ZNF24 is upregulated in prostate cancer and facilitates the epithelial‑to‑mesenchymal transition through the regulation of Twist1

Huang, Xiaohong; Liu, Nanxin; Xiong, Xia

doi:10.3892/ol.2020.11456

Cited by 10 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the higher the level of ZNF71 the better the survival of NSCLC patients, which is similar to our results that high levels of ZNF24 expression in NLSCLC patients were significantly associated with longer survival in all stages. Furthermore, recent studies have shown that ZNF24 is a tumor suppressor gene for HCC (23,37). These suggest that it is of clinical significance to elucidate the molecular mechanism of how ZNF24 functions as a tumor suppressor in NSCLC.…”

Section: Discussionmentioning

confidence: 95%

RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence

2021

View full text Add to dashboard Cite

ObjectiveUnderstanding the characteristics of tumor suppressor genes (TSGs) is of great significance for the development of new targeted treatment strategies for non-small cell lung cancer (NSCLC). Therefore, this present article is to explore the underlying molecular mechanism of ZFN24 inhibiting the development of NSCLC.MethodsWe performed RT-PCR and Western blotting for evaluating associated RNA and protein expression. CCK8, colony forming and sphere-forming assays were used to evaluate the proliferation and stemness of NSCLC cells. NSCLC cell senescence was examined by β-galactosidase staining assay. Luciferase assay was performed to evaluate β-catenin transcriptional activity. The effect of ZNF24 on NSCLC cells in vivo was evaluated by the xenograft tumor experiment.ResultsEctopic expression of ZNF24 significantly inhibited cell viability, colony forming ability, and stemness of NSCLC cells. WNT signaling pathway was inhibited by ZNF24 resulting in NSCLC cell senescence. β-catenin transcriptional activity was significantly inhibited by ZNF24 (P < 0.05). Ectopic expression of ZNF24 significantly inhibited xenotransplant tumors growth in vivo (P < 0.05).ConclusionZNF24 could notably inhibit the development of NSCLC by inhibiting the WNT signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 95%

RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence

2021

View full text Add to dashboard Cite

show abstract

“…In this study, the high ZNF24 expression was detected within para-carcinoma samples, while low expression was detected in THCA tumor tissues in all THCA patients and demonstrated tumor suppressor function of ZNF24 in THCA. Of note, several recent studies have shown that ZNF24 is the tumor suppressor gene of HCC (12,22). This indicated that elucidating the molecular mechanism underlying ZNF24 tumor suppressor function in THCA had clinical significance.…”

Section: Discussionmentioning

confidence: 98%

The Novel Tumor Suppressor Gene ZNF24 Induces THCA Cells Senescence by Regulating Wnt Signaling Pathway, Resulting in Inhibition of THCA Tumorigenesis and Invasion

et al. 2021

View full text Add to dashboard Cite

ObjectClinically, the effective treatment options available to thyroid cancer (THCA) patients are very limited. Elucidating the features of tumor suppressor genes (TSGs) and the corresponding signal transduction cascade may provide clues for the development of new strategies for targeted therapy of THCA. Therefore, this paper aims to explore the mechanism of ZNF24 underlying promoting THCA cell senescence at molecular level.MethodsWe performed RT-PCR and Western Blotting for evaluating associated RNA and protein expression. CCK8, colony forming, wound healing and Transwell chamber assays were conducted to examine THCA cell proliferation, invasion and migration. β-galactosidase staining assay was performed to detect THCA cells senescence. The size and volume of xenotransplanted tumors in nude mice are calculated to asses ZNF24 effect in vivo.ResultsEctopic expression of ZNF24 significantly inhibited the cell viability, colony forming, migration and invasion abilities of THCA cell lines (K1/GLAG-66i and BCPAPi) (P < 0.05). ZNF24 induced BCPAPi cells senescence through regulating Wnt signaling pathway. ZNF24 inhibited Wnt signaling pathway activition by competitively binding β-catenin from LEF1/TCF1-β-catenin complex. In nude mice, both Ectopic expression of ZNF24 and 2,4-Da (the strong β-catenin/Tcf-4 inhibitor) treatment significantly decreased both the size and weight of xenotransplanted tumors when compared with control mice (P < 0.05).ConclusionResults obtained in vivo and in vitro reveal the role of ZNF24 in significantly suppressing THCA tumorigenesis and invasion by regulating Wnt signaling pathway.

show abstract

“…ZNF24 functioned as a negative regulator of tumor development in breast cancer and gastric cancer [30,31]. ZNF24 also acted as an oncogene and promoted EMT of prostate cancer cells [32]. However, mechanisms of these genes in IBC still need extensive experimental research to reveal.…”

Section: Discussionmentioning

confidence: 99%

CASP9 As a Prognostic Biomarker and Promising Drug Target Plays a Pivotal Role in Inflammatory Breast Cancer

Zhang

Wang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background : Inflammatory breast cancer (IBC) is one of the most rare and aggressive subtype of primary breast cancer (BC). Our study aimed to explore hub genes related to the pathogenesis of IBC, which could be considered as novel molecular biomarkers for IBC diagnosis and prognosis. Material and Methods: Two datasets from gene expression omnibus database (GEO) were selected. Enrichment analysis and protein-protein interaction (PPI) network for the DEGs were performed. We analyzed the prognostic values of hub genes in the Kaplan–Meier Plotter. Connectivity Map (CMap) and Comparative Toxicogenomics Database (CTD) was used to find candidate small molecules capable to reverse the gene status of IBC. Results : 157 DEGs were selected in total. We constructed the PPI network with 154 nodes interconnected by 128 interactions. KEGG pathway analysis indicated that the DEGs were enriched in apoptosis, pathways in cancer and insulin signaling pathway. PTEN, PSMF1, PSMC6, AURKB, FZR1, CASP9, CASP6, CASP8, BAD, AKR7A2, ZNF24, SSX2IP, SIGLEC1, MS4A4A and VSIG4 were selected as hub genes based on the high degree of connectivity. Six hub genes (PSMC6, AURKB, CASP9, BAD, ZNF24 and SSX2IP) that were significantly associated with the prognosis of breast cancer. The expression of CASP9 protein was associated with prognosis and immune cells infiltration of breast cance. CASP9- naringenin (NGE) is expected to be the most promising candidate gene-compound interaction for the treatment of IBC. Conclusion : Taken together, CASP9 can be used as a prognostic biomarker and a novel therapeutic target in IBC.

show abstract

ZNF24 is upregulated in prostate cancer and facilitates the epithelial‑to‑mesenchymal transition through the regulation of Twist1

Cited by 10 publications

References 32 publications

RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence

RETRACTED: A Novel Tumor Suppressor Gene, ZNF24, Inhibits the Development of NSCLC by Inhibiting the WNT Signaling Pathway to Induce Cell Senescence

The Novel Tumor Suppressor Gene ZNF24 Induces THCA Cells Senescence by Regulating Wnt Signaling Pathway, Resulting in Inhibition of THCA Tumorigenesis and Invasion

CASP9 As a Prognostic Biomarker and Promising Drug Target Plays a Pivotal Role in Inflammatory Breast Cancer

Contact Info

Product

Resources

About