ZnO Nanocrystals Shuttled By Extracellular Vesicles As Effective Trojan nano-horses Against Cancer Cells

Dumontel, Bianca; Susa, Francesca; Limongi, Tania; Canta, Marta; Racca, Luisa; Chiodoni, Angelica; Garino, Nadia; Chiabotto, Giulia; Centomo, Maria Laura; Pignochino, Ymera; Cauda, Valentina

doi:10.2217/nnm-2019-0231

Cited by 45 publications

(39 citation statements)

References 42 publications

(67 reference statements)

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“…A different distribution of treatments and number of shots could also be tested in order to maximize the synergy. Since the ZnO specific toxicity toward cancer cells is reported in the literature (Racca et al, 2018;Dumontel et al, 2019), additional tests with ZnO NCs and SW on healthy cells could be performed to confirm the selectivity of the proposed anticancer approach before to proceed with in vivo investigations.…”

Section: Future Overviewmentioning

confidence: 99%

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Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

Racca

Limongi

Vighetto

et al. 2020

Front. Bioeng. Biotechnol.

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In the last years, different nanotools have been developed to fight cancer cells. They could be administered alone, exploiting their intrinsic toxicity, or remotely activated to achieve cell death. In the latter case, ultrasound (US) has been recently proposed to stimulate some nanomaterials because of the US outstanding property of deep tissue penetration and the possibility of focusing. In this study, for the first time, we report on the highly efficient killing capability of amino-propyl functionalized ZnO nanocrystals (ZnO NCs) in synergy with high-energy ultrasound shock waves (SW) for the treatment of cancer cells. The cytotoxicity and internalization of ZnO NCs were evaluated in cervical adenocarcinoma KB cells, as well as the safety of the SW treatment alone. Then, the remarkably high cytotoxic combination of ZnO NCs and SW was demonstrated, comparing the effect of multiple (3 times/day) SW treatments toward a single one, highlighting that multiple treatments are necessary to achieve efficient cell death. At last, preliminary tests to understand the mechanism of the observed synergistic effect were carried out, correlating the nanomaterial surface chemistry to the specific type of stimulus used. The obtained results can thus pave the way for a novel nanomedicine treatment, based on the synergistic effect of nanocrystals combined with highly intense mechanical pressure waves, offering high efficiency, deep and focused tissue penetration, and a reduction of side effects on healthy cells.

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Section: Future Overviewmentioning

confidence: 99%

“…An additional enhancement could be generated by improving the colloidal dispersibility of ZnO NCs (Dumontel et al, 2019;Limongi et al, 2019). These interesting features could be successfully exploited for a future application of ZnO NCs and SW in in vivo investigations and clinic.…”

Section: Future Overviewmentioning

confidence: 99%

Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

Racca

Limongi

Vighetto

et al. 2020

Front. Bioeng. Biotechnol.

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“…They demonstrated that the coated MOF NPs were taken up within HeLa cells, enabling delivery of the anticancer drug suberoyl bishydroxamic acid. Dumontel et al coated zinc oxide nanocrystals (ZnO NCs) with EVs derived from KB (CCL17) cancer cells 37 . They evaluated various parameters for encapsulation within EVs, including the coupling temperature and time, mixing method, type of dispersing medium, and ratio between EVs and ZnO NCs.…”

Section: Extracellular Vesicles As Versatile Coatings For Nanoparticlesmentioning

confidence: 99%

Extracellular vesicle‐coated nanoparticles

Fathi

Rao

Chen

2020

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Synthetic nanoparticles have been used for a variety of theranostic applications to aid in the betterment of human health. These nanoparticles can provide platforms for targeted imaging and therapy of diseased tissues. The development of surface coatings for nanoparticles has enabled their selective uptake in tissues of interest, and the use of membrane‐derived nanoparticle coatings provides a particularly promising approach for the regulation of nanoparticle‐tissue interactions. Membranous extracellular vesicles (EVs) secreted by cells have been known to contain antigens, proteins, and other cell components on their surface that facilitate their uptake in target cells, enabling the transport of information from one cell to another. EV‐based nanoparticle coatings allow for the expansion of nanoparticle targeting from typical approaches that target individual antigens, to an approach that can simultaneously target many antigens for more efficient uptake within target cells. EV‐derived coatings also possess immune evasive properties that can lead to increased circulation time. In this mini‐review, we describe the various approaches and applications for EV coating of nanoparticles, a majority of which focus on cancer applications. We also provide an overview of commonly used EV sources for nanoparticle coating applications.

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“…A new nanoconstruct exploits the peculiar features of zinc oxide NPs (ZnO NPs) to treat cancer without the addition of drugs. Zinc oxide NPs encapsulated inside the EVs can be efficiently internalized by cancer cells causing their apoptosis [122]. A summary of cargo-loaded EVs with the related bibliographic references is provided in Table 2.…”

Section: Loading Nanotechnological Modification Into Evsmentioning

confidence: 99%

Engineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine

Susa

Limongi

Dumontel

et al. 2019

Cancers

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Fast diagnosis and more efficient therapies for cancer surely represent one of the huge tasks for the worldwide researchers’ and clinicians’ community. In the last two decades, our understanding of the biology and molecular pathology of cancer mechanisms, coupled with the continuous development of the material science and technological compounds, have successfully improved nanomedicine applications in oncology. This review argues on nanomedicine application of engineered extracellular vesicles (EVs) in oncology. All the most innovative processes of EVs engineering are discussed together with the related degree of applicability for each one of them in cancer nanomedicines.

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ZnO Nanocrystals Shuttled By Extracellular Vesicles As Effective Trojan nano-horses Against Cancer Cells

Cited by 45 publications

References 42 publications

Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

Extracellular vesicle‐coated nanoparticles

Engineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine

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