Zoysia japonica MYC type transcription factor ZjICE1 regulates cold tolerance in transgenic Arabidopsis

Zuo, Zhi-Fang; Kang, Hong‐Gyu; Park, Mi-Young; Jeong, Hana; Sun, Hui; Song, Pill‐Soon; Lee, Hyo-Yeon

doi:10.1016/j.plantsci.2019.110254

Cited by 44 publications

(31 citation statements)

References 69 publications

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“…In addition, RD29A /COR78 is a stress-related target gene whose expression was higher in the transgenic plants than that of the WT at room temperature ( Figure 11 I (0 h)). However, no consistent up-regulated RD29A /COR78 gene expression, in contrast to other transgenic Arabi d opsis with VaICE1 , VaICE2 , ZmmICE1 , HbICE1 , ZjICE1, or SmICE1 [ 41 , 42 , 43 , 44 , 45 ], was obtained in transgenic plants under cold/freezing stress ( Figure 11 I (3 h)). Therefore, we propose that CBF2/DREB1 differentially regulates the gene expression of CBF1/DREB1B and CBF3/DREB1A , thereby contributing to their different downstream target gene ( COR15A , COR47 , KIN1 , and RD29A /COR78 ) expression.…”

Section: Discussion/conclusionmentioning

confidence: 86%

“…In addition, overexpressed AtICE2 causes the increased expression of the downstream CBF1 gene, indicating that AtICE2 is involved in cold tolerance through controlling CBF gene expression under cold stress [ 35 ]. Most recently, AtICE homologs have subsequently been found in several other plant species, and these ICE-like proteins, when overexpressed in transgenic plants, were revealed to increase stress tolerance [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ]. For example, dehydrin-like gene ( SiDhn2 ) and stearoyl-acyl carrier protein desaturase ( SikSACPD ), related to cold acclimation in snow lotus ( S. involucrata ), have been cloned, and transgenic plants showed greater resistance to freezing and drought stress than the wild-type plants [ 46 , 47 ].…”

Section: Introductionmentioning

confidence: 99%

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Saussurea involucrata (Snow Lotus) ICE1 and ICE2 Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic Arabidopsis

Lin

Hsu³

et al. 2021

IJMS

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As with other environmental stresses, cold stress limits plant growth, geographical distribution, and agricultural productivity. CBF/DREB (CRT-binding factors/DRE-binding proteins) regulate tolerance to cold/freezing stress across plant species. ICE (inducer of CBF expression) is regarded as the upstream inducer of CBF expression and plays a crucial role as a main regulator of cold acclimation. Snow lotus (Saussurea involucrata) is a well-known traditional Chinese herb. This herb is known to have greater tolerance to cold/freezing stress compared to other plants. According to transcriptome datasets, two putative ICE homologous genes, SiICE1 and SiICE2, were identified in snow lotus. The predicted SiICE1 cDNA contains an ORF of 1506 bp, encoding a protein of 501 amino acids, whereas SiICE2 cDNA has an ORF of 1482 bp, coding for a protein of 493 amino acids. Sequence alignment and structure analysis show SiICE1 and SiICE2 possess a S-rich motif at the N-terminal region, while the conserved ZIP-bHLH domain and ACT domain are at the C-terminus. Both SiICE1 and SiICE2 transcripts were cold-inducible. Subcellular localization and yeast one-hybrid assays revealed that SiICE1 and SiICE2 are transcriptional regulators. Overexpression of SiICE1 (35S::SiICE1) and SiICE2 (35S::SiICE2) in transgenic Arabidopsis increased the cold tolerance. In addition, the expression patterns of downstream stress-related genes, CBF1, CBF2, CBF3, COR15A, COR47, and KIN1, were up-regulated when compared to the wild type. These results thus provide evidence that SiICE1 and SiICE2 function in cold acclimation and this cold/freezing tolerance may be regulated through a CBF-controlling pathway.

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Section: Discussion/conclusionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Saussurea involucrata (Snow Lotus) ICE1 and ICE2 Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic Arabidopsis

Lin

Hsu³

et al. 2021

IJMS

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“…The overexpression of BcICE1 in tobacco can positively regulate the expression of stress-related genes such as CBFs (C-repeat binding factor) and enhance the antioxidant activity and osmotic ability of plants (Zhang et al, 2018). Zuo et al (2019) showed that the transgenic Arabidopsis with overexpressed Zoysia japonica ZjICE1 showed an enhanced tolerance to cold stress with an increase in SOD, POD, as well as higher free proline content and decreased MDA content. They also upregulated the transcript abundance of cold-responsive genes (CBF1, CBF2, CBF3, COR47A, KIN1, and RD29A).…”

Section: Bhlhs Adjust Plants To Low Temperature Stressmentioning

confidence: 99%

“…They also upregulated the transcript abundance of cold-responsive genes (CBF1, CBF2, CBF3, COR47A, KIN1, and RD29A). ZjICE2 from Zoysia japonica enhanced the tolerance of transgenic plants to cold stress by activating DREB/CBF regulators and enhancing reactive oxygen species scavenging (Zuo et al, 2019). In addition to ICE proteins, some bHLH proteins are also responsible for plant resistance to low temperatures.…”

Section: Bhlhs Adjust Plants To Low Temperature Stressmentioning

confidence: 99%

Regulatory Mechanisms of bHLH Transcription Factors in Plant Adaptive Responses to Various Abiotic Stresses

Qian

Zhang

et al. 2021

Front. Plant Sci.

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Basic helix-loop-helix proteins (bHLHs) comprise one of the largest families of transcription factors in plants. They have been shown to be involved in responses to various abiotic stresses, such as drought, salinity, chilling, heavy metal toxicity, iron deficiency, and osmotic damages. By specifically binding to cis-elements in the promoter region of stress related genes, bHLHs can regulate their transcriptional expression, thereby regulating the plant’s adaptive responses. This review focuses on the structural characteristics of bHLHs, the regulatory mechanism of how bHLHs are involved transcriptional activation, and the mechanism of how bHLHs regulate the transcription of target genes under various stresses. Finally, as increasing research demonstrates that flavonoids are usually induced under fluctuating environments, the latest research progress and future research prospects are described on the mechanisms of how flavonoid biosynthesis is regulated by bHLHs in the regulation of the plant’s responses to abiotic stresses.

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“…Anthocyanins may scavenge ROS as an efficient antioxidant by neutralizing radicals with their hydroxyl groups to preserve normal cellular redox homeostasis; hence, plants often generate anthocyanins to reduce oxidative damage caused by low-temperature stress [ 20 , 21 , 22 , 23 , 24 , 25 ]. Low-temperature responsive networks in zoysiagrass have been investigated through physiological, biochemical, molecular, genetic and omic approaches [ 7 , 15 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. However, no studies have attempted to associate anthocyanin accumulation with cold tolerance in zoysiagrass.…”

Section: Introductionmentioning

confidence: 99%

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

Jin

Jiang

Yang

et al. 2022

Plants

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Zoysia japonica is a warm-season turfgrass that is extensively used in landscaping, sports fields, and golf courses worldwide. Uncovering the low-temperature response mechanism of Z. japonica can help to accelerate the development of new cold-tolerant cultivars, which could be used to prolong the ornamental and usage duration of turf. A novel Z. japonica biotype, YueNong-9 (YN-9), was collected from northeastern China for this study. Phenotypic measurements, cold-tolerance investigation, and whole-transcriptome surveys were performed on YN-9 and LanYin-3 (LY-3), the most popular Z. japonica cultivar in Southern China. The results indicated the following: YN-9 has longer second and third leaves than LY-3; when exposed to the natural low temperature during winter in Guangzhou, YN-9 accumulated 4.74 times more anthocyanin than LY-3; after cold acclimation and freezing treatment, 83.25 ± 9.55% of YN-9 survived while all LY-3 leaves died, and the dark green color index (DGCI) value of YN-9 was 1.78 times that of LY-3; in YN-9, there was a unique up-regulation of Phenylalanine ammonia-lyase (PAL), Homeobox-leucine Zipper IV (HD-ZIP), and ATP-Binding Cassette transporter B8 (ABCB8) expressions, as well as a unique down-regulation of zinc-regulated transporters and iron-regulated transporter-like proteins (ZIPs) expression, which may promote anthocyanin biosynthesis, transport, and accumulation. In conclusion, YN-9 exhibited enhanced cold tolerance and is thus an excellent candidate for breeding cold-tolerant Z. japonica variety, and its unique low-temperature-induced anthocyanin accumulation and gene responses provide ideas and candidate genes for the study of low-temperature tolerance mechanisms and genetic engineering breeding.

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Zoysia japonica MYC type transcription factor ZjICE1 regulates cold tolerance in transgenic Arabidopsis

Cited by 44 publications

References 69 publications

Saussurea involucrata (Snow Lotus) ICE1 and ICE2 Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic Arabidopsis

Saussurea involucrata (Snow Lotus) ICE1 and ICE2 Orthologues Involved in Regulating Cold Stress Tolerance in Transgenic Arabidopsis

Regulatory Mechanisms of bHLH Transcription Factors in Plant Adaptive Responses to Various Abiotic Stresses

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

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