ZmXTH, a xyloglucan endotransglucosylase/hydrolase gene of maize, conferred aluminum tolerance in Arabidopsis

Du, Hanmei; Hu, Xiaoqi; Yang, Weng Lang; Hu, Wanpeng; Yan, Weina; Li, Yushan; He, Wei; Cao, Mengji; Zhang, Xiao; Luo, Bowen; Zhang, Suzhi

doi:10.1016/j.jplph.2021.153520

Cited by 12 publications

(8 citation statements)

References 44 publications

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“…Previous studies have shown that some XTH genes are involved in various abiotic stress responses in plants ( Cho et al., 2006a ; Du et al., 2021 ). The expression patterns of DlXTHs were further studied under different temperature conditions by analyzing the RNA-seq data for the longan EC ( Figure 3B ).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of the XTH gene family and functional analysis of DlXTH23.5/25 during early longan somatic embryogenesis

Chen²,

Liu³

et al. 2022

Front. Plant Sci.

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IntroductionXyloglucan endotransglucosylase (XET)/hydrolase (XTH) is a cell wall-modifying protein that affects cell expansion and loosening of the cell wall.ResultsThis study focused on the regulatory mechanism of DlXTH genes during early somatic embryogenesis (SE) and the heat stress response in longan. Mining of the available D. longan genome sequence yielded 25 putative XTH genes. Transcript profiles based on RNA sequencing (RNA-seq) data showed that most of the 17 detected DlXTH genes were highly expressed in the embryogenic callus (EC) (8) and globular embryo (GE) (8), and 13 of them responded significantly to heat stress. The assay for transposase-accessible chromatin sequencing (ATAC-seq) data analysis showed that in terms of chromatin accessibility, 22 of the 25 DlXTH genes were open during early SE, and most of the peak DlXTH genes with transcription differences during early SE were associated with high levels of H3K4me1. The most differentially expressed genes, DlXTH23.5 and DlXTH25, were selected for analysis. According to subcellular localization and quantitative real-time PCR (qRT-PCR) analysis, DlXTH23.5/25, which encode cell membrane-localized proteins, were expressed at the highest level in the GE and significantly responded to heat stress. Dual-luciferase assays and transient transformation showed that the transcription factors (TFs) DlWRKY31, DlERF1, and DlERF5 might bind to the DlXTH23.5/25 promoters to activate gene transcription. Transient overexpression of TFs and DlXTH23.5/25 induced XET activity in Nicotiana benthamiana leaves. Under heat stress in the longan EC, the XET activities and expression levels of TFs and DlXTH23.5/25 were significantly increased, and a high concentration of XET might inhibit longan SE.DiscussionsThus, the regulatory network composed of DlXTH23.5/25 and its related TFs may regulate early longan SE and participate in the regulatory pathway of longan under heat stress via cell wall repair through the action of XET.

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Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of the XTH gene family and functional analysis of DlXTH23.5/25 during early longan somatic embryogenesis

Chen²,

Liu³

et al. 2022

Front. Plant Sci.

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“…Additionally, ZmMATE1 and ZmMATE6 were previously reported to confer maize Al tolerance through citrate release (Maron et al, 2010; Du et al, 2021). Here, the transient assay in maize protoplasts and yeast one-hybrid analysis verified that ZmSTOP1-A directly binds to the Al-responsive GGNVS cis -element to activate ZmMATE1 and detoxify Al ( Figure 8B ).…”

Section: Discussionmentioning

confidence: 99%

“…After Al stress treatment, root exudate organic acids concentrations in Arabidopsis were measured and hematoxylin staining performed as previously described (Du et al, 2021). Root and shoot samples were collected separately and Al content qualified by inductively coupled plasma mass spectrometry (ICP-MS, PerkinElmer, NexlON 2000).…”

Section: Methodsmentioning

confidence: 99%

“…The genetic mechanisms underlying Al toxicity response of maize remain poorly understood. To date, only six genes have been reported contributed to Al tolerance in maize, by facilitating citric acid efflux ( ZmMATE1 and ZmMATE6 ) (Maron et al, 2010; Du et al, 2021), ROS elimination ( ZmALDH1 and ZmAT6 ) (Du et al, 2020; Du et al, 2022), cell wall fixation of Al ( ZmXTH1 ) (Du et al, 2021), and auxin transport ( ZmPGP1 ) (Zhang et al, 2018). In this study, ZmSTOP1-A was first identified in maize and its Al-responsive expression pattern was explored.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression ofZmSTOP1-AEnhances Aluminum Tolerance in Arabidopsis by Stimulating Organic Acid Secretion and Reactive Oxygen Species Scavenging

Liu,

Hu,

Zang

et al. 2023

Preprint

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Aluminum (Al) toxicity and low pH are major factors limiting plant growth in acidic soils. Sensitive to Proton Rhizotoxicity 1 (STOP1) transcription factor respond to these stresses by regulating the expression of multiple Al- or low pH- responsive genes. ZmSTOP1-A, a STOP1-like protein from maize (Zea mays), was localized to the nucleus and had transactivation activity. ZmSTOP1-A was expressed moderately in both roots and shoots of maize seedlings, but was not induced by Al stress or low pH. Overexpression of ZmSTOP1-A in Arabidopsis Atstop1 mutant partially restored Al tolerance and completely low pH tolerance with respect to root growth. Regarding Al tolerance, ZmSTOP1-A/Atstop1 plants showed clear upregulation of organic acid transporter genes, and leading to increased organic acid secretion and reduced Al accumulation in roots. Besides, the antioxidant enzyme activity in roots and shoots of ZmSTOP1-A/Atstop1 plants was significantly enhanced, ultimately alleviating Al toxicity via scavenging reactive oxygen species. Similarly, ZmSTOP1-A could directly activate ZmMATE1 expression in maize, positively correlated with the number of Al-responsive GGNVS cis-element in the ZmMATE1 promoter. Our results revealed that ZmSTOP1-A antagonizes Al toxicity by enhancing organic acid secretion and reactive oxygen species scavenging in Arabidopsis, demonstrating that it is an important transcription factor conferring Al tolerance. Our findings help to comprehensively elucidate the role of STOP1-like transcript factor in enabling plants to detoxifying Al.

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“…XTHs, encoding the essential catalytic enzyme XET and xyloglucan endohydrolase (XEH), are important for cell wall extensibility and remodeling. , By far, six genes including CsXTH14 , AtXTH14 , AtXTH15 , AtXTH17, AtXTH31 , and ZmXTH have been reported to be transcriptionally responsive to Al exposure, but only the latter two have been confirmed to confer Al tolerance. , AtXTH18 is the only gene having been confirmed to involve in Pb detoxification in plants . It appears that different XTH family members may have distinct roles in response to HMs.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Dynamics of Common Bean Roots Exposed to Various Heavy Metals Reveal Valuable Target Genes and Promoters for Genetic Engineering

Fang

Xia

et al. 2022

J. Agric. Food Chem.

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Understanding the gene regulatory basis of plant response to heavy metals (HMs) is fundamental for the management of food safety and security. However, a comprehensive and comparative view of the plant responses to different HMs is still lacking. Here, we compared root transcriptomes in common bean under 9 HM treatments at 50 μM for three time points each. Cd, Cr, Co, Ni, and Pb caused most severe morphological and/or biochemical retardations. A total of 448 genes were found to be responsive to all nine HMs, which were mostly involved in photosynthesis, oxidization–reduction, and ion binding. Cd and Cu triggered the greatest number of unique differentially expressed genes (DEG)s, which were predominantly related to cellular transport/localization in the former and RNA binding in the latter. Short-term and prolonged HM treatments shaped very different DEG patterns. Weighted gene co-expression network analysis identified six co-expression modules showing exceptionally high transcripts abundance in specific HM × time scenarios. We experimentally verified the promoter activity of the gene GIP1 and the novel function of XTH23 under Cu/Cd stress. Collectively, the transcriptomic atlas provides valuable resources for better understanding the common and unique mechanisms of plant response to different HMs and offers a mass of candidate target genes/promoters for genetic engineering.

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ZmXTH, a xyloglucan endotransglucosylase/hydrolase gene of maize, conferred aluminum tolerance in Arabidopsis

Cited by 12 publications

References 44 publications

Genome-wide analysis of the XTH gene family and functional analysis of DlXTH23.5/25 during early longan somatic embryogenesis

Genome-wide analysis of the XTH gene family and functional analysis of DlXTH23.5/25 during early longan somatic embryogenesis

Overexpression ofZmSTOP1-AEnhances Aluminum Tolerance in Arabidopsis by Stimulating Organic Acid Secretion and Reactive Oxygen Species Scavenging

Transcriptome Dynamics of Common Bean Roots Exposed to Various Heavy Metals Reveal Valuable Target Genes and Promoters for Genetic Engineering

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