ZnS@ZIF-8 core-shell nanoparticles incorporated with ICG and TPZ to enable H₂S-amplified synergistic therapy

Abstract

Abnormal tumor microenvironment, such as hypoxia, interstitial hypertension and low pH, leads to unexpected resistance for current tumor treatment. The development of versatile drug delivery systems which present responsive characteristics to tumor microenvironment (TME) has been extensively carried out, but remains challenging. In this study, zeolitic imidazolate framework-8 (ZIF-8) coated ZnS nanoparticles have been designed and prepared for co-delivery of ICG/TPZ molecules, denoted as ZSZIT, for H 2 S-amplified synergistic therapy. Methods: The ZSZ nanoparticles were characterized using SEM, TEM and XRD. The in vitro viabilities of cancer cells cultured with ZSZIT under normoxia/hypoxia conditions were evaluated by cell counting kit-8 (CCK-8) assay. In addition, in vivo anti-tumor effect was also performed using male Balb/c nude mice as animal model. Results: ZSZIT shows cascade PDT and hypoxia-activated chemotherapeutic effect under an 808nm NIR irradiation. Meanwhile, ZSZIT degrades under tumor acidic environment, and H 2 S produced by ZnS cores could inhibit the expression of catalase, which subsequently favors the hypoxia and antitumor effect of TPZ drug. Both in vitro and in vivo studies demonstrate the H 2 S-sensitized synergistic antitumor effect based on cascade PDT/chemotherapy. Conclusion: This cascade H 2 S-sensitized synergistic nanoplatform has enabled more effective and lasting anticancer treatment.

Laser-triggered combined chemotherapy and photodynamic therapy enabled by iron sulfide-doxorubicin@bovine lactoferrin nanozymes for breast cancer therapy

“…Consistent with this nding, Shari , et al [6] and Shari , et al [8] showed that Dox focused more on breast cancer tissue using Fe NPs compared to the control group. However, the positive effect of using PDT on metallic NZs containing drug in tumor therapy is similar to the nding of Fang, et al [61] who showed that the effect of gas therapy to reduce drug resistance is very signi cant.…”

Photothermo‐Promoted Nanocatalysis Combined with H2S‐Mediated Respiration Inhibition for Efficient Cancer Therapy

“…H 2 S also could aggravate the hypoxia to activate hypoxia‐activated prodrug to enhance chemotherapy. [ 17 ] Additionally, H 2 S gas is reported to induce acute toxicity by inhibiting the activity of mitochondrial cytochrome c oxidase (COX IV). [ 18 ] COX IV is the terminal metal membrane enzyme on the electron transport chain of the mitochondrial inner membrane, and an important part of cellular aerobic respiration and generation of adenosine triphosphate (ATP).…”

Lanthanide Europium MOF Nanocomposite as the Theranostic Nanoplatform for Microwave Thermo-Chemotherapy and Fluorescence Imaging

“…Here, to solve this problem, a stable zeolite imidazole framework structure was introduced on the surface of Eu MOF to provide coating protection for EuMOF, while as an excellent drug loading tool due to its porosity and high speci c surface [44][45][46]. Therefore, we reported a luminescent EuMOF nanocomposite (EuMOF@ZIF/AP-PEG, named EZAP) as a multifunctional nanoplatform for microwave-thermal therapy and chemotherapy and uorescence imaging of tumors (Scheme 1).…”