Zonula occludens-1 demonstrates a unique appearance in buccal mucosa over several layers

Imafuku, Kimiaki; Kamaguchi, Mayumi; Natsuga, Ken; Nakamura, Hideki; Shimizu, Hiroshi; Iwata, Hiroaki

doi:10.1007/s00441-021-03425-8

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…Human and mouse tissue samples were fixed with formalin and embedded in paraffin following dehydration. For morphological analysis, deparaffinized sections were stained with haematoxylin and eosin (H&E) by conventional methods 21 . Images of H&E‐stained sections were captured with a BZ‐9000 microscope (Keyence, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

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Zonula occludens‐1 distribution and barrier functions are affected by epithelial proliferation and turnover rates

et al. 2023

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Zonula occludens‐1 (ZO‐1) is a scaffolding protein of tight junctions, which seal adjacent epithelial cells, that is also expressed in adherens junctions. The distribution pattern of ZO‐1 differs among stratified squamous epithelia, including that between skin and oral buccal mucosa. However, the causes for this difference, and the mechanisms underlying ZO‐1 spatial regulation, have yet to be elucidated. In this study, we showed that epithelial turnover and proliferation are associated with ZO‐1 distribution in squamous epithelia. We tried to verify the regulation of ZO‐1 by comparing normal skin and psoriasis, known as inflammatory skin disease with rapid turnover. We as well compared buccal mucosa and oral lichen planus, known as an inflammatory oral disease with a longer turnover interval. The imiquimod (IMQ) mouse model, often used as a psoriasis model, can promote cell proliferation. On the contrary, we peritoneally injected mice mitomycin C, which reduces cell proliferation. We examined whether IMQ and mitomycin C cause changes in the distribution and appearance of ZO‐1. Human samples and mouse pharmacological models revealed that slower epithelial turnover/proliferation led to the confinement of ZO‐1 to the uppermost part of squamous epithelia. In contrast, ZO‐1 was widely distributed under conditions of faster cell turnover/proliferation. Cell culture experiments and mathematical modelling corroborated these ZO‐1 distribution patterns. These findings demonstrate that ZO‐1 distribution is affected by epithelial cell dynamics.

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Section: Methodsmentioning

confidence: 99%

“…For morphological analysis, deparaffinized sections were stained with haematoxylin and eosin (H&E) by conventional methods. 21 Images of H&E-stained sections were captured with a BZ-9000 microscope (Keyence, Tokyo, Japan).…”

Section: Histologymentioning

confidence: 99%

Zonula occludens‐1 distribution and barrier functions are affected by epithelial proliferation and turnover rates

et al. 2023

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“…However, the result was for reference, because the ELISA is not an established one. To confirm the precise autoantigen, we performed super‐resolution imaging by using a Nikon N‐SIM 4 and super‐resolution radial fluctuations (SRRF), which processed pictures taken by Keyence fluorescent microscopy. Briefly, a DIF sample was co‐stained with the anti‐BP180‐NC16A domain (primary Ab: TS39‐3; 5 secondary Ab: TRITC‐conjugated antimouse IgG) or the anti‐COL7‐NC1 domain (primary Ab: vWFA2; 6 secondary Ab: TRITC‐conjugated antirabbit IgG).…”

Section: Case Reportmentioning

confidence: 99%

“…However, the result was for reference, because the ELISA is not an established one. To confirm the precise autoantigen, we performed super-resolution imaging by using a Nikon N-SIM 4 domain (primary Ab: vWFA2; 6 secondary Ab: TRITC-conjugated antirabbit IgG). In N-SIM images, the deposition pattern of IgM autoantibodies (green) seems to be oriented parallel to that of BP180 (red) (Figure 2a).…”

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Super‐resolution imaging detects BP180 autoantigen in immunoglobulin M pemphigoid

Hirano

Iwata

Tsujuwaki

et al. 2021

The Journal of Dermatology

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The diagnoses of autoimmune blistering diseases, including bullous pemphigoid (BP) and pemphigus, are confirmed based on a combination of characteristic clinical findings, histological features, and immunological findings. Immunological tests are classified as direct immune fluorescence (DIF) tests, which detect tissue-bound autoantibodies, and serological tests, such as indirect immunofluorescence (IIF), enzyme-linked immunosorbent assays (ELISA), and immunoblotting. We previously investigated the tissue-bound immunoglobulin (Ig) isotypes in 100 cases of BP and found that 17% of patients demonstrated IgM deposits at the dermoepidermal junction (DEJ). 1 However, they showed IgG deposits as well as IgM, and there were no patients with only IgM autoantibodies. This is because IgG autoantibodies are mandatory for diagnosing BP. 2 Recently, the concept of IgM pemphigoid has been proposed. 3 We present a case showing histological subepidermal blistering associated with IgM deposits at the DEJ.

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“…Tissue homeostasis and regeneration is governed by a plethora of different signals, involving differentiation and growth factors, endocrine signals, and neuronal stimuli, which have been extensively studied in vitro and in vivo [2][3][4][5][6]. Above, the cells are in close contact with neighboring cells for the purpose of metabolic coupling and, in the case of epithelia, to enclose a defined physiological milieu [7,8]. Thus, a complex, three-dimensional environment is not only responsible for histogenesis during development, but also for tissue homeostasis and regeneration [9,10].…”

Section: Introductionmentioning

confidence: 99%

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

et al. 2021

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Among oral tissues, the periodontium is permanently subjected to mechanical forces resulting from chewing, mastication, or orthodontic appliances. Molecularly, these movements induce a series of subsequent signaling processes, which are embedded in the biological concept of cellular mechanotransduction (MT). Cell and tissue structures, ranging from the extracellular matrix (ECM) to the plasma membrane, the cytosol and the nucleus, are involved in MT. Dysregulation of the diverse, fine-tuned interaction of molecular players responsible for transmitting biophysical environmental information into the cell’s inner milieu can lead to and promote serious diseases, such as periodontitis or oral squamous cell carcinoma (OSCC). Therefore, periodontal integrity and regeneration is highly dependent on the proper integration and regulation of mechanobiological signals in the context of cell behavior. Recent experimental findings have increased the understanding of classical cellular mechanosensing mechanisms by both integrating exogenic factors such as bacterial gingipain proteases and newly discovered cell-inherent functions of mechanoresponsive co-transcriptional regulators such as the Yes-associated protein 1 (YAP1) or the nuclear cytoskeleton. Regarding periodontal MT research, this review offers insights into the current trends and open aspects. Concerning oral regenerative medicine or weakening of periodontal tissue diseases, perspectives on future applications of mechanobiological principles are discussed.

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Zonula occludens-1 demonstrates a unique appearance in buccal mucosa over several layers

Cited by 5 publications

References 38 publications

Zonula occludens‐1 distribution and barrier functions are affected by epithelial proliferation and turnover rates

Zonula occludens‐1 distribution and barrier functions are affected by epithelial proliferation and turnover rates

Super‐resolution imaging detects BP180 autoantigen in immunoglobulin M pemphigoid

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

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