Zoonotic Potential and Antibiotic Resistance of &lt;i&gt;Escherichia coli&lt;/i&gt; in Neonatal Calves in Uruguay

Umpiérrez, Ana; Bado, Inés; Oliver, Martín; Acquistapace, Sofía; Etcheverría, Analía Inés; Padola, Nora Lía; Vignoli, Rafael; Zunino, Pablo

doi:10.1264/jsme2.me17046

Cited by 37 publications

(38 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with this result, the isolate D151-5 is trimethoprim and sulfonamide resistant and streptomycin susceptible. This gene cassette array dhfrA17-aadA5 was detected in Europe, Africa, Asia, and Australia from cattle in the last years (Stokes and Gillings, 2011; INTEGRALL database http://integrall.bio.ua.pt/), and this cassette array was recently identified in America from a calf in Uruguay (Umpiérrez et al ., 2017). The integron identified in the reference strain FV10191, harbored the cassette array dhfrA1-aadA1 which is frequently found in E. coli (Stokes and Gillings, 2011; INTEGRALL database http://integrall.bio.ua.pt/).…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and genotypic characterization of enterotoxigenic <i>Escherichia coli</i> isolated from diarrheic calves in Argentina

Pasayo¹,

Sanz²,

Padola³

et al. 2019

Open Vet J.

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) is the most common and global cause of neonatal calf diarrhea, but there is a little information regarding calf ETEC strains in Argentina. In this study, five ETEC isolates from diarrheic dairy calves (2–10 d old) from Buenos Aires and Cordoba, Argentina were characterized on the basis of virulence gene (VG) pattern, O:H serotyping, hemolytic phenotype, phylogenetic group affiliation, antimicrobial (AM) resistance profile, and presence of integron class 1 and 2. The five isolates were examined by polymerase chain reaction (PCR) for the presence of 18 bovine VGs and showed the following genotypes: F5 + /F41 + / sta + (D242), F5 + / sta + (D158), F5 + / sta + (D157), F5 + (D151-9), and F5 + / iucD + (D151-5). These VGs confer pathogenic potential and most of them are associated with the ETEC pathotype. The five isolates showed a non-hemolytic phenotype, belonged to five different serotypes: O101:H − , O141:H − , O60:H − , ONT:H10, and ONT:H − , and were assigned to the phylogenetic group A by the quadruplex Clermont PCR method. The AM resistance of the three isolates D242, D157, and D151-5 was determined by agar disk diffusion method for 24 AMs and they exhibited a multi-resistance phenotype (resistance to four different AM classes: Cephalosporins, Penicillins, Macrolides, and Ansamycins). In addition, class 1 integrons were found in the isolate D151-5 containing the dfrA17-aadA5 gene cassette and in the bovine ETEC reference strain FV10191 containing the dfrA1-aadA1 gene cassette. The present study revealed for the first time the occurrence of multi-resistant ETEC associated with neonatal diarrhea in dairy calves in Argentina. This finding may be used for diagnostic and therapeutic purposes.

show abstract

Section: Resultsmentioning

confidence: 99%

Phenotypic and genotypic characterization of enterotoxigenic <i>Escherichia coli</i> isolated from diarrheic calves in Argentina

Pasayo¹,

Sanz²,

Padola³

et al. 2019

Open Vet J.

View full text Add to dashboard Cite

show abstract

“…Shigatoxigenic E. coli induces apoptosis of intestinal cells and is associated with haemolytic-uraemic syndrome and haemorrhagic colitis in humans. Careless use of antibacterial agents in animals has led to the resistance of these E. coli strains to β-lactam antibiotics (ampicillin and amoxicillin with clavulanic acid), aminoglycosides (gentamicin), fluoroquinolones, or combined preparations containing trimethoprim (58). In recent years, the occurrence of coagulase-negative staphylococci (CNS) in the dairy cattle population has increased.…”

Section: Introductionmentioning

confidence: 99%

Non-antibiotic possibilities in prevention and treatment of calf diarrhoea

Smulski¹,

Turlewicz‐Podbielska

Wylandowska

et al. 2020

Journal of Veterinary Research

View full text Add to dashboard Cite

Due to increasing bacterial antibiotic resistance and the consumers’ tendency to choose organic products, cattle farmers are interested in alternative methods of calf diarrhoea treatment. This is a major challenge for veterinarians. Few methods of non-antibiotic treatment that bring satisfactory results have been reported in the related literature so far. In this article, the authors compare different non-antibiotic methods of diarrhoea prevention and treatment in calves. Among the alternatives discussed are herbs, probiotics, prebiotics and synbiotics, lactoferrin, and bacteriophages. It was found that the best results could be achieved through the use of pro-, pre- and synbiotics. However, the authors would like to point out that with the expansion of knowledge about the practical use of broad-scale bacteriophages, they could be the best alternative to antibiotics.

show abstract

“…Raw sewage is one of the major reservoirs of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) (45). Farm animals, including cows, pigs, and horses, can also harbor various ARB, some of which are pathogenic to humans (8, 33, 55). These ARB/ARGs contaminate surrounding and downstream environments (3, 30).…”

mentioning

confidence: 99%

Ecology of Pathogens and Antibiotic-resistant Bacteria in Environments: Challenges and Opportunities

Ishii

2019

Microb. Environ.

View full text Add to dashboard Cite

Various bacteria can cause human diseases. They spread directly from person to person or indirectly via various environmental matrixes, such as food and water. Major food-and waterborne pathogens include Campylobacter, Listeria, Salmonella, Shigella, Shiga toxin-producing Escherichia coli, Salmonella, Yersinia, and Vibrio (31). Most of these pathogens spread through the fecal-oral route. Their primary hosts include humans, farm animals (e.g., cows, pigs, and chickens), and wildlife (e.g., deer, birds). These hosts contribute to the spread of pathogens. For example, geese and other birds are known to harbor diverse Campylobacter (29, 40, 59) and Salmonella spp. (40). However, some of these pathogens also survive for long periods of time and even grow in environments such as water, soil, sediment, and algae (13, 22, 32), in many cases in association with or by forming biofilms (35, 36, 52). Since difficulties are associated with detecting various pathogens in a timely manner, the microbial quality of food and water has been monitored using so-called fecal indicator bacteria (FIB), such as E. coli and enterococci (22, 24). Although their primary habitats are the gastrointestinal tracts of warmblooded animals (18, 49), some FIB strains are more adapted to soil or other environments (22, 24, 37). Moreover, alternative FIB, such as Bacteroides, have been used to identify the occurrence of pathogens and their potential sources of contamination (28, 59). However, poor correlations have been reported between pathogen and FIB concentrations (23, 58), which limits the use of FIB for predicting the occurrence of pathogens. Some opportunistic pathogens, including Mycobacterium avium and Legionella pneumophila, are not of a fecal origin. These opportunistic pathogens use environments such as water distribution systems (11, 14, 15) and showerhead biofilms (12) as their primary habitats, and occasionally infect humans to cause diseases. Furthermore, various environmental bacteria have been reported as emerging pathogens. Among these, Arcobacter spp. are of great interest because this genus is frequently and abundantly detected in many wastewater treatment plants (10, 50). This genus is phylogenetically closely related to Campylobacter, but is metabolically more versatile and can grow at relatively low temperatures and with a wider range of O 2 concentrations (9). Some members of Arcobacter have also been reported to form symbiotic relationships with protists (16). A better understanding of the ecology of these environmental pathogens is essential for preventing their occurrence and spread (39, 47).

show abstract

Zoonotic Potential and Antibiotic Resistance of <i>Escherichia coli</i> in Neonatal Calves in Uruguay

Cited by 37 publications

References 63 publications

Phenotypic and genotypic characterization of enterotoxigenic <i>Escherichia coli</i> isolated from diarrheic calves in Argentina

Phenotypic and genotypic characterization of enterotoxigenic <i>Escherichia coli</i> isolated from diarrheic calves in Argentina

Non-antibiotic possibilities in prevention and treatment of calf diarrhoea

Ecology of Pathogens and Antibiotic-resistant Bacteria in Environments: Challenges and Opportunities

Contact Info

Product

Resources

About