Zonulin is a marker of epithelial and endothelial barrier functions in non-communicable diseases (narrative review)

Зыбина, Н. Н.; Nikonov, E. L.; Es, Gershteĭn; Маmedli, Z. Z.; Стилиди, И. С.; Kushlinskii, N. E.

doi:10.17116/dokgastro20221101128

Cited by 5 publications

(2 citation statements)

References 168 publications

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“…Further studies with a large number of patients are required to clarify the relationship between zonulin levels and the immune parameters of patients with IBD. There is also a limitation associated with the method of measuring blood zonulin, namely the commercially available ELISA kit, which quite likely detects not only zonulin but also other unknown representatives of the zonulin family [3]. In connection with these data, the use of improved laboratory kits with a more highly developed detection of specific and reliable monoclonal antibodies to zonulin is required in the future [13].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, much attention is being paid to the role of intestinal permeability in the pathogenesis of IBD and the study of biomarkers that are used for the diagnosis and determination of UC and CD activity. One of these physiological biomarkers is zonulin, a haptoglobin precursor involved in the physiological regulation of both the epithelial and endothelial barrier functions, facilitated by modulating intercellular tight junctions [3]. Its discovery over 20 years ago by A. Fasano et al has led to a better understanding of the complex mechanisms that regulate paracellular transport, as well as the role of intestinal permeability and the consistency of the intestinal barrier in maintaining homeostasis.…”

Section: Introductionmentioning

confidence: 99%

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The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Khusainova,

Genkel,

Kuznetsova

et al. 2024

Gastroenterology Insights

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The aim of the study was to investigate the relationship between the serum zonulin and the cellular immunity in patients with ulcerative colitis (UC) and Crohn’s disease (CD). The study included 97 patients, 13 (13.4%) patients with CD and 84 (86.6%) patients with UC. The concentration of zonulin in the serum was studied using the ELISA kits. The investigation of the circulated leukocyte subpopulation was carried out through flow cytometry. The functional activity of the circulating neutrophils was studied using the absorption capacity of the monodisperse polystyrene latex particles and indicators of oxygen-dependent metabolism in the nitroblue tetrazolium test. The serum zonulin concentration in CD patients was significantly higher compared with UC patients (p = 0.003). The zonulin concentration directly correlated with the functional activity of the circulating neutrophils. Patients with a zonulin concentration > 472.4 pg/mL had a significantly higher number of band neutrophils (p = 0.0104), CD3+CD8+ cells (p = 0.0212), NK cells (p = 0.0161), and lower–CD19+ cells (p = 0.0034). Among the IBD patients, zonulin was associated with IBD severity. An increase in the serum concentration of zonulin was associated with an increase in the functional activity of circulating neutrophils and an increase in the number of CD3+CD8+ cells, NK cells, and a decrease in the number of CD19+ cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Khusainova,

Genkel,

Kuznetsova

et al. 2024

Gastroenterology Insights

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Studying zonulin levels in patients with inflammatory bowel disease and peripheral arthritis

Khusainova,

Nesmeyanova,

Dolgushina

et al. 2023

HERALD of North-Western State Medical University named after I.

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BACKGROUND: Currently, disruption of the barrier function of the intestinal mucosa is considered one of the key mechanisms in the pathogenesis of extraintestinal manifestations of inflammatory bowel diseases, especially damage to the musculoskeletal system. AIM: To study the relationship between the level of zonulin in blood serum and the parameters of the immune status in patients with inflammatory bowel diseases in combination with peripheral arthritis. MATERIALS AND METHODS: 97 patients with ulcerative colitis and Crohn’s disease have been examined, with peripheral arthritis verified in 47 patients; the control group included 50 patients without peripheral arthritis. Zonulin, tumor necrosis factor α, interleukin-1β, -10, -17A, -23 were determined in blood serum using standard kits for direct enzyme immunoassay. RESULTS: Patients with peripheral arthritis were younger with an earlier onset of inflammatory bowel diseases (p 0.001). When assessing the concentration of serum zonulin in the patients with and without arthritis, no significant differences have been found. In the patients with peripheral arthritis, zonulin concentration directly correlated with the level of interleukin-17A (r = 0.323; p = 0.039), interleukin-23 (r = 0.321; p = 0.041). CONCLUSIONS: According to the data obtained, the use of zonulin as a marker of peripheral arthritis in inflammatory bowel diseases is not recommended. Nevertheless, the correlation of zonulin and the concentrations of interleukin-17А and -23 demonstrates its role in the regulation of mucosal immunity in the development of extraintestinal manifestations of inflammatory bowel diseases.

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Zonulin — regulation of tight contacts in the brain and intestine — facts and hypotheses

Naryzhny

Legina

2022

BIOMED KHIM

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In recent years, the interrelationship between the brain and the gut has become an area of high scientific interest. The intestine is responsible not only for digestion, as it contains millions of neurons, its own immune system, and affects the emotional and cognitive processes. The relationship between the gut and the brain suggests that the processes carried out by the gut microbiota play a significant role in the regulation of brain function, and vice versa. A special role here is played by intercellular tight junctions (TJ), where the zonulin protein holds an important place. Zonulin, an unprocessed precursor of mature haptoglobin, is the only physiological modulator of intercellular TJ that can reversibly regulate the permeability of the intestinal (IB) and blood-brain (BBB) barriers in the human body. BBB disruption and altered microbiota composition are associated with many diseases, including neurological disorders and neuroinflammation. That is, there is a gut-brain axis (GBA) — a communication system through which the brain modulates the functions of the gastrointestinal tract (GIT) and vice versa. GBA is based on neuronal, endocrine, and immunological mechanisms that are interconnected at the organismal, organ, cellular, and molecular levels.

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Zonulin is a marker of epithelial and endothelial barrier functions in non-communicable diseases (narrative review)

Cited by 5 publications

References 168 publications

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Studying zonulin levels in patients with inflammatory bowel disease and peripheral arthritis

Zonulin — regulation of tight contacts in the brain and intestine — facts and hypotheses

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