Zoonotic potential of Helicobacter spp.

Mladenova-Hristova, Irena; Grekova, Olga; Patel, Ami

doi:10.1016/j.jmii.2016.11.003

Cited by 60 publications

(59 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Helicobacter is considered emerging bacteria with major public implications [ 27 , 28 ]; however, its epidemiology is not fully understood [ 29 ]. In the present study, we evaluated whether cows of the local breed represent a reservoir for H. pylori .…”

Section: Discussionmentioning

confidence: 99%

First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows

2018

View full text Add to dashboard Cite

Aim:The present study was conducted to detect and identify Helicobacter pylori within local cow breeds in the central region of Algeria.Materials and Methods:Two hundred (n=200) cows from three provinces of the central region of Algeria were studied, between January 2016 and September 2017. Each cow was subject to stool, milk, and blood sampling. Milk and fecal samples were used to detect and identify H. pylori using bacteriology culture method. Blood and milk samples were used to detect H. pylori immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay. Polymerase chain reaction was used to confirm the abundance of H. pylori in milk by detecting glmM gene.Results:Out of 200 sera and 200 milk samples, 12% (24) and 4% (8/200) were positive for the H. pylori IgG antibody. glmM gene was detected in the milk of 13% of cows and was confirmed in all cows presenting IgG in milk.Conclusion:From the present study, we concluded that the glmM gene is an important marker for detecting H. pylori in milk. Moreover, Algerian local-breed cows are a source of H. pylori and could be responsible for serious zoonosis.

show abstract

Section: Discussionmentioning

confidence: 99%

First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows

2018

View full text Add to dashboard Cite

show abstract

“…Furthermore, in the studies of Papiez et al[ 85 ] and Dore et al[ 65 ], H. pylori prevalence was higher in shepherds with direct animal contact than in controls without contact with sheep. Considering the 100% positive 13C-urea breath test in sheep, it may be reasonable to assume that these animal species may act as reservoirs and spreaders of H. pylori [ 85 , 86 ]. However, further epidemiological and experimental studies are needed to corroborate these few data.…”

Section: Evidence Supporting the Role Of Foods In Transmission Of mentioning

confidence: 99%

Helicobacter pylori: A foodborne pathogen?

Quaglia

Dambrosio

2018

WJG

View full text Add to dashboard Cite

Helicobacter pylori (H. pylori) is an organism that is widespread in the human population and is sometimes responsible for some of the most common chronic clinical disorders of the upper gastrointestinal tract in humans, such as chronic-active gastritis, duodenal and gastric ulcer disease, low-grade B-cell mucosa associated lymphoid tissue lymphoma of the stomach, and gastric adenocarcinoma, which is the third leading cause of cancer death worldwide. The routes of infection have not yet been firmly established, and different routes of transmission have been suggested, although the most commonly accepted hypothesis is that infection takes place through the faecal-oral route and that contaminated water and foods might play an important role in transmission of the microorganism to humans. Furthermore, several authors have considered H. pylori to be a foodborne pathogen because of some of its microbiological and epidemiological characteristics. H. pylori has been detected in drinking water, seawater, vegetables and foods of animal origin. H. pylori survives in complex foodstuffs such as milk, vegetables and ready-to-eat foods. This review article presents an overview of the present knowledge on the microbiological aspects in terms of phenotypic characteristics and growth requirements of H. pylori, focusing on the potential role that foodstuffs and water may play in the transmission of the pathogen to humans and the methods successfully used for the detection of this microorganism in foodstuffs and water.

show abstract

“…The genus Helicobacter was proposed by Goodwin et al (1989), and since then, the number of Helicobacter species has greatly increased encompassing more than 35 species (Mladenova-Hristova et al, 2017). This genus is divided into two groups: Gastric Helicobacter (GH) species, which constitute a third of all members of the genus.…”

Section: Introductionmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

View full text Add to dashboard Cite

This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml−1. Our findings suggest that Columbia or Brucella media, regardless of the use of hydrogen, can be used for the EHH isolation. In addition, the concentration of antibiotics included in commercial campylobacteria supplements is suitable for EHH species recovery. Significance and Impact of the Study Enterohepatic Helicobacter (EHH) infections have been associated with several diseases in humans such as acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases. Although they are frequently detected in clinical samples by molecular methods, only occasionally they are isolated using culture conditions described for the taxonomic related pathogen Campylobacter sp. This is because the optimal conditions for the isolation of EHH have not yet been described, which results in an underestimation of the prevalence and clinical importance of these emerging pathogens. Therefore, this study provides insight for culturing EHH species.

show abstract

Zoonotic potential of Helicobacter spp.

Cited by 60 publications

References 34 publications

First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows

First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows

Helicobacter pylori: A foodborne pathogen?

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Contact Info

Product

Resources

About