ZRF1 Chromatin Regulators Have Polycomb Silencing and Independent Roles in Development

Feng, Jing; Chen, Donghong; Berr, Alexandre; Shen, Wen‐Hui

doi:10.1104/pp.16.00193

Cited by 17 publications

(37 citation statements)

References 55 publications

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“…In Arabidopsis , two ZRF1 homologs, AtZRF1a and AtZRF1b, showed redundant functions. AtZRF1b can bind ubiquitin in vitro and pull-down H2Aub1 and H2A from plant protein extracts (Feng et al, 2016), which is in agreement with the human ZRF1 acting as a H2Aub1 reader. The AtZRF1a/b genes display broad expression pattern, but with higher levels in the dividing cell-enriched tissues, e.g.…”

Section: Introductionsupporting

confidence: 59%

“…The atzrf1a-1;atzrf1b-1 and atzrf1a-2;atzrf1b-1 double mutants (Feng et al, 2016) and some marker lines CYCB1;1:Dbox-GUS (Colon-Carmona et al, 1999), WOX5 :: erGFP (Blilou et al, 2005), SCR::SCR-YFP (Heidstra et al, 2004), CO2 :: H2B - YFP (Heidstra et al, 2004), DR5rev :: GFP (Friml et al, 2003) have been described previously. The Haseloff enhancer trap GFP lines J1092 (N9147) and J2341 (N9118) were obtained from the Arabidopsis Biological Resource Center () or the European Arabidopsis Stock Centre ().…”

Section: Methodsmentioning

confidence: 99%

“…meristem, floral bud, and developing embryo. Loss-of-function of AtZRF1a/b causes pleiotropic abnormalities including delayed seed germination, plant dwarfism, formation of multiple ectopic meristems, and defects in flower development and gametophyte transmission as well as embryogenesis (Feng et al, 2016; Guzman-Lopez et al, 2016). The atzrf1a;atzrf1b mutant displays severely disrupted root developmental phenotype; yet, the underlying mechanism is far from clear.…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis ZUOTIN RELATED FACTOR1 Proteins Are Required for Proper Embryonic and Post-Embryonic Root Development

et al. 2019

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The H2A/UBIQUITIN-binding proteins AtZRF1a/b have been reported as key regulators involved in multiple processes of Arabidopsis plant growth and development. Yet, the cellular and molecular mechanisms underlying the mutant phenotype remain largely elusive. Here we show that loss-of-function of AtZRF1a/b causes defective root elongation and deformed root apical meristem organization in seedlings. The premature termination of the primary root in the atzrf1a;atzrf1b double mutant is associated with an advanced onset of endoreduplication and subsequent consumption of reservoir stem cells. Cytological analyses using cell type-specific markers and florescent dyes indicate that AtZRF1a/b are involved in maintenance of proper cell layer organization, determinacy of cell identity, and establishment of auxin gradient and maximum at the root tip. During embryogenesis AtZRF1a/b act dominantly in regulating the maintenance of ground tissue initial cells and production of lateral root cap. Lastly, quantitative real-time polymerase chain reaction analysis shows mis-expression of some key genes involved in regulating cell patterning, cell proliferation and/or hormone pathways. Our results provide important insight into AtZRF1a/b function in cell fate determinacy and in establishment and maintenance of proper stem cell reservoir during embryonic and post-embryonic root development.

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Section: Introductionsupporting

confidence: 59%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Arabidopsis ZUOTIN RELATED FACTOR1 Proteins Are Required for Proper Embryonic and Post-Embryonic Root Development

et al. 2019

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“…Additional repressors of seed maturation genes have been found in vegetative organs and germinating seeds [162]. These include the polycomb EMF2-PRC2 complex combined with the SDG8 methyltransferase, which are required to maintain the H3K27me3 repressive mark in seedlings [163]; two ZRF proteins that contribute to PCR1-mediated repression by binding to monoubiquitinated H2As and H3K27me3 [164]; the SUVH5 methyltransferase mediating repressive dimethylation of H3K9 [165]; LDL1/2 demethylases, which potentially remove activating histone modifications (H3K4me2/3) from seed dormancy genes [166,167]. Finally, deacetylation of H2B by the HD2B deacetylase is associated with reduced dormancy and increased GA levels in imbibed seeds.…”

Section: Germination Control By the Epigenomementioning

confidence: 99%

An Updated Overview on the Regulation of Seed Germination

Carrera-Castaño

Calleja-Cabrera

Pernas

et al. 2020

Plants

131

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The ability of a seed to germinate and establish a plant at the right time of year is of vital importance from an ecological and economical point of view. Due to the fragility of these early growth stages, their swiftness and robustness will impact later developmental stages and crop yield. These traits are modulated by a continuous interaction between the genetic makeup of the plant and the environment from seed production to germination stages. In this review, we have summarized the established knowledge on the control of seed germination from a molecular and a genetic perspective. This serves as a “backbone” to integrate the latest developments in the field. These include the link of germination to events occurring in the mother plant influenced by the environment, the impact of changes in the chromatin landscape, the discovery of new players and new insights related to well-known master regulators. Finally, results from recent studies on hormone transport, signaling, and biophysical and mechanical tissue properties are underscoring the relevance of tissue-specific regulation and the interplay of signals in this crucial developmental process.

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“…For instance, mutants affected in ATRX7 (H3K4 methyltransferase), EFS/SDG8 (H3K36 di-and trimethylation), EBS (an H3K4me2/3 "reader" interacting with HDA6) or HUB1 and HUB2 (H2B monoubiquitination) display lower ABI3 expression and dormancy, whereas those affected in H3K9 methylation (kyp/suvh4) or H3K4 demethylation (ldl1 and ldl2) display the opposite phenotype (Liu et al, 2007b;Bassel et al, 2011;Zheng et al, 2012;Zhao et al, 2015;Narro-Diego et al, 2017). Moreover, although PRC1/2 are not required for proper embryo development, mutations affecting PRC1 or PRC2 lead to a failure in both, the repression of LAFL expression and directly switch from embryo to seedling development (Chanvivattana et al, 2004;Makarevich et al, 2006;Aichinger et al, 2009;Bratzel et al, 2010;Chen et al, 2010;Berger et al, 2011;Bouyer et al, 2011;Muller et al, 2012;Tang et al, 2012a;Zhang et al, 2012;Deng et al, 2013;Yang et al, 2013;Molitor et al, 2014;Ikeuchi et al, 2015;Xiao and Wagner, 2015;Feng et al, 2016;Trindade et al, 2017;Xiao et al, 2017;Lee and Seo, 2018). Similar accumulation of seed proteins in vegetative tissues is also observed when the RETINOBLASTOMA-RELATED1 (RBR1) that is required to maintain the PRC2-dependent repression of LEC2 and ABI3 is affected (Gutzat et al, 2011;Kuwabara and Gruissem, 2014) and in mutant of the SWI2/SNF2 chromatin remodelling ATPase BRAHMA (BRM) (Tang et al, 2008), although BRM has been shown to controls the transition from juvenile to adult vegetative phase antagonistically to PRC2 (Xu et al, 2016).…”

Section: Chromatin-based Regulationsmentioning

confidence: 99%

Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development

et al. 2018

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The LAFL (i.e. LEC1, ABI3, FUS3, and LEC2) master transcriptional regulators interact to form different complexes that induce embryo development and maturation, and inhibit seed germination and vegetative growth in Arabidopsis. Orthologous genes involved in similar regulatory processes have been described in various angiosperms including important crop species. Consistent with a prominent role of the LAFL regulators in triggering and maintaining embryonic cell fate, their expression appears finely tuned in different tissues during seed development and tightly repressed in vegetative tissues by a surprisingly high number of genetic and epigenetic factors. Partial functional redundancies and intricate feedback regulations of the LAFL have hampered the elucidation of the underpinning molecular mechanisms. Nevertheless, genetic, genomic, cellular, molecular, and biochemical analyses implemented during the last years have greatly improved our knowledge of the LALF network. Here we summarize and discuss recent progress, together with current issues required to gain a comprehensive insight into the network, including the emerging function of LEC1 and possibly LEC2 as pioneer transcription factors.

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ZRF1 Chromatin Regulators Have Polycomb Silencing and Independent Roles in Development

Cited by 17 publications

References 55 publications

Arabidopsis ZUOTIN RELATED FACTOR1 Proteins Are Required for Proper Embryonic and Post-Embryonic Root Development

Arabidopsis ZUOTIN RELATED FACTOR1 Proteins Are Required for Proper Embryonic and Post-Embryonic Root Development

An Updated Overview on the Regulation of Seed Germination

Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development

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