Zona pellucida-binding protein 2 (ZPBP2) and several proteins containing BX7B motifs in human sperm may have hyaluronic acid binding or recognition properties

Torabi, Forough; Bogle, O. A.; Estanyol, Josep Marı́a; Oliva, Rafael; Miller, D. H.

doi:10.1093/molehr/gax053

Cited by 22 publications

(15 citation statements)

References 48 publications

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“…Therefore, dysfunction in the ubiquitin-related pathway might have relevance in the physiological functions of the sperm. Furthermore, the effects of PLBD1 gene polymorphism on the biological function of the sperm is still unclear, even though it has been reported that PLBD1 is implicated in the fertilization processes [47]. Moreover, the PLBDI gene is related to 12 GO enriched terms, such as "lipid metabolic process", "signal transduction", and "kinase activity", suggesting the important role of the gene in various sperm biological functions.…”

Section: Snp Associations With Post-thaw Semen Qualitymentioning

confidence: 99%

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Mańkowska

Brym

Paukszto

et al. 2020

IJMS

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Genetic markers have been used to assess the freezability of semen. With the advancement in molecular genetic techniques, it is possible to assess the relationships between sperm functions and gene polymorphisms. In this study, variant calling analysis of RNA-Seq datasets was used to identify single nucleotide polymorphisms (SNPs) in boar spermatozoa and to explore the associations between SNPs and post-thaw semen quality. Assessment of post-thaw sperm quality characteristics showed that 21 boars were considered as having good semen freezability (GSF), while 19 boars were classified as having poor semen freezability (PSF). Variant calling demonstrated that most of the polymorphisms (67%) detected in boar spermatozoa were at the 3’-untranslated regions (3’-UTRs). Analysis of SNP abundance in various functional gene categories showed that gene ontology (GO) terms were related to response to stress, motility, metabolism, reproduction, and embryo development. Genomic DNA was isolated from sperm samples of 40 boars. Forty SNPs were selected and genotyped, and several SNPs were significantly associated with motility and membrane integrity of frozen-thawed (FT) spermatozoa. Polymorphism in SCLT1 gene was associated with significantly higher motility and plasma membrane integrity of FT spermatozoa from boars of the GSF group compared with those of the PSF group. Likewise, polymorphisms in MAP3K20, MS4A2, and ROBO1 genes were significantly associated with reduced cryo-induced lipid peroxidation and DNA damage of FT spermatozoa from boars of the GSF group. Candidate genes with significant SNP associations, including APPL1, PLBD1, FBXO16, EML5, RAB3C, OXSR1, PRICKLE1, and MAP3K20 genes, represent potential markers for post-thaw semen quality, and they might be relevant for future improvement in the selection procedure of boars for cryopreservation. The findings of this study provide evidence indicating that polymorphisms in genes expressed in spermatozoa could be considered as factors associated with post-thaw semen quality.

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Section: Snp Associations With Post-thaw Semen Qualitymentioning

confidence: 99%

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Mańkowska

Brym

Paukszto

et al. 2020

IJMS

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“…Sperm capacitation is a recognized phenomenon associated with acquisition of hyperactive motility comprising a complex set of highly regulated molecular and physiological events. As mature spermatozoa are no longer engaged in nuclear gene expression (Oliva, 2006; Jodar et al, 2013), understanding the role of protein modifiers, cell-cell communication and signaling pathways is essential (Baldi et al, 1996; Halet et al, 2003; De Jonge, 2005; Visconti et al, 2011; Buffone et al, 2012; Signorelli et al, 2012; Cuasnicú et al, 2016; Barrachina et al, 2018; Torabi et al, 2017b). For instance, many studies have correlated capacitation with an increase in cholesterol efflux from the sperm membrane (Visconti et al, 2002; Gadella et al, 2008) that modulates intracellular levels of calcium and bicarbonate ions (Visconti et al, 2011; Battistone et al, 2013; Li et al, 2014; Puga Molina et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Changes in Human Sperm During Sequential in vitro Capacitation and Acrosome Reaction

Castillo

Bogle

Jodar

et al. 2019

Front. Cell Dev. Biol.

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The male gamete is not completely mature after ejaculation and requires further events in the female genital tract to acquire fertilizing ability, including the processes of capacitation and acrosome reaction. In order to shed light on protein changes experienced by the sperm cell in preparation for fertilization, a comprehensive quantitative proteomic profiling based on isotopic peptide labeling and liquid chromatography followed by tandem mass spectrometry was performed on spermatozoa from three donors of proven fertility under three sequential conditions: purification with density gradient centrifugation, incubation with capacitation medium, and induction of acrosome reaction by exposure to the calcium ionophore A23187. After applying strict selection criteria for peptide quantification and for statistical analyses, 36 proteins with significant changes in their relative abundance within sperm protein extracts were detected. Moreover, the presence of peptide residues potentially harboring sites for post-translational modification was revealed, suggesting that protein modification may be an important mechanism in sperm maturation. In this regard, increased levels of proteins mainly involved in motility and signaling, both regulated by protein modifiers, were detected in sperm lysates following incubation with capacitation medium. In contrast, less abundant proteins in acrosome-reacted cell lysates did not contain potentially modifiable residues, suggesting the possibility that all those proteins might be relocated or released during the process. Protein-protein interaction analysis revealed a subset of proteins potentially involved in sperm maturation, including the proteins Erlin-2 (ERLIN2), Gamma-glutamyl hydrolase (GGH) and Transmembrane emp24 domain-containing protein 10 (TMED10). These results contribute to the current knowledge of the molecular basis of human fertilization. It should now be possible to further validate the potential role of the detected altered proteins as modulators of male infertility.

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“…25 However, in fact, the concentration of sp24666 was found to increase significantly from Cor 5 to Cor 6 in our study. P13611-associated proteins were highly expressed in spermatozoa that recognized the zona pellucida, 26 and the protein GO: 0009987 was highly expressed in highly active spermatozoa. 27 These proteins only increased 10-fold in the initial stage of the vas deferens transition from the cauda and were not highly expressed in the caput, corpus, and cauda.…”

Section: Discussionmentioning

confidence: 99%

Anatomic characteristics of epididymis based on histology, proteomic, and 3D reconstruction

Zhao

Zhai

et al. 2020

Andrology

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Background The epididymis is a popular research topic in urology and reproduction. Objectives To explore and identify the anatomical characteristics of the epididymis based on histology, proteomics, and 3D reconstruction of epididymal tubules. Materials and methods A 3D reconstruction of epididymal tubules was generated based on 7‐μm‐thick transverse serial sections of an epididymis. The proteins in the subcompartments of the epididymis were obtained and analyzed by non‐labeled sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH MS). Protein function, signaling pathways, protein expression, and the histology in different subcompartments were analyzed. Results The caput (Cap), corpus (Cor), and cauda (Cau) of the epididymis were divided into 6, 10, and 4 subcompartments, respectively, and the subcompartment between the Cap and Cor is mixed together. A total of 3411 proteins were identified, and 854 proteins were accurately quantified after screening. When the subcompartment Cap 5 transitioned to Cap 6 and Cap 6 to Cap 7, 87 and 52 proteins were upregulated and 14 and 7 proteins were downregulated, respectively. The Cor 9 transition to Cau 1 was marked by 230 proteins that were downregulated, while 74 proteins were upregulated. At the junction of the cauda and the vas deferens, 57 proteins were downregulated, and 410 proteins were upregulated. Cap 6 histology was consistent with that of Cor 1. Discussion and conclusion The epididymis contains distinct connective tissue septa that can be identified under a surgical tabletop microscope, enabling it to be divided into 20 subcompartments.

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Zona pellucida-binding protein 2 (ZPBP2) and several proteins containing BX7B motifs in human sperm may have hyaluronic acid binding or recognition properties

Cited by 22 publications

References 48 publications

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Proteomic Changes in Human Sperm During Sequential in vitro Capacitation and Acrosome Reaction

Anatomic characteristics of epididymis based on histology, proteomic, and 3D reconstruction

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