ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer

Bhat, Sevitha; Kabekkodu, Shama Prasada; Adiga, Divya; Fernandes, Rayzel C.; Shukla, Vaibhav; Bhandari, Poonam; Pandey, Deeksha; Sharan, Krishna; Satyamoorthy, Kapaettu

doi:10.1007/s10565-021-09582-4

Cited by 27 publications

(20 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ZNF281 has been proved to play a crucial role in controlling cellular stemness expression and EMT by EMT induction and regulation of EMT-associated gene expression in colorectal cancer [30]. ZNF471 suppressed cervical cancer cell invasion through modulating EMT by negatively regulating Wnt/ β-catenin signaling pathway [31]. In our study, we observed that ZNF677 signi cantly reduced 786-0 cell invasion.…”

Section: Discussionsupporting

confidence: 58%

Over-Expression of ZNF677 Inhibits Proliferation, Invasion and Induces Apoptosis in Clear Cell Renal Cell Carcinoma 786-0 Cells

Liang

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: ZNF677 has been reported downregulation in several cancers due to DNA methylation. Recent studies have demonstrated that ZNF677 acts as a tumor suppressor gene in cancer. However, the expression and function of ZNF677 in ccRCC is still unclear. In this study, we aimed to investigate the expression of ZNF677 in ccRCC tissues and the malignant biological behavior of ZNF677 on 786-0 cells. Methods: Combining with bioinformatics analysis,qRT-PCR and IHC analysis was carried out to detect ZNF677 expression in 60 Clear cell Renal cell carcinoma (ccRCC) tissues and 47 normal tissues and 786-0 cells. In vitro, lentiviral vector containing negative control and ZNF677 vector was constructed and transfected into 786-0 cells, respectively. CCK8 and clone formation assays were used to measure the proliferation of 786-0 cells, and apoptosis was assessed by flow cytometry assay, and transwell assay was carried out to measure the invasion ability of 786-0 cells. Apoptosis markers and EMT markers expression was evaluated by western blot. Results: ZNF677 is hypermethylated in ccRCC and associated with clinicopathologic features,and functional assays suggested that ZNF677 inhibits tumor cell proliferation, invasion and induce apoptosis. Further prognosis analysis indicated that low expression of ZNF677 was associated with shorter overall survival (OS) and ZNF677 suppressed invasion and EMT of 786-0 cells through activating PI3K/AKT signaling pathway, but the exact mechanism remains unknown. Conclusions: ZNF677 can be used as an adverse prognostic biomarker and potential therapeutic target for ccRCC.

show abstract

Section: Discussionsupporting

confidence: 58%

Over-Expression of ZNF677 Inhibits Proliferation, Invasion and Induces Apoptosis in Clear Cell Renal Cell Carcinoma 786-0 Cells

Liang

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Zinc finger proteins play crucial roles in transcriptional and post-transcriptional regulation of immune response [ 45 ] and are involved in cancer development and progression [ 46 ]. Among the identified zinc finger proteins in our study, ZNF471 functions as a tumor suppressor in several cancers and is frequently hypermethylated in tumor tissues [ 47 , 48 , 49 , 50 , 51 ]. Higher levels of ZNF577 methylation in leukocytes have been associated with obesity [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Epigenome-Wide Association Study of Prostate Cancer in African Americans Identifies DNA Methylation Biomarkers for Aggressive Disease

Tsai

Chang

et al. 2021

Biomolecules

View full text Add to dashboard Cite

DNA methylation plays important roles in prostate cancer (PCa) development and progression. African American men have higher incidence and mortality rates of PCa than other racial groups in U.S. The goal of this study was to identify differentially methylated CpG sites and genes between clinically defined aggressive and nonaggressive PCa in African Americans. We performed genome-wide DNA methylation profiling in leukocyte DNA from 280 African American PCa patients using Illumina MethylationEPIC array that contains about 860K CpG sties. There was a slight increase of overall methylation level (mean β value) with the increasing Gleason scores (GS = 6, GS = 7, GS ≥ 8, P for trend = 0.002). There were 78 differentially methylated CpG sites with P < 10−4 and 9 sites with P < 10−5 in the trend test. We also found 77 differentially methylated regions/genes (DMRs), including 10 homeobox genes and six zinc finger protein genes. A gene ontology (GO) molecular pathway enrichment analysis of these 77 DMRs found that the main enriched pathway was DNA-binding transcriptional factor activity. A few representative DMRs include HOXD8, SOX11, ZNF-471, and ZNF-577. Our study suggests that leukocyte DNA methylation may be valuable biomarkers for aggressive PCa and the identified differentially methylated genes provide biological insights into the modulation of immune response by aggressive PCa.

show abstract

“…A further example includes ZNF471, which acts as a tumor suppressor in cervical cancer. Hypermethylation of the ZNF471 gene promoter and low ZNF471 expression was correlated with poor overall and recurrence-free survival in cervical cancer (Bhat et al, 2021).…”

Section: Introductionmentioning

confidence: 95%

Comprehensive Analysis of a Zinc Finger Protein Gene–Based Signature with Regard to Prognosis and Tumor Immune Microenvironment in Osteosarcoma

2022

View full text Add to dashboard Cite

Osteosarcoma is the most common malignant bone tumor that seriously threatens the lives of teenagers and children. Zinc finger (ZNF) protein genes encode the largest transcription factor family in the human genome. Aberrant expressions of ZNF protein genes widely occur in osteosarcoma, and these genes are therefore attractive biomarker candidates for prognosis prediction. In this study, we conducted a comprehensive analysis of ZNF protein genes in osteosarcoma and identified prognosis-related ZNF protein genes. Then, we constructed a prognostic signature based on seven prognosis-related ZNF protein genes and stratified patients into high- and low-risk groups. The seven genes included MKRN3, ZNF71, ZNF438, ZNF597, ATMIN, ZNF692, and ZNF525. After validation of the prognostic signature in internal and external cohorts, we constructed a nomogram including clinical features such as sex and age and the relative risk score based on the risk signature. Functional enrichment analysis of the risk-related differentially expressed genes revealed that the prognostic signature was closely associated with immune-related biological processes and signaling pathways. Moreover, we found significant differences between the high- and low-risk groups for the scores of diverse immune cell subpopulations, including CD8+ T cells, neutrophils, Th1 cells, and TILs. Regarding immune function, APC co-inhibition, HLA, inflammation promotion, para-inflammation, T-cell co-inhibition, and the type I IFN response were significantly different between the high- and low-risk groups. Of the seven ZNF protein genes, lower expressions of ATMIN, MKRN3, ZNF71, ZNF438, and ZNF597 were correlated with a high risk, while higher expressions of ZNF525 and ZNF692 were associated with a high risk. The Kaplan–Meier survival analysis suggested that lower expressions of ATMIN, ZNF438, and ZNF597 and the higher expression of ZNF692 were associated with worse overall survival in osteosarcoma. In conclusion, our ZNF protein gene–based signature was a novel and clinically useful prognostic biomarker for osteosarcoma patients.

show abstract

ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer

Cited by 27 publications

References 48 publications

Over-Expression of ZNF677 Inhibits Proliferation, Invasion and Induces Apoptosis in Clear Cell Renal Cell Carcinoma 786-0 Cells

Over-Expression of ZNF677 Inhibits Proliferation, Invasion and Induces Apoptosis in Clear Cell Renal Cell Carcinoma 786-0 Cells

Epigenome-Wide Association Study of Prostate Cancer in African Americans Identifies DNA Methylation Biomarkers for Aggressive Disease

Comprehensive Analysis of a Zinc Finger Protein Gene–Based Signature with Regard to Prognosis and Tumor Immune Microenvironment in Osteosarcoma

Contact Info

Product

Resources

About