in the occurrence and development of melanoma. Therefore, ZSCAN16-AS1 may be used as a specific biomarker in the diagnosis and treatment of melanoma patients.

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“…The SCAN domain, also known as the leucine rich region (LeR), is a conserved 84 residue motif; more than 71 domains have been identified in the human genome (15). However, although several studies have reported the association of ZSCAN family members with lipid metabolism, cell growth, and differentiation (16)(17)(18), the function of most members in this group has remained elusive.…”

Section: Introductionmentioning

confidence: 99%

Zinc finger and SCAN domain containing 1, ZSCAN1, is a novel stemness-related tumor suppressor and transcriptional repressor in breast cancer targeting TAZ

Chu

Li²,

He³

et al. 2023

Front. Oncol.

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IntroductionCancer stem cells (CSCs) targeted therapy holds the potential for improving cancer management; identification of stemness-related genes in CSCs is necessary for its development.MethodsThe Cancer Genome Atlas (TCGA) and the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) datasets were used for survival analysis. ZSCAN1 correlated genes was identified by Spearman correlation analysis. Breast cancer stem-like cells (BCSLCs) were isolated by sorting CD44+CD24- cells from suspension cultured breast cancer (BC) spheroids. The sphere-forming capacity and sphere- and tumor-initiating capacities were determined by sphere formation and limiting dilution assays. The relative gene expression was determined by qRT-PCR, western blot. Lentivirus system was used for gene manipulation. Nuclear run-on assay was employed to examine the levels of nascent mRNAs. DNA pull-down and Chromatin immunoprecipitation (ChIP) assays were used for determining the interaction between protein and target DNA fragments. Luciferase reporter assay was used for evaluating the activity of the promoter.Results and discussionZSCAN1 is aberrantly suppressed in BC, and this suppression indicates a bad prognosis. Ectopic expression of ZSCAN1 inhibited the proliferation, clonogenicity, and tumorigenicity of BC cells. ZSCAN1-overexpressing BCSLCs exhibited weakened stemness properties. Normal human mammary epithelial (HMLE) cells with ZSCAN1 depletion exhibited enhanced stemness properties. Mechanistic studies showed that ZSCAN1 directly binds to -951 ~ -925bp region of WWTR1 (encodes TAZ) promoter, inhibits WWTR1 transcription, thereby inhibiting the stemness of BCSCs. Our work thus revealed ZSCAN1 as a novel stemness-related tumor suppressor and transcriptional repressor in BC.

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ZSCAN16-AS1 expedites hepatocellular carcinoma progression via modulating the miR-181c-5p/SPAG9 axis to activate the JNK pathway

Cited by 9 publications

References 39 publications

Endoplasmic reticulum stress-related gene expression causes the progression of dilated cardiomyopathy by inducing apoptosis

Endoplasmic reticulum stress-related gene expression causes the progression of dilated cardiomyopathy by inducing apoptosis

Long Non-Coding RNA ZSCAN16-AS1 Promotes the Malignant Progression of Melanoma Through Regulating the miR-503-5p/ARL2 Axis

Zinc finger and SCAN domain containing 1, ZSCAN1, is a novel stemness-related tumor suppressor and transcriptional repressor in breast cancer targeting TAZ

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