ZnO films formed by atomic layer deposition as an optical biosensor platform for the detection of Grapevine virus A-type proteins

Abstract

Novel sensitive optical biosensor for determination of Grapevine virus A-type (GVA) proteins (GVA-antigens) has been designed. This biosensor was based on thin films of Zinc Oxide (ZnO) deposited by atomic layer deposition (ALD). The ZnO-based films have demonstrated favorable surface-structural properties for the direct immobilization of antibodies against GVA-antigens in order to form a biosensitive layer sensitive to GVA-antigens. The immobilization was confirmed by intensity changes in the main near band emission (NBE) peak of ZnO and by the formation of intense photoluminescence band, discovered in the visible range around 425nm, caused by the immobilized proteins. The GVA-antigen detection was performed by the evaluation of changes and behavior of a corresponding luminescence band. The sensitivity of as-formed label-free biosensor towards the GVA-antigens was determined in the range from 1pg/ml to 10ng/ml; in addition, the selectivity of biosensor was evaluated.

Zinc oxide nanorod based immunosensing platform for the determination of human leukemic cells

“…Many efforts have been recently made to construct ZnO-based biosensors with high performance [11,12]. Corso et al [13] developed an effective antibody immobilization technique that imparts sensitivity and specificity to the ZnO-based devices.…”

Interaction mechanism between TiO2 nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors

“…[5][6][7] A good biocompatibility of TiO 2 nanostructures, their applicability at physiological pHs in the range of $5.5-7.0, non-toxicity and excellent chemical stability have resulted in the extensive application of TiO 2 in electrochemical, 8-10 electrical 11 and optical [12][13][14] biosensors. Optical biosensors are increasingly studied class of biosensors because they allow to evaluate some inter-molecular interactions contactless 15 and without any chemical/physical labels. 3 The development of immunosensors, based on the optical detection methods, that can be applied for the determination of large variety of analytes in the complex biological samples 7,12,14,15 is of great interest.…”

“…Optical biosensors are increasingly studied class of biosensors because they allow to evaluate some inter-molecular interactions contactless 15 and without any chemical/physical labels. 3 The development of immunosensors, based on the optical detection methods, that can be applied for the determination of large variety of analytes in the complex biological samples 7,12,14,15 is of great interest.…”

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

“…ZnO, a wide band gap of 3.37 eV semiconductor with large excitation binding energy of 60 meV at room temperature, has been widely investigated due to its potential wide-ranging applications [22][23][24], such as photocatalyst, solar cell, PEC sensor, nanogenerater ect.…”