Zona pellucida components are present in human fetal ovary before follicle formation

Törmälä, Reeta-Maria; Jääskeläinen, Minna; Lakkakorpi, Jouni; Liakka, Annikki; Tapanainen, Juha S.; Vaskivuo, Tommi

doi:10.1016/j.mce.2008.01.029

Cited by 13 publications

(10 citation statements)

References 25 publications

(26 reference statements)

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“…We confirmed over expression of two gap junction genes that are indispensable for the regulation of folliculogenesis and oogenesis: GJA1 ( Cx43 ) in GCs and GJA4 (Cx37) in oocytes. Moreover in our study, ZP2-4 were seen to significantly increase during preantral stages as shown in human fetal ovaries before the formation of zona pellicida [54]. …”

Section: Discussionsupporting

confidence: 59%

An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog

Bonnet

Cabau²,

Bouchez

et al. 2013

BMC Genomics

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BackgroundSuccessful early folliculogenesis is crucial for female reproductive function. It requires appropriate gene specific expression of the different types of ovarian cells at different developmental stages. To date, most gene expression studies on the ovary were conducted in rodents and did not distinguish the type of cell. In mono-ovulating species, few studies have addressed gene expression profiles and mainly concerned human oocytes.ResultsWe used a laser capture microdissection method combined with RNA-seq technology to explore the transcriptome in oocytes and granulosa cells (GCs) during development of the sheep ovarian follicle. We first documented the expression profile of 15 349 genes, then focused on the 5 129 genes showing differential expression between oocytes and GCs. Enriched functional categories such as oocyte meiotic arrest and GC steroid synthesis reflect two distinct cell fates. We identified the implication of GC signal transduction pathways such as SHH, WNT and RHO GTPase. In addition, signaling pathways (VEGF, NOTCH, IGF1, etc.) and GC transzonal projections suggest the existence of complex cell-cell interactions. Finally, we highlighted several transcription regulators and specifically expressed genes that likely play an important role in early folliculogenesis.ConclusionsTo our knowledge, this is the first comprehensive exploration of transcriptomes derived from in vivo oocytes and GCs at key stages in early follicular development in sheep. Collectively, our data advance our understanding of early folliculogenesis in mono-ovulating species and will be a valuable resource for unraveling human ovarian dysfunction such as premature ovarian failure (POF).

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Section: Discussionsupporting

confidence: 59%

An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog

Bonnet

Cabau²,

Bouchez

et al. 2013

BMC Genomics

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“…One of them (OLC12) expressed a zona pellucida-like structure, as can be seen in Figure 8. It has already been confirmed that ZP3 transcripts are expressed in the oocytes from early ovarian development, before the formation of follicles [31]. The function of ZP proteins during early fetal life is not clear, but the simultaneous expression of ZP3 suggests that it may have a role in the development of primordial follicles before zona pellucida formation.…”

Section: Resultsmentioning

confidence: 99%

Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods

Virant‐Klun

Skutella

Hren

et al. 2013

BioMed Research International

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The adult ovarian surface epithelium has already been proposed as a source of stem cells and germinal cells in the literature, therefore it has been termed the “germinal epithelium”. At present more studies have confirmed the presence of stem cells expressing markers of pluripotency in adult mammalian ovaries, including humans. The aim of this study was to isolate a population of stem cells, based on the expression of pluripotency-related stage-specific embryonic antigen-4 (SSEA-4) from adult human ovarian surface epithelium by two different methods: magnetic-activated cell sorting and fluorescence-activated cell sorting. Both methods made it possible to isolate a similar, relatively homogenous population of small, SSEA-4-positive cells with diameters of up to 4 μm from the suspension of cells retrieved by brushing of the ovarian cortex biopsies in reproductive-age and postmenopausal women and in women with premature ovarian failure. The immunocytochemistry and genetic analyses revealed that these small cells—putative stem cells—expressed some primordial germ cell and pluripotency-related markers and might be related to the in vitro development of oocyte-like cells expressing some oocyte-specific transcription factors in the presence of donated follicular fluid with substances important for oocyte growth and development. The stemness of these cells needs to be further researched.

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“…Furthermore, it has been indicated that ZP elements are found in human fetal ovaries prior to forming follicle. Finally, the researchers inferred that ZP components are found at the initial stages of ovarian development (Törmälä et al 2008). In our study, both OLCs and epithelial cells expressed ZP component.…”

Section: Discussionsupporting

confidence: 58%

“…Evidence indicated that in mice ZP1 is not vital, and ZP1 null animal form a ZP matrix which retains biological activity for fertilization and preimplantation development (Hoodbhoy et al 2006). Moreover, lower ZP1 mRNA expression has been reported in human fetal and mature ovaries (Törmälä et al 2008). It was also stated that ZP4 has a structural role in human oocyte and a minor function in sperm binding and induction of acrosome reaction (Canosa et al 2017).…”

Section: Discussionmentioning

confidence: 99%

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Supplemental Information 24: Cytoplasmic Immunostaining for vimentin.Raw Data

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Background. To study the effect of Bone morphogenetic protein 15 on differentiation potential of mesenchymal stem cell derived from human follicular fluid to oocyte like cell. Methods. Human FF derived cells were collected from 78 women in assisted fertilization program, and cultured in differentiation medium containing human recombinant BMP15 for 21 days. Mesenchymal stem cells and OLCs were characterized by real-time PCR and immunocytochemistry (ICC) staining. Results. MSCs expressed germ line stem cell markers, such as OCT4 and NANOG. After 15 days, OLCs formed and expressed zona pellucida markers (ZP2, ZP3), and reached 20-30 µm in diameters. Ten days after induction with BMP15, round cells remarkably developed, and the maximum size of OLCs reached 115 µm. Finally, a decrease ranging from 0.04 to 4.5 in the expression of pluripotency and oocyte specific markers was observed in the cells cultured in BMP15 supplemented medium. Our work demonstrates, FF derived MSCs have an innate potency to differentiate into OLCs, and BMP15 is effective in stimulating the differentiation of these cells, which may give an in vitro model to examine human germ cell development.

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Zona pellucida components are present in human fetal ovary before follicle formation

Cited by 13 publications

References 25 publications

An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog

An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog

Isolation of Small SSEA-4-Positive Putative Stem Cells from the Ovarian Surface Epithelium of Adult Human Ovaries by Two Different Methods

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