“…The protein concentration was determined using the BCA method (Thermo Fisher Scientific, USA). Western blotting was performed following a standard protocol, as described previously [24]. The following primary antibodies were used: anti-TRPA1 (110 kDa, 1 : 1000, rabbit polyclonal, Novus Biologicals, USA), anti-Postn (93 kDa, 1 : 1000, rabbit polyclonal, Novus Biologicals, USA), anti-NFATc3 (115 kDa, 1 : 1000, rabbit polyclonal, Abcam, USA), anti-DYRK1A (86 kDa, 1 : 1000, rabbit polyclonal, Abcam, USA), anti- β -actin (43 kDa, 1 : 1,000, rabbit polyclonal, Santa Cruz Biotechnology Inc., USA), anti-GAPDH (36 kDa, 1 : 1000, rabbit monoclonal, Abcam, USA), and anti-Histone H3 (15 kDa, 1 : 1000, rabbit monoclonal, Abcam, USA).…”