Zonal variation of MRI-measurable parameters classifies cartilage degradation

Fleck, Allison K. M.; Krüger, Uwe; Carlson, Kyrsten; Waltz, Caitlin; McCallum, Scott A.; Lü, Xin; Wan, Leo Q.

doi:10.1016/j.jbiomech.2017.10.011

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…These results are similar to those of Fleck et al . who showed that sequential treatment of cABC followed by collagenase resulted in increased cartilage degradation compared to cABC or collagenase alone [15] . Therefore, the action of collagenase preceded by cABC may have a greater effect on collagen matrix degradation than collagenase alone [15] or than collagenase prior to cABC.…”

Section: Discussionmentioning

confidence: 99%

Selective Enzymatic Digestion of Proteoglycans and Collagens Alters Cartilage T1rho and T2 Relaxation Times

et al. 2018

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Our objective was to determine the relationship of T1rho and T2 relaxation mapping to the biochemical and biomechanical properties of articular cartilage through selective digestion of proteoglycans and collagens. Femoral condyles were harvested from porcine knee joints and treated with either chondroitinase ABC (cABC) followed by collagenase, or collagenase followed by cABC. Magnetic resonance (MR) images were acquired and cartilage explants were harvested for biochemical, biomechanical, and histological analyses before and after each digestion. Targeted enzymatic digestion of proteoglycans with cABC resulted in elevated T1rho relaxation times and decreased sulfated glycosaminoglycan (sGAG) content without affecting T2 relaxation times. In contrast, extractable collagen and T2 relaxation times were increased by collagenase digestion; however, neither was altered by cABC digestion. Aggregate modulus decreased with digestion of both components. Overall, we found that targeted digestion of proteoglycans and collagens had varying effects on biochemical, biomechanical, and imaging properties. T2 relaxation times were altered with changes in extractable collagen, but not changes in proteoglycan. However, T1rho relaxation times were altered with proteoglycan loss, which may also coincide with collagen disruption. Since it is unclear which matrix components are disrupted first in osteoarthritis, both markers may be important for tracking disease progression.

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Section: Discussionmentioning

confidence: 99%

Selective Enzymatic Digestion of Proteoglycans and Collagens Alters Cartilage T1rho and T2 Relaxation Times

et al. 2018

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“…Using experimental µMRI (7T, 17.6 µm×17.6 µm×1 mm), the authors found important synergistic effects of mechanical loading and depth-wise Gd-DTPA 2concentration on estimation of GAGs in healthy and degraded articular cartilage (Wang et al, 2013). The detection sensitivity of cartilage enzymatic degradation improved remarkably by performing zonal analysis using T1, T2, diffusion tensor imaging as well as gadolinium enhanced T1 (7T, 126 µm×126 µm×2 mm) (Fleck et al, 2017).…”

Section: Magnetic Resonance Imaging (Mri)mentioning

confidence: 99%

“…A minimum spatial resolution of 200 µm is therefore recommended. As this resolution should be applied outof-plane, application of MRI techniques would be limited although high field MRI (7T) achieves satisfactory in-the-plane resolution (Fleck et al, 2017). Visualization of the tissue deformation during the diffusion process is also challenging using fluorescentbased techniques.…”

Section: Tissue Deformationmentioning

confidence: 99%

Multi-scale imaging techniques to investigate solute transport across articular cartilage

Pouran

Arbabi

Bajpayee

et al. 2018

Journal of Biomechanics

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As articular cartilage is an avascular tissue, the transport of nutrients and cytokines through the tissue is essential for the health of cells, i.e. chondrocytes. Transport of specific contrast agents through cartilage has been investigated to elucidate cartilage quality. In laboratory, pre-clinical and clinical studies, imaging techniques such as magnetic imaging resonance (MRI), computed tomography (CT) and fluorescent microscopy have been widely employed to visualize and quantify solute transport in cartilage. Many parameters related to the physico-chemical properties of the solute, such as molecular weight, net charge and chemical structure, have a profound effect on the transport characteristics. Information on the interplay of the solute parameters with the imaging-dependent parameters (e.g. resolution, scan and acquisition time) could assist in selecting the most optimal imaging systems and data analysis tools in a specific experimental set up. Here, we provide a comprehensive review of various imaging systems to investigate solute transport properties in articular cartilage, by discussing their potentials and limitations. The presented information can serve as a guideline for applications in cartilage imaging and therapeutics delivery and to improve understanding of the set-up of solute transport experiments in articular cartilage.

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“…[ 36 ] Excellent image contrast is achieved for the deep layers of cartilage in the subtracted image, and the deep layers of cartilage are shown as a high signal line above the subchondral bone (arrows). [ 37 ]…”

Section: Onitoring the P Rocess Of Thementioning

confidence: 99%

Detection of Repair of the Zone of Calcified Cartilage with Osteoarthritis through Mesenchymal Stem Cells by Ultrashort Echo Time Magnetic Resonance Imaging

Zhou

et al. 2018

Chinese Medical Journal

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Objective:Currently, magnetic resonance imaging (MRI) is the most commonly used imaging modality for observing the growth and development of mesenchymal stem cells (MSCs) after in vivo transplantation to treat osteoarthritis (OA). However, it is a challenge to accurately monitor the treatment effects of MSCs in the zone of calcified cartilage (ZCC) with OA. This is especially true in the physiological and biochemical views that are not accurately detected by MRI contrast agents. In contrast, ultrashort time echo (UTE) MRI has been shown to be sensitive to the presence of the ZCC, creating the potential for more effectively observing the repair of the ZCC in OA by MSCs. A special focus is given to the outlook of the use of UTE MRI to detect repair of the ZCC with OA through MSCs. The limitations of the current techniques for clinical applications and future directions are also discussed.Data Sources:Using the combined keywords: “osteoarthritis”, “mesenchymal stem cells”, “calcified cartilage”, and “magnetic resonance imaging”, the PubMed/MEDLINE literature search was conducted up to June 1, 2017.Study Selection:A total of 132 published articles were initially identified citations. Of the 132 articles, 48 articles were selected after further detailed review. This study referred to all the important English literature in full.Results:In contrast, UTE MRI has been shown to be sensitive to the presence of the ZCC, creating the potential for more effectively observing the repair of the ZCC in OA by MSCs.Conclusions:The current studies showed that the ZCC could be described in terms of its histomorphology and biochemistry by UTE MRI. We prospected that UTE MRI has been shown the potential for more effectively observing the repair of the ZCC in OA by MSCs in vivo.

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Zonal variation of MRI-measurable parameters classifies cartilage degradation

Cited by 8 publications

References 30 publications

Selective Enzymatic Digestion of Proteoglycans and Collagens Alters Cartilage T1rho and T2 Relaxation Times

Selective Enzymatic Digestion of Proteoglycans and Collagens Alters Cartilage T1rho and T2 Relaxation Times

Multi-scale imaging techniques to investigate solute transport across articular cartilage

Detection of Repair of the Zone of Calcified Cartilage with Osteoarthritis through Mesenchymal Stem Cells by Ultrashort Echo Time Magnetic Resonance Imaging

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