Zoothamnium duplicatum infestation of cultured horseshoe crabs (Limulus polyphemus)

Shinn, Andrew P.; Mühlhölzl, Alexander P.; Coates, Christopher J.; Metochis, Christoforos; Freeman, Mark

doi:10.1016/j.jip.2014.12.002

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…Although the development of horseshoe crab aquaculture is still in its infancy, various laboratory studies have reported culture conditions for juvenile horseshoe crab species in experimental investigations (Carmichael & Brush 2012;Shinn, M€ uhlh€ olzl, Coates, Metochis & Freeman 2015). Some of these studies have demonstrated that protein-rich diets of brine shrimp, clams, mussels and polychaetes can enhance the growth performance of juveniles (Carmichael, Gaines, Sheller, Tong, Clapp & Valiela 2009;Schreibman & Zarnoch 2009;Hu, Wang, Cheung & Shin 2012); however, Kwan, Chan, Cheung and Shin (2014) revealed that the haemolymph quality in juvenile Chinese horseshoe crabs under laboratory culture was significantly reduced over a 12-week experimental period even under high-protein diet treatments.…”

Section: Introductionmentioning

confidence: 99%

Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs,Tachypleus tridentatus(Xiphosura)

et al. 2016

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Five treatments (short‐neck clam Ruditapes philippinarum and four formulated diets with the green microalgae Dunaliella tertiolecta or the golden‐brown microalgae Isochrysis galbana in the ratio (dw/dw) of 5% or 10% to that of clam meat) were used to investigate the growth performance and haemolymph quality of juvenile Chinese horseshoe crabs Tachypleus tridentatus under laboratory culture. At the end of the 12‐week study, various growth and moulting indicators as well as haemolymph biochemical parameters did not show any significant differences among the treatments. Significantly higher percentages of haemolymph amoebocyte viability and granular‐spherical amoebocyte state were noted in both 5% and 10% I. galbana‐supplemented diets compared to that of clam meat. However, the percentages of these two haemolymph parameters in juveniles fed with D. tertiolecta decreased significantly at the end of the experiment. Such differences were attributed to the presence of polyunsaturated fatty acid (PUFA), notably eicosapentaenoic acid (EPA), in I. galbana that was absent in D. tertiolecta and clam meat. Like many marine invertebrates, juvenile horseshoe crabs do not naturally obtain sufficient PUFAs from their diets to meet developmental and physiological needs; hence, supplementing microalgae with high levels of EPA in a protein‐rich diet boosts immune competence and the health status of juvenile horseshoe crabs while under culture.

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Section: Introductionmentioning

confidence: 99%

Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs,Tachypleus tridentatus(Xiphosura)

et al. 2016

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“…The observations on the morphology of ectoparasites of mud crabs (S. serrata) and white shrimps (L. vannamei) showed slight differences when compared to those that have been recorded by Ma and Overstreet (2006), Jie et al (2006), Chan et al (2009), Ihwan et al (2014), Xinlu et al (2014) and Shinn et al (2015).…”

Section: Resultsmentioning

confidence: 67%

Ectoparasites of mangrove crabs (Scylla serrata) and white shrimps (Litopenaeus vannamei) from Gresik, Indonesia

Puspitasari¹,

Sari²,

Kismiyati³

2020

Journ. of Veterin. Parasitol.

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The ectoparasites that infest two hosts, mangrove crabs (Scylla serrata) and white shrimps (Litopenaeus vannamei) of the subphylum crustacea were studied. The study revealed that Octolasmis sp., Zoothamnium sp. and Epistylis sp. were found to infest mangrove crabs, while Zoothamnium sp. and Epistylis sp. were found parasitizing the white shrimp (Litopenaeus vannamei). The most common organ attacked by the ectoparasites was the gills. Stagnant water with limited or no flow of water in the ponds was an important factor that supported the infestation of parasites.

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“…They are known bacterial grazers, hence generally influence bacterial epibiosis. However, the ciliate genus Zoothamnium, which was detected in high abundances on FV is also known as a marine pathogen (Shinn et al, 2015) and may contribute in part to the highly fouled surface observed. Significantly abundant ascomycete genus on FV was the yeast Candida, which might contribute to microfouling and consume algal exudates.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and Microbiomics Insights into the Differential Surface Fouling of Brown Algae

Oppong-Danquah

Blümel

Taşdemir

2023

Preprint

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Marine macroalgae (seaweeds) are key components of marine ecosystems with vital roles in coastal habitats. As they release dissolved organic matter and other molecules, seaweeds are under strong settlement pressure by micro- and macro-epibionts. Uncontrolled epibiosis causes surface fouling with detrimental effects on the health and well-being of the organism. Seaweeds control surface epibionts directly by releasing antifouling and antimicrobial metabolites onto their surfaces, and indirectly by recruiting beneficial microorganisms that produce antimicrobial/antifouling metabolites. Three species of the brown algal genusFucus,F. vesiculosus(FV),F. serratus(FS) andF. distichussubsp.evanescens(FE) form theFucusbelt habitat in the Kiel Fjord, Germany. They often co-occur in the same spot but their blades are fouled differently; we observed FE to be the least fouled, and FV to be the most fouled species. This study was designed to investigate the potential factors underlying different fouling intensities on the surfaces of the three co-occurringFucusspp. Their surface metabolomes were analysed by comparative untargeted UPLC-MS/MS based metabolomics to identify marker metabolites influencing the surface fouling. The epiphytic microbial communities of theFucusspp. were also comparatively characterized by high-throughput amplicon sequencing to identify the differences in the surface microbiome of the algae. By employing these omics methods, integrated with multivariate analyses, we identified discriminant metabolites and microbial taxa associated with FE surface, including antimicrobial polar lipids, the fungal generaMucor,Alternaria, and bacterial genusYoonia-Loktanella. These taxa have been previously reported to produce antimicrobial and antifouling compounds, suggesting their potential involvement in the fouling resistance (least fouled) observed on the FE surface relative to the co-occurring algae FS and FV. These findings shed light on the surface metabolome and microbiome ofFucusspp. and their influence in different fouling intensities and also have implications for the conservation of coastal habitats.

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Zoothamnium duplicatum infestation of cultured horseshoe crabs (Limulus polyphemus)

Cited by 13 publications

References 25 publications

Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs,Tachypleus tridentatus(Xiphosura)

Marine microalgae as dietary supplements in the culture of juvenile Chinese horseshoe crabs,Tachypleus tridentatus(Xiphosura)

Ectoparasites of mangrove crabs (Scylla serrata) and white shrimps (Litopenaeus vannamei) from Gresik, Indonesia

Metabolomics and Microbiomics Insights into the Differential Surface Fouling of Brown Algae

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