Zn2+, derived from cell preparation, partly attenuates Ca2+-dependent cell death induced by A23187, calcium ionophore, in rat thymocytes

Sakanashi, Yoko; Oyama, Tomohiro M.; Matsuo, Yuki; Oyama, Toshihisa B.; Nishimura, Yumiko; Ishida, Shiro; Imai, Shoji; Okano, Yoshiro; Oyama, Yasuo

doi:10.1016/j.tiv.2008.12.006

Cited by 41 publications

(28 citation statements)

References 34 publications

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“…The beaker containing the cell suspension was incubated in a water bath at 36-37°C for 1 hr before the experiment. Although the Tyrode's solution did not contain ZnCl 2 , the cell suspension contained 200-230 nM zinc derived from the cell preparation (Sakanashi et al, 2009).…”

Section: Animals and Cell Preparationmentioning

confidence: 99%

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

et al. 2013

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-It was recently reported that triclocarban was absorbed significantly from soap used during showering in human subjects and that its C max in their whole blood ranged from 23 nM to 530 nM. We revealed that a nanomolar concentration (300 nM) of triclocarban potentiated the cytotoxicity of 300 μM H 2 O 2 in rat thymocytes by using cytometric techniques with appropriate fluorescent probes. Although 300 nM triclocarban did not itself increase the population of dead cells (cell lethality), it facilitated the process of cell death induced by H 2 O 2 , resulting in a further increase in the population of dead cells. Nanomolar concentrations (300 nM or higher) of triclocarban significantly decreased the cellular content of nonprotein thiol (glutathione), which has a protective role against oxidative stress. Triclocarban at 300 nM or higher increased the cell vulnerability to oxidative stress. The results may suggest that nanomolar concentration (300 nM or higher) of triclocarban affects some cellular functions although there is no evidence for adverse effects of triclocarban in humans at present.

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Section: Animals and Cell Preparationmentioning

confidence: 99%

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

et al. 2013

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“…The beaker containing the cell suspension was water-bathed at 36-37°C for 1 h before the experiment. Although the chemical composition of Tyrode's solution did not contain ZnCl 2 , the cell suspension generally contained 200-230 nM zinc derived from the cell preparation [31].…”

Section: Animals and Cell Preparationmentioning

confidence: 99%

Putative role of intracellular Zn2+ release during oxidative stress: a trigger to restore cellular thiol content that is decreased by oxidative stress

et al. 2011

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Although the ability of zinc to retard the oxidative process has been recognized for many years, zinc itself has been reported to induce oxidative stress. In order to give some insights into elucidating the role of intracellular Zn(2+) in cells suffering from oxidative stress, the effects of N-ethylmaleimide (NEM) and ZnCl(2) on cellular thiol content and intracellular Zn(2+) concentration were studied by use of 5-chloromethylfluorescein diacetate (5-CMF-DA) and FluoZin-3 pentaacetoxymethyl ester (FluoZin-3-AM) in rat thymocytes. The treatment of cells with NEM attenuated 5-CMF fluorescence and augmented FluoZin-3 fluorescence in a dose-dependent manner. These NEM-induced phenomena were observed under external Zn(2+)-free conditions. Results suggest that NEM decreases cellular thiol content and induces intracellular Zn(2+) release. Micromolar ZnCl(2) dose-dependently augmented both FluoZin-3 and 5-CMF fluorescences, suggesting that the elevation of intracellular Zn(2+) concentration increases cellular thiol content. Taken together, it is hypothesized that intracellular Zn(2+) release during oxidative stress is a trigger to restore cellular thiol content that is decreased by oxidative stress.

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“…The cell suspension was incubated at 36-37°C for 1 h before the experiment. Importantly, the zinc concentration in Tyrode's solution was 32.4 ± 4.0 nM in the case of Tyrode's solution [40]. This significant increase in zinc concentration was probably due to the reagents, probably containing very trace zinc, that were used to prepare the solution.…”

Section: Animals and Cell Preparationmentioning

confidence: 96%

Dithiocarbamate fungicides increase intracellular Zn2+ levels by increasing influx of Zn2+ in rat thymic lymphocytes

Kanemoto-Kataoka

Oyama

Ishibashi

et al. 2015

Chemico-Biological Interactions

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Zn2+, derived from cell preparation, partly attenuates Ca2+-dependent cell death induced by A23187, calcium ionophore, in rat thymocytes

Cited by 41 publications

References 34 publications

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

Nanomolar concentration of triclocarban increases the vulnerability of rat thymocytes to oxidative stress

Putative role of intracellular Zn2+ release during oxidative stress: a trigger to restore cellular thiol content that is decreased by oxidative stress

Dithiocarbamate fungicides increase intracellular Zn2+ levels by increasing influx of Zn2+ in rat thymic lymphocytes

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