Zoonotic transmission of reassortant porcine G4P[6] rotaviruses in Hungarian pediatric patients identified sporadically over a 15year period

Papp, Hajnalka; Borzák, Réka; Farkas, Szilvia L.; Kisfali, Péter; Lengyel, György; Molnár, Péter; Melegh, Béla; Matthijnssens, Jelle; Jakab, Ferenc; Martella, Vito; Bányai, Krisztiàn

doi:10.1016/j.meegid.2013.06.013

Cited by 64 publications

(59 citation statements)

References 49 publications

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“…In the present study, ancient strain RV277 from 1977 also possessed an I1 genotype for VP6. Furthermore, the I1 genotype was also recently detected in pig- Theuns et al like human strains from Hungary and the Democratic Republic of Congo (40,41). Several amino acid changes were observed at the apical site of the VP6 trimer (Fig.…”

Section: Discussionmentioning

confidence: 58%

Complete Genome Characterization of Recent and Ancient Belgian Pig Group A Rotaviruses and Assessment of Their Evolutionary Relationship with Human Rotaviruses

Theuns

Heylen

Zeller

et al. 2015

J Virol

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107

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Group) Belgian pig RVA strains and of all completely characterized pig RVAs from around the globe. In contrast to the large diversity of genotypes found for the outer capsid proteins VP4 and VP7, a relatively conserved genotype constellation (I5-R1-C1-M1-A8-N1-T7-E1-H1) was found for the other 9 genes in most pig RVA strains. VP1, VP2, VP3, NSP2, NSP4, and NSP5 genes of porcine RVAs belonged to genotype 1, which is shared with human Wa-like RVAs. However, for most of these gene segments, pig strains clustered distantly from human Wa-like RVAs, indicating that viruses from both species have entered different evolutionary paths. However, VP1, VP2, and NSP3 genes of some archival human strains were moderately related to pig strains. Phylogenetic analysis of the VP6, NSP1, and NSP3 genes, as well as amino acid analysis of the antigenic regions of VP7, further confirmed this evolutionary segregation. The present results also indicate that the species barrier is less strict for pig P[6] strains but that chances for successful spread of these strains in the human population are hampered by the better adaptation of pig RVAs to pig enterocytes. However, future surveillance of pig and human RVA strains is warranted. IMPORTANCERotaviruses are an important cause of diarrhea in many species, including pigs and humans. Our understanding of the evolutionary relationship between rotaviruses from both species is limited by the lack of genomic data on pig strains. In this study, recent and ancient Belgian pig rotavirus isolates were sequenced, and their evolutionary relationship with human Wa-like strains was investigated. Our data show that Wa-like human and pig strains have entered different evolutionary paths. Our data indicate that pig P[6] strains form the most considerable risk for interspecies transmission to humans. However, efficient spread of pig strains in the human population is most likely hampered by the adaptation of some crucial viral proteins to the cellular machinery of pig enterocytes. These data allow a better understanding of the risk for direct interspecies transmission events and the emergence of pig rotaviruses or pig-human reassortants in the human population. E nteric diseases in pigs are mostly encountered during two critical time points: the suckling period and after weaning. Several pathogens are frequently involved in the pathogenesis of piglet diarrhea, including rotavirus, porcine epidemic diarrhea virus, transmissible gastroenteritis virus, Escherichia coli, Clostridium perfringens, Salmonella spp., Brachyspira spp., and Isospora suis. Furthermore, coinfections between different rotaviruses and other pathogens can be found frequently in diarrheic pigs (1).Rotaviruses are a major cause of diarrhea in many species, including pigs and humans. Five official rotavirus species (A to E) and 3 tentative species (F to H) have been established based on nucleotide similarities of the VP6-encoding genes (2). Species A, B, and C are frequently isolated from feces of diarrheic and nondiarrheic pigs, wh...

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Section: Discussionmentioning

confidence: 58%

Complete Genome Characterization of Recent and Ancient Belgian Pig Group A Rotaviruses and Assessment of Their Evolutionary Relationship with Human Rotaviruses

Theuns

Heylen

Zeller

et al. 2015

J Virol

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107

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“…Although unusual in humans, a recent study describing G4P[6] strains in Hungarian children, reported three strains with the exact same genotype constellation as KisB332 [43]. The genotype constellation I1/I5-R1-C1-M1-A1/A8-N1-T1/T7-E1-H1 is typically found in pigs with a variety of different G- and P-genotypes [9], [44]–[46].…”

Section: Resultsmentioning

confidence: 99%

Rotavirus Surveillance in Kisangani, the Democratic Republic of the Congo, Reveals a High Number of Unusual Genotypes and Gene Segments of Animal Origin in Non-Vaccinated Symptomatic Children

et al. 2014

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Group A rotavirus (RVA) infections form a major public health problem, especially in low-income countries like the Democratic Republic of the Congo (COD). However, limited data on RVA diversity is available from sub-Saharan Africa in general and the COD in particular. Therefore, the first aim of this study was to determine the genetic diversity of 99 RVAs detected during 2007–2010 in Kisangani, COD. The predominant G-type was G1 (39%) and the most predominant P-type was P[6] (53%). A total of eight different G/P-combinations were found: G1P[8] (28%), G8P[6] (26%), G2P[4] (14%), G12P[6] (13%), G1P[6] (11%), G9P[8] (4%), G4P[6] (2%) and G8P[4] (1%). The second aim of this study was to gain insight into the diversity of P[6] RVA strains in the COD. Therefore, we selected five P[6] RVA strains in combination with the G1, G4, G8 (2x) or G12 genotype for complete genome analysis. Complete genome analysis showed that the genetic background of the G1P[6] and G12P[6] strains was entirely composed of genotype 1 (Wa-like), while the segments of the two G8P[6] strains were identified as genotype 2 (DS-1-like). Interestingly, all four strains possessed a NSP4 gene of animal origin. The analyzed G4P[6] RVA strain was found to possess the unusual G4-P[6]-I1-R1-C1-M1-A1-N1-T7-E1-H1 constellation. Although the majority of its genes (if not all), were presumably of porcine origin, this strain was able to cause gastro-enteritis in humans. The high prevalence of unusual RVA strains in the COD highlights the need for continued surveillance of RVA diversity in the COD. These results also underline the importance of complete genetic characterization of RVA strains and indicate that reassortments and interspecies transmission among human and animal RVAs strains occur regularly. Based on these data, RVA vaccines will be challenged with a wide variety of different RVA strain types in the COD.

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“…The occurrence of G9P [8] and G12P [8] strains was first recorded in 1998 and 2005, respectively, in our country (Bányai et al, 2004b(Bányai et al, , 2007. Unusual strains included the rare reassortants of common human G and P type specificities (e.g., G1P[4], G2P [8], G3P[4], and G9P[6]), whereas sporadic, rare strains included ovine-like P [14] strains, feline-human-bovine reassortant P[9] strains, and putative porcine origin G4P[6] strains (Bányai et al, 2009a(Bányai et al, , 2009b(Bányai et al, , 2010Papp et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Large-scale whole genome sequencing identifies country-wide spread of an emerging G9P[8] rotavirus strain in Hungary, 2012

Dóró

Mihalov-Kovács

Marton

et al. 2014

Infection, Genetics and Evolution

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Zoonotic transmission of reassortant porcine G4P[6] rotaviruses in Hungarian pediatric patients identified sporadically over a 15year period

Cited by 64 publications

References 49 publications

Complete Genome Characterization of Recent and Ancient Belgian Pig Group A Rotaviruses and Assessment of Their Evolutionary Relationship with Human Rotaviruses

Complete Genome Characterization of Recent and Ancient Belgian Pig Group A Rotaviruses and Assessment of Their Evolutionary Relationship with Human Rotaviruses

Rotavirus Surveillance in Kisangani, the Democratic Republic of the Congo, Reveals a High Number of Unusual Genotypes and Gene Segments of Animal Origin in Non-Vaccinated Symptomatic Children

Large-scale whole genome sequencing identifies country-wide spread of an emerging G9P[8] rotavirus strain in Hungary, 2012

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